Detailed information NTG0607169

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LA331_RS03215
• Genome accession: NZ_CP083658
• Coordinates: 653731..654909 (-)
• Length: 392 a.a.
• NCBI ID: WP_005235844.1
• Uniprot ID: N9NSE3
• Organism: Acinetobacter variabilis strain FDAARGOS_1487
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 648731..659909

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LA331_RS03195 (LA331_03195)	-	649159..650187 (+)	1029	WP_005235838.1	AraC family transcriptional regulator	-
LA331_RS03200 (LA331_03200)	gcvH	650270..650644 (+)	375	WP_004280423.1	glycine cleavage system protein GcvH	-
LA331_RS03205 (LA331_03205)	-	650884..652059 (+)	1176	WP_005235840.1	MFS transporter	-
LA331_RS03210 (LA331_03210)	pilU	652134..653330 (-)	1197	WP_005235842.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LA331_RS03215 (LA331_03215)	pilU	653731..654909 (-)	1179	WP_005235844.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LA331_RS03220 (LA331_03220)	-	655170..656219 (-)	1050	WP_005235846.1	3-deoxy-7-phosphoheptulonate synthase	-
LA331_RS03225 (LA331_03225)	-	656771..657301 (-)	531	WP_005235848.1	hypothetical protein	-
LA331_RS03230 (LA331_03230)	-	657510..658623 (-)	1114	Protein_626	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-

Sequence

Protein

• Length: 392 a.a.
• Molecular weight: 44500.07 Da
• Isoelectric Point: 6.7255

>NTDB_id=607169 LA331_RS03215 WP_005235844.1 653731..654909(-) (pilU) [Acinetobacter variabilis strain FDAARGOS_1487]
MYTTELLDEARKFMHHMLTKVVEYGGSDLFITADFPPSIKQQGLMKPFGQQKLTAEKTRLFAYALMNERQIQEFESVMEC
NFSISIPNVSRFRVNVFQQQHNVGMVIRIITTDIPSFEQLGLPASLQQVIMQKRGLVLVVGATGVGKSTTLAAMIDYRNT
HTEGHIITIEDPVEYVYQHKKSMITQREIGVDCVSWHNALINTMRQAPDVIVIGEIRDTDSMEHAIAFAESGHLCLTTLH
SNNADQALDRIINFFPEERRNQLLLDLSANIKAVISQRLIRTEDGKGRRAAVEILQNTPLVSDLIRKGEFHELKTIMARS
RELGMQTFDQALFDLYNQGIISYEEALRNADSSNELRLQIKLKSSRINPVLRAENAQISLLEQSRLRALSPD

Nucleotide

• Length: 1179 bp

>NTDB_id=607169 LA331_RS03215 WP_005235844.1 653731..654909(-) (pilU) [Acinetobacter variabilis strain FDAARGOS_1487]
ATGTATACAACTGAATTGCTCGATGAAGCACGTAAATTTATGCACCATATGCTGACCAAAGTCGTTGAATATGGTGGTTC
CGATCTTTTTATCACAGCTGATTTCCCCCCTAGTATCAAACAGCAGGGGTTAATGAAACCGTTTGGCCAGCAGAAACTCA
CAGCCGAAAAGACCCGTTTGTTTGCTTATGCCTTGATGAACGAGCGACAGATTCAGGAGTTTGAATCGGTGATGGAATGC
AATTTTAGTATTAGCATCCCGAATGTGTCTCGTTTCCGGGTTAATGTCTTCCAGCAACAGCACAATGTAGGCATGGTGAT
TAGGATCATTACGACAGATATTCCAAGCTTTGAGCAACTAGGCTTACCGGCTTCATTGCAACAGGTCATCATGCAGAAAC
GTGGTCTGGTGCTGGTAGTCGGCGCAACGGGTGTCGGAAAATCGACTACATTAGCAGCTATGATTGATTACCGTAATACG
CATACTGAAGGGCATATTATTACAATCGAAGATCCGGTGGAATATGTCTATCAGCATAAAAAATCGATGATTACCCAGCG
GGAAATTGGTGTGGATTGTGTTTCCTGGCACAATGCACTGATTAATACCATGCGGCAGGCACCTGACGTCATCGTGATTG
GTGAAATCCGCGATACAGACAGTATGGAACATGCAATTGCCTTTGCTGAATCCGGCCATTTATGTCTTACCACACTTCAT
TCCAATAATGCCGATCAGGCACTGGACCGGATTATTAACTTCTTCCCGGAAGAACGCCGTAATCAGCTATTGCTAGATTT
ATCAGCCAATATTAAAGCCGTGATTTCTCAGCGCCTAATTCGTACTGAAGATGGTAAAGGTCGTCGGGCAGCGGTAGAAA
TTCTGCAAAATACACCTTTAGTTTCTGACCTGATTCGTAAAGGTGAATTCCATGAACTTAAAACGATTATGGCTCGTTCG
CGGGAACTGGGCATGCAAACCTTTGATCAAGCGTTGTTTGATTTATATAACCAAGGCATTATTTCTTATGAAGAAGCCTT
GCGTAATGCAGACTCCAGCAATGAATTACGTCTACAAATCAAGCTAAAGAGCAGTCGTATTAATCCAGTCTTACGGGCAG
AAAATGCACAAATATCACTTCTGGAACAGTCCAGATTACGTGCACTTTCTCCTGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (64-282)

3D structure

• AlphaFold DB: N9NSE3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	59.483	88.776	0.528
pilU	Acinetobacter baylyi ADP1	54.085	90.561	0.49
pilU	Vibrio cholerae strain A1552	51.268	90.561	0.464
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.353	86.735	0.367