Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   K9N56_RS20540 Genome accession   NZ_CP083580
Coordinates   4005022..4006368 (-) Length   448 a.a.
NCBI ID   WP_006639265.1    Uniprot ID   M5NZS0
Organism   Bacillus sp. PM8313     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4000022..4011368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9N56_RS20505 (K9N56_20505) flgL 4000210..4001121 (-) 912 WP_006639258.1 flagellar hook-associated protein FlgL -
  K9N56_RS20510 (K9N56_20510) flgK 4001132..4002655 (-) 1524 WP_006639259.1 flagellar hook-associated protein FlgK -
  K9N56_RS20515 (K9N56_20515) - 4002673..4003155 (-) 483 WP_029419581.1 flagellar protein FlgN -
  K9N56_RS20520 (K9N56_20520) flgM 4003170..4003433 (-) 264 WP_006639261.1 flagellar biosynthesis anti-sigma factor FlgM -
  K9N56_RS20525 (K9N56_20525) - 4003513..4003932 (-) 420 WP_006639262.1 TIGR03826 family flagellar region protein -
  K9N56_RS20530 (K9N56_20530) comFC 4003990..4004724 (-) 735 WP_006639263.1 ComF family protein Machinery gene
  K9N56_RS20535 (K9N56_20535) - 4004681..4004965 (-) 285 WP_006639264.1 late competence development ComFB family protein -
  K9N56_RS20540 (K9N56_20540) comFA 4005022..4006368 (-) 1347 WP_006639265.1 DEAD/DEAH box helicase Machinery gene
  K9N56_RS20545 (K9N56_20545) - 4006561..4007403 (-) 843 WP_006639266.1 DegV family protein -
  K9N56_RS20550 (K9N56_20550) degU 4007523..4008212 (-) 690 WP_003185730.1 two-component system response regulator DegU Regulator
  K9N56_RS20555 (K9N56_20555) degS 4008294..4009451 (-) 1158 WP_006639267.1 sensor histidine kinase Regulator
  K9N56_RS20560 (K9N56_20560) - 4009673..4010311 (+) 639 WP_006639268.1 YigZ family protein -

Sequence

Protein


Download         Length: 448 a.a.        Molecular weight: 50591.30 Da        Isoelectric Point: 10.0116

>NTDB_id=606324 K9N56_RS20540 WP_006639265.1 4005022..4006368(-) (comFA) [Bacillus sp. PM8313]
MLESRHLLKSELPFPDHVIEWHIQKGLIKTEKPIAKTAKGFICKRCGQDQQTFFAKYPCFICDKTCVYCRSCVMMGRVSE
CTPLLTWKDADLPKWPAVRMEWRGVLSEGQEKAASSIVEAIRKKEELLIWAVCGAGKTEILFQGIEFALTKGLRVCIATP
RTDVVLELAPRLKNAIKGVEIAALYGGSPDRGMLSPLMISTTHQLLRYKEAFDVIIVDEVDAFPYCLDKKLQYAVKKAGK
QQCTRIYLTATPSREMKRHVGSGRLKAVQIPARYHRSPLPEPEFAWCGNWKKRLERKNIPSAVKNWLFKHKELDQPVFLF
VPSIQTLQSAVRLLKKEHFNTAGVHADDPDRNEKVKQFRSGAFDILVTTTILERGVTVKKAQVGVLGAESAVFTESALVQ
ISGRAGRHPQFPTGAVCFFHFGKTVNMIAARRHIQQMNKMAKLENLID

Nucleotide


Download         Length: 1347 bp        

>NTDB_id=606324 K9N56_RS20540 WP_006639265.1 4005022..4006368(-) (comFA) [Bacillus sp. PM8313]
ATGCTTGAGTCCCGCCACCTTCTCAAAAGCGAGTTGCCTTTTCCCGATCATGTGATTGAATGGCATATCCAAAAAGGTTT
GATAAAAACTGAAAAACCGATTGCAAAAACGGCAAAAGGCTTTATTTGCAAACGATGCGGACAGGATCAGCAGACGTTTT
TTGCTAAATACCCTTGTTTTATTTGTGATAAAACCTGCGTTTACTGCCGTTCATGCGTGATGATGGGGAGAGTAAGCGAA
TGTACGCCGCTCTTAACTTGGAAAGACGCTGACCTGCCCAAATGGCCGGCTGTCCGGATGGAGTGGAGAGGCGTTCTTTC
CGAGGGGCAGGAAAAAGCGGCAAGCTCGATTGTTGAAGCCATCCGCAAAAAAGAAGAGCTGTTAATTTGGGCGGTTTGCG
GTGCCGGTAAAACAGAGATTCTTTTTCAGGGAATAGAATTTGCTTTGACTAAAGGCTTGAGAGTATGTATAGCCACTCCG
AGAACAGATGTTGTTCTTGAGCTTGCCCCGAGATTAAAGAACGCTATTAAAGGAGTAGAAATTGCCGCTTTATACGGAGG
GAGTCCAGACAGGGGGATGCTCTCGCCTCTCATGATTTCTACCACCCACCAGCTTCTGCGCTACAAAGAAGCGTTCGATG
TGATCATTGTTGATGAGGTTGATGCATTTCCATATTGTTTGGATAAAAAGCTGCAATACGCCGTGAAAAAAGCGGGAAAG
CAGCAATGCACCCGAATATATTTAACGGCTACACCTTCACGGGAAATGAAACGGCATGTTGGATCCGGGAGGCTTAAGGC
CGTTCAGATTCCGGCAAGGTATCACAGAAGTCCATTGCCAGAACCGGAATTTGCATGGTGCGGCAACTGGAAAAAGAGAC
TTGAACGAAAAAACATTCCCTCCGCCGTGAAAAATTGGCTTTTCAAGCATAAAGAACTGGATCAGCCTGTCTTTTTATTT
GTGCCCTCGATTCAGACGCTTCAATCTGCGGTCAGGCTGTTGAAAAAGGAACATTTCAATACAGCGGGCGTACATGCGGA
TGACCCGGATAGAAATGAAAAGGTAAAGCAGTTTAGAAGCGGAGCGTTTGATATTCTCGTCACAACAACCATATTGGAAC
GGGGGGTAACGGTCAAAAAAGCCCAGGTCGGAGTGCTCGGTGCGGAGTCAGCCGTTTTTACAGAAAGCGCTCTTGTTCAA
ATATCGGGAAGAGCGGGGAGGCATCCGCAGTTTCCGACCGGAGCCGTTTGTTTTTTTCATTTCGGCAAGACGGTCAATAT
GATAGCCGCCCGCCGTCATATTCAACAAATGAATAAAATGGCTAAACTGGAAAATTTGATTGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(106-254)

Helicase conserved C-terminal domain (Helicase_C)

(303-408)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M5NZS0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

61.136

98.214

0.6
  comFA/cflA Streptococcus mitis SK321

37.9

97.768

0.371