Detailed information NTG0602954

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: K8P08_RS13835
• Genome accession: NZ_CP082847
• Coordinates: 3089348..3090412 (-)
• Length: 354 a.a.
• NCBI ID: WP_010946662.1
• Uniprot ID: Q5ZX06
• Organism: Legionella pneumophila strain FDAARGOS_1483
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3084348..3095412

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K8P08_RS13810 (K8P08_13850)	aroK	3084526..3085053 (-)	528	WP_010946667.1	shikimate kinase AroK	-
K8P08_RS13815 (K8P08_13855)	pilQ	3085499..3087595 (-)	2097	WP_010946666.1	type IV pilus secretin PilQ	Machinery gene
K8P08_RS13820 (K8P08_13860)	pilP	3087599..3088186 (-)	588	WP_010946665.1	pilus assembly protein PilP	Machinery gene
K8P08_RS13825 (K8P08_13865)	pilO	3088183..3088782 (-)	600	WP_014841277.1	type 4a pilus biogenesis protein PilO	Machinery gene
K8P08_RS13830 (K8P08_13870)	pilN	3088796..3089344 (-)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
K8P08_RS13835 (K8P08_13875)	pilM	3089348..3090412 (-)	1065	WP_010946662.1	type IV pilus assembly protein PilM	Machinery gene
K8P08_RS13840 (K8P08_13880)	ravI	3090829..3091839 (+)	1011	WP_010946661.1	Dot/Icm T4SS effector RavI	-
K8P08_RS13845 (K8P08_13885)	-	3091991..3094375 (+)	2385	WP_010946660.1	penicillin-binding protein 1A	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38732.71 Da
• Isoelectric Point: 5.0367

>NTDB_id=602954 K8P08_RS13835 WP_010946662.1 3089348..3090412(-) (pilM) [Legionella pneumophila strain FDAARGOS_1483]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNIIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQSGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=602954 K8P08_RS13835 WP_010946662.1 3089348..3090412(-) (pilM) [Legionella pneumophila strain FDAARGOS_1483]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATCCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGCGAAGAAATACCTGCCTCTGCCTTAGAAGGAAATATAA
TTAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATCGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAATCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5ZX06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.435	100	0.994
comM	Acinetobacter nosocomialis M2	42.817	100	0.429
pilM	Acinetobacter baumannii D1279779	42.535	100	0.427
comM	Acinetobacter baylyi ADP1	41.457	100	0.418