Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   K8U54_RS12090 Genome accession   NZ_CP082928
Coordinates   2683056..2684120 (+) Length   354 a.a.
NCBI ID   WP_249910328.1    Uniprot ID   -
Organism   Pseudomonas fulva strain ZJU1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2678056..2689120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8U54_RS12080 (K8U54_12060) - 2679055..2680323 (+) 1269 WP_249910326.1 malic enzyme-like NAD(P)-binding protein -
  K8U54_RS12085 (K8U54_12065) - 2680436..2682874 (-) 2439 WP_249910327.1 penicillin-binding protein 1A -
  K8U54_RS12090 (K8U54_12070) pilM 2683056..2684120 (+) 1065 WP_249910328.1 pilus assembly protein PilM Machinery gene
  K8U54_RS12095 (K8U54_12075) - 2684120..2684692 (+) 573 WP_249910329.1 PilN domain-containing protein -
  K8U54_RS12100 (K8U54_12080) pilO 2684689..2685312 (+) 624 WP_249910330.1 type 4a pilus biogenesis protein PilO -
  K8U54_RS12105 (K8U54_12085) pilP 2685309..2685827 (+) 519 WP_249910331.1 type 4a pilus biogenesis lipoprotein PilP -
  K8U54_RS12110 (K8U54_12090) pilQ 2685882..2688050 (+) 2169 WP_249910332.1 type IV pilus secretin PilQ Machinery gene
  K8U54_RS12115 (K8U54_12095) aroK 2688054..2688572 (+) 519 WP_075928589.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37979.29 Da        Isoelectric Point: 4.4260

>NTDB_id=603663 K8U54_RS12090 WP_249910328.1 2683056..2684120(+) (pilM) [Pseudomonas fulva strain ZJU1]
MLGLFSKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPANAVVEKNIAELEGVGQALSRVLAKAKTSVRSVSV
AVAGSAVITKTVEMEAGLSDDELENQLKLEADQYIPYPLEEVAIDFEVQGISPRNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYDLLAEQLGGAHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
DEAGLAKKQGGLPDDYESEVLQPFKDALVQQVSRSLQFFFAAGQYNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=603663 K8U54_RS12090 WP_249910328.1 2683056..2684120(+) (pilM) [Pseudomonas fulva strain ZJU1]
GTGCTAGGGCTCTTCAGTAAAAAAGCGAATACGCTTTTGGGGATCGATATAAGTTCGACCTCCGTCAAGCTCCTCGAATT
GAGTCGCTCAGGTAGCCGCTACAAGGTAGAGGCCTACGCTGTCGAGCCGCTCCCTGCCAACGCTGTGGTCGAAAAGAACA
TCGCCGAGCTCGAAGGGGTCGGCCAGGCCTTGTCCCGTGTCCTGGCCAAGGCCAAGACCAGCGTGCGCTCGGTCTCCGTG
GCCGTTGCCGGCTCCGCGGTGATCACCAAGACCGTCGAGATGGAAGCGGGCCTGTCGGACGACGAGCTGGAGAACCAGCT
CAAGCTCGAGGCCGACCAGTACATTCCGTACCCGCTTGAAGAGGTGGCGATCGACTTCGAAGTTCAGGGCATTTCGCCGC
GCAACCCCGAGCGGGTCGAAGTGCTGCTTGCCGCCTGCCGCAAGGAAAACGTCGAAGTCCGCGAGGCCGCCCTGGCCCTC
GCCGGGCTGACCGCCAAGGTCGTCGATGTCGAAGCCTATGCCCTCGAGCGTGCCTACGACCTGCTGGCCGAGCAGCTCGG
CGGCGCACACGACGAGTTGACCGTGGCCGTCGTCGACATCGGCGCGACCATGACCACGCTCAGCGTGCTGCACAATGGCC
GCACCATCTACACCCGCGAGCAGCTGTTCGGTGGCCGCCAGCTGACCGAGGAAATTCAGCGTCGCTATGGCCTGTCCGTC
GATGAGGCCGGGCTTGCCAAGAAGCAGGGCGGCCTGCCCGACGACTACGAAAGCGAAGTGCTGCAGCCGTTCAAGGATGC
ATTGGTACAGCAGGTGTCGCGCTCGCTGCAGTTCTTCTTCGCCGCCGGCCAGTACAACGATGTGGATTACATCCTGCTGG
CCGGTGGCACCGCTTCCATCCCCGATCTCGACCGCCTTATCCAGCAGAAGATCGGTACCCAGACGCTGGTCGCCAACCCC
TTCGCGGACATGGCCCTGAGCGGCAAGGTGAATGCGGGAGCGCTGGCCAGTGATGCACCGGCATTGATGATTGCCTGCGG
CCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.056

100

0.562
  comM Acinetobacter nosocomialis M2

56.056

100

0.562
  comM Acinetobacter baylyi ADP1

52.825

100

0.528
  pilM Legionella pneumophila strain ERS1305867

46.328

100

0.463