Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   K8U54_RS12110 Genome accession   NZ_CP082928
Coordinates   2685882..2688050 (+) Length   722 a.a.
NCBI ID   WP_249910332.1    Uniprot ID   -
Organism   Pseudomonas fulva strain ZJU1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2680882..2693050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8U54_RS12090 (K8U54_12070) pilM 2683056..2684120 (+) 1065 WP_249910328.1 pilus assembly protein PilM Machinery gene
  K8U54_RS12095 (K8U54_12075) - 2684120..2684692 (+) 573 WP_249910329.1 PilN domain-containing protein -
  K8U54_RS12100 (K8U54_12080) pilO 2684689..2685312 (+) 624 WP_249910330.1 type 4a pilus biogenesis protein PilO -
  K8U54_RS12105 (K8U54_12085) pilP 2685309..2685827 (+) 519 WP_249910331.1 type 4a pilus biogenesis lipoprotein PilP -
  K8U54_RS12110 (K8U54_12090) pilQ 2685882..2688050 (+) 2169 WP_249910332.1 type IV pilus secretin PilQ Machinery gene
  K8U54_RS12115 (K8U54_12095) aroK 2688054..2688572 (+) 519 WP_075928589.1 shikimate kinase AroK -
  K8U54_RS12120 (K8U54_12100) aroB 2688624..2689730 (+) 1107 WP_249910333.1 3-dehydroquinate synthase -
  K8U54_RS12125 (K8U54_12105) - 2689738..2691360 (+) 1623 WP_249910334.1 AAA family ATPase -

Sequence

Protein


Download         Length: 722 a.a.        Molecular weight: 77327.55 Da        Isoelectric Point: 4.7648

>NTDB_id=603664 K8U54_RS12110 WP_249910332.1 2685882..2688050(+) (pilQ) [Pseudomonas fulva strain ZJU1]
MNSSLSRLSISLLVALLSPAALAANLQALDVAALPGDRVELKLSFDEPVAAPRGYTIEQPARIALDLPGVSNKLGVKNRE
LGMGNARSVTVVEAKDRTRLIINLTNLSPYNTRTEGNNLFVQVGTGVATSATTTAAAPVSAPAPATARPAQAATPVAAPA
APKVFGAVNKSISNIDFQRGEQGEGNVVINLSDASVSPDIQERGGKIILNFNRTQLPEALRVRLDVKDFATPVQFVSASG
TGDDASIVIEPVGAFDYLAYQTENKLTLSVKPLTQEDADKRQAERFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVAS
DTVAGNITLRLQNVPWDQALDLVLKTKGLDKRQVGNVLLVAPAEEIAARERQELESQKQIAELAPLRRELIQVNYAKAAD
MAKLFQSVTSANGGVADDRGSITVDERTNSIIAYQTQERLDELRRIVAQLDVAVRQVMIEARIVEANVNYDKALGVRWGG
SARRGGWDVSGKDGVQAFDEDTGARAPFVGTDTIGSTTLDDGLARLPFVDMGVANSTSGIGIGFLSNNVVLDLQLSAMES
SGNGEIVSQPKVVTSDKETAKILKGQEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFQRAVN
GVPPINKNEVNAKVLVSDGETIVIGGVFENTQSKSVEKVPFLGDVPYLGRLFRRDIVQDNKTELLVFITPRIMNTSAVAV
SR

Nucleotide


Download         Length: 2169 bp        

>NTDB_id=603664 K8U54_RS12110 WP_249910332.1 2685882..2688050(+) (pilQ) [Pseudomonas fulva strain ZJU1]
ATGAACAGCTCTCTCTCTCGCCTAAGCATCTCGCTGCTGGTGGCACTGCTCTCTCCTGCCGCTCTGGCCGCCAACCTGCA
GGCGCTGGACGTGGCGGCTCTGCCGGGCGACCGTGTCGAGCTGAAACTGTCCTTCGACGAGCCGGTAGCGGCGCCGCGCG
GCTACACCATCGAGCAGCCGGCGCGCATCGCCCTGGATCTGCCCGGCGTTTCCAACAAGCTGGGCGTGAAGAACCGGGAG
CTGGGCATGGGCAATGCGCGCAGCGTCACCGTGGTCGAGGCCAAGGATCGTACGCGGTTGATCATCAACCTGACCAACCT
GTCGCCCTACAATACCCGCACCGAAGGCAACAACCTGTTCGTGCAGGTCGGTACCGGCGTGGCCACGTCGGCAACCACCA
CGGCGGCAGCGCCAGTGAGCGCGCCTGCGCCAGCAACGGCCCGTCCTGCCCAGGCGGCGACGCCGGTTGCGGCACCGGCA
GCGCCGAAGGTGTTCGGCGCCGTCAACAAGTCGATCAGCAATATCGACTTTCAACGTGGCGAGCAGGGTGAGGGCAACGT
CGTCATCAACCTGTCCGATGCGTCCGTCAGCCCTGATATCCAGGAGCGTGGCGGCAAGATCATTCTCAATTTCAACCGCA
CCCAGCTGCCTGAAGCCCTGCGTGTGCGCCTGGACGTCAAGGACTTCGCCACCCCGGTGCAGTTCGTCAGTGCCAGCGGC
ACGGGTGATGATGCAAGCATCGTGATCGAGCCTGTTGGCGCTTTCGATTATCTGGCCTACCAGACCGAGAACAAGCTGAC
CCTCAGCGTCAAGCCGCTGACCCAGGAAGATGCCGACAAGCGTCAGGCCGAGCGCTTCGCCTATACCGGCGAGAAGCTGT
CGCTGAACTTCCAGGATATCGACGTGCGTTCGGTGCTGCAGCTGATCGCCGATTTCACCGATTTGAACCTGGTCGCCAGT
GATACGGTGGCGGGCAATATCACCCTGCGTCTGCAGAACGTTCCATGGGATCAGGCACTGGACCTGGTGCTCAAGACCAA
AGGCCTCGACAAGCGCCAGGTCGGCAACGTGCTGTTGGTAGCGCCGGCGGAAGAAATTGCCGCCCGTGAGCGTCAAGAGC
TGGAGTCGCAGAAGCAGATCGCCGAACTGGCCCCGCTGCGCCGTGAGCTGATTCAGGTCAACTACGCCAAGGCTGCCGAT
ATGGCCAAGCTGTTCCAGTCGGTGACCAGTGCCAATGGTGGCGTGGCGGATGATCGTGGCTCGATTACCGTCGATGAGCG
CACCAATAGCATCATCGCTTATCAGACTCAGGAGCGGCTGGATGAGCTGCGCCGTATCGTCGCTCAGCTGGATGTGGCGG
TGCGTCAGGTGATGATCGAGGCGCGTATCGTTGAGGCCAATGTCAATTATGATAAGGCGCTGGGCGTGCGTTGGGGAGGG
AGTGCAAGGAGAGGCGGCTGGGATGTAAGTGGTAAGGATGGTGTTCAGGCTTTTGATGAAGATACTGGTGCACGAGCTCC
ATTCGTAGGAACGGATACAATCGGTAGTACGACTTTGGATGATGGTCTTGCTCGCTTGCCTTTCGTTGATATGGGGGTAG
CTAATAGTACTTCTGGTATCGGTATAGGCTTCTTGAGTAACAATGTGGTACTTGATCTTCAGCTTTCTGCCATGGAAAGC
TCGGGTAACGGCGAAATTGTCTCTCAGCCCAAGGTGGTGACCTCAGATAAGGAAACCGCGAAGATTTTGAAGGGCCAAGA
GGTTCCCTATCAGGAAGCTAGCTCCAGTGGAGCAACGTCTACATCGTTTAAAGAAGCTGCCTTGTCTCTTGAAGTAACCC
CGCAAATCACGCCGGACAACCGCATTATCATGGAAGTAAAGGTGACCAAGGATGCACCGGACTTCCAGCGAGCAGTTAAC
GGAGTTCCGCCGATTAACAAAAATGAAGTCAATGCCAAGGTGCTCGTTTCTGATGGCGAGACGATCGTAATTGGTGGGGT
GTTCGAGAATACTCAGAGCAAATCTGTCGAAAAGGTTCCGTTTCTTGGGGATGTGCCGTATCTGGGCCGCCTGTTCCGCC
GCGATATCGTGCAGGACAACAAGACCGAGTTGCTGGTTTTCATCACTCCGCGCATCATGAACACCTCTGCGGTGGCCGTG
AGTCGGTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(316-362)

AMIN domain (AMIN)

(38-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(390-456)

Bacterial type II and III secretion system protein (Secretin)

(557-713)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

75.172

100

0.755
  comQ Acinetobacter baylyi ADP1

40.922

99.169

0.406
  pilQ Acinetobacter baumannii strain A118

38.22

100

0.404
  pilQ Acinetobacter baumannii D1279779

38.22

100

0.404