Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMD59_RS19210 Genome accession   NZ_CP100883
Coordinates   3919237..3919677 (+) Length   146 a.a.
NCBI ID   WP_000360896.1    Uniprot ID   -
Organism   Escherichia coli strain ET896     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3914237..3924677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD59_RS19190 (NMD59_19060) aroP 3915235..3916608 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  NMD59_RS19195 (NMD59_19065) ampE 3916651..3917505 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NMD59_RS19200 (NMD59_19070) ampD 3917502..3918053 (-) 552 WP_000923727.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NMD59_RS19205 (NMD59_19075) nadC 3918141..3919034 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NMD59_RS19210 (NMD59_19080) pilA 3919237..3919677 (+) 441 WP_000360896.1 prepilin peptidase-dependent pilin Machinery gene
  NMD59_RS19215 (NMD59_19085) pilB 3919687..3921072 (+) 1386 WP_072777768.1 type II secretion system protein GspE Machinery gene
  NMD59_RS19220 (NMD59_19090) hofC 3921062..3922264 (+) 1203 WP_050010183.1 protein transport protein HofC -
  NMD59_RS19225 (NMD59_19095) guaC 3922299..3923342 (-) 1044 WP_284990060.1 GMP reductase -
  NMD59_RS19230 (NMD59_19100) - 3923498..3923542 (-) 45 WP_120795372.1 protein YacM -
  NMD59_RS19235 (NMD59_19105) coaE 3923567..3924187 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15647.84 Da        Isoelectric Point: 4.3938

>NTDB_id=606326 NMD59_RS19210 WP_000360896.1 3919237..3919677(+) (pilA) [Escherichia coli strain ET896]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQEILNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=606326 NMD59_RS19210 WP_000360896.1 3919237..3919677(+) (pilA) [Escherichia coli strain ET896]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCTGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAATTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363