Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   K8O89_RS14885 Genome accession   NZ_CP082852
Coordinates   3329854..3330453 (+) Length   199 a.a.
NCBI ID   WP_019233702.1    Uniprot ID   A0AAX0WVD2
Organism   Legionella anisa strain FDAARGOS_1480     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3324854..3335453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8O89_RS14870 (K8O89_14840) - 3326848..3327966 (-) 1119 WP_019233705.1 hypothetical protein -
  K8O89_RS14875 (K8O89_14845) pilM 3328211..3329275 (+) 1065 WP_040522622.1 pilus assembly protein PilM Machinery gene
  K8O89_RS14880 (K8O89_14850) pilN 3329282..3329857 (+) 576 WP_019233703.1 PilN domain-containing protein Machinery gene
  K8O89_RS14885 (K8O89_14855) pilO 3329854..3330453 (+) 600 WP_019233702.1 type 4a pilus biogenesis protein PilO Machinery gene
  K8O89_RS14890 (K8O89_14860) pilP 3330450..3330965 (+) 516 WP_019233701.1 pilus assembly protein PilP Machinery gene
  K8O89_RS14895 (K8O89_14865) pilQ 3330974..3333079 (+) 2106 WP_058388524.1 type IV pilus secretin PilQ Machinery gene
  K8O89_RS14900 (K8O89_14870) aroK 3333347..3333874 (+) 528 WP_019233700.1 shikimate kinase AroK -
  K8O89_RS14905 (K8O89_14875) aroB 3333861..3334991 (+) 1131 WP_019233699.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22900.83 Da        Isoelectric Point: 7.9973

>NTDB_id=603058 K8O89_RS14885 WP_019233702.1 3329854..3330453(+) (pilO) [Legionella anisa strain FDAARGOS_1480]
MNNINLNELTLENVGQWPLVVKICVLIGVSILIIALGYWLIIQDNFTQFNSLKGQEATLKTDFENKQRQLFNLPAYRLQL
QVMMERFALMLKQLPEKNQMPGLLEDISKTGVASGLRFELFAPQPEVVHDFYVERPIKISVVGTYFQLAMFISRVAEMNR
IVTLHDFTIKGVSSKDNKVVSEDELVMNITAKIYRYRAQ

Nucleotide


Download         Length: 600 bp        

>NTDB_id=603058 K8O89_RS14885 WP_019233702.1 3329854..3330453(+) (pilO) [Legionella anisa strain FDAARGOS_1480]
ATGAATAACATTAATCTGAATGAATTAACTCTGGAAAATGTTGGGCAATGGCCATTAGTGGTTAAAATTTGCGTTCTTAT
AGGAGTGAGTATTTTAATTATTGCTTTGGGTTACTGGCTAATCATACAAGACAATTTCACTCAGTTTAATAGTCTAAAAG
GGCAGGAGGCTACTTTAAAAACAGATTTTGAAAACAAGCAACGTCAATTATTTAATTTGCCGGCCTATCGCCTGCAGTTG
CAAGTTATGATGGAGCGTTTTGCATTAATGCTCAAACAACTTCCAGAAAAAAATCAAATGCCAGGTTTATTAGAAGATAT
TTCCAAAACAGGAGTAGCATCTGGCTTGAGGTTTGAATTATTTGCGCCACAACCTGAGGTAGTACATGATTTTTATGTTG
AGCGACCAATAAAAATTTCAGTAGTCGGAACTTATTTTCAATTGGCAATGTTTATTAGTCGGGTCGCTGAGATGAATCGT
ATTGTAACCTTGCATGACTTCACTATAAAAGGGGTGTCCTCCAAAGATAATAAAGTGGTTTCGGAGGATGAATTGGTGAT
GAACATCACCGCTAAAATATATCGATATCGCGCACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(51-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

72.864

100

0.729
  pilO Acinetobacter baumannii D1279779

40.415

96.985

0.392
  comO Acinetobacter baylyi ADP1

40.314

95.98

0.387