Detailed information NTG0601447

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: NC516_RS01900
• Genome accession: NZ_CP099485
• Coordinates: 400356..401363 (+)
• Length: 335 a.a.
• NCBI ID: WP_011374076.1
• Uniprot ID: Q38YQ9
• Organism: Latilactobacillus sakei strain WiKim0095
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 395356..406363

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NC516_RS01880 (NC516_01880)	hexB	396399..398360 (+)	1962	WP_146105228.1	DNA mismatch repair endonuclease MutL	Machinery gene
NC516_RS01885 (NC516_01885)	-	398360..398905 (+)	546	WP_099947252.1	Maf family protein	-
NC516_RS01890 (NC516_01890)	-	398918..399481 (+)	564	WP_197704868.1	TIGR00730 family Rossman fold protein	-
NC516_RS01895 (NC516_01895)	ruvA	399732..400343 (+)	612	WP_099947253.1	Holliday junction branch migration protein RuvA	Machinery gene
NC516_RS01900 (NC516_01900)	ruvB	400356..401363 (+)	1008	WP_011374076.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
NC516_RS01905 (NC516_01905)	queA	401378..402409 (+)	1032	WP_105300141.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
NC516_RS01910 (NC516_01910)	-	402436..402957 (+)	522	WP_252814952.1	GNAT family protein	-
NC516_RS01915 (NC516_01915)	arcA	403163..404398 (+)	1236	WP_016264645.1	arginine deiminase	-
NC516_RS01920 (NC516_01920)	argF	404424..405440 (+)	1017	WP_011374080.1	ornithine carbamoyltransferase	-

Sequence

Protein

• Length: 335 a.a.
• Molecular weight: 37265.71 Da
• Isoelectric Point: 4.8413

>NTDB_id=601447 NC516_RS01900 WP_011374076.1 400356..401363(+) (ruvB) [Latilactobacillus sakei strain WiKim0095]
MADERIVSAENDDFAEASIEKTLRPQVLAQYIGQDRVKNELAVYIEAAKKREESLDHVLLYGPPGLGKTTLAMVIANELQ
VQIRTTSGPAIERPGDLVALLNELQPGDVLFIDEIHRLPKMVEELLYSAMEDFYIDIVVGQGPTAHPVHFPLPPFTLIGA
TTRAGLLSAPLRDRFGIVEHMAYYTEADLMDIVQRSAGVFNMSIVPDGALEIARRSRGTPRIANRLLKRTRDYAQVADQN
TIDQAIADHALSQLQVDIRGLDGVDRKILQMMIDYYQGGPVGLKTIAANIGEENETIEEVYEPYLLQIGFLKRTQRGRMV
TPAGYAHLGMPYPEK

Nucleotide

• Length: 1008 bp

>NTDB_id=601447 NC516_RS01900 WP_011374076.1 400356..401363(+) (ruvB) [Latilactobacillus sakei strain WiKim0095]
ATGGCAGATGAGCGAATTGTTTCAGCCGAAAACGATGATTTTGCTGAAGCAAGTATTGAAAAAACATTGCGGCCACAAGT
CCTTGCACAATATATCGGTCAAGATCGGGTTAAAAATGAGTTAGCGGTCTATATTGAAGCGGCCAAAAAACGGGAAGAGT
CCCTCGATCACGTTTTACTCTATGGCCCACCAGGTTTAGGGAAAACGACGTTAGCGATGGTGATTGCTAACGAGTTGCAA
GTCCAGATTCGAACGACGAGTGGCCCTGCAATCGAGCGGCCTGGGGATTTAGTGGCGTTATTAAACGAGTTACAACCCGG
GGATGTTTTATTTATTGATGAAATTCATCGTTTACCTAAGATGGTTGAAGAATTACTTTATTCAGCGATGGAAGATTTCT
ACATCGATATTGTGGTTGGTCAAGGGCCTACCGCACATCCCGTCCATTTTCCATTACCACCGTTCACATTGATTGGCGCG
ACGACGCGGGCGGGCTTATTGTCTGCGCCATTGCGAGATCGTTTCGGAATTGTGGAACATATGGCTTACTACACGGAAGC
TGATTTGATGGACATTGTGCAACGCTCAGCCGGCGTCTTTAACATGTCGATTGTGCCAGATGGCGCGTTAGAAATCGCAC
GTCGCTCGCGAGGGACGCCGCGGATCGCCAATCGGTTGTTGAAACGAACTCGAGATTACGCACAAGTGGCTGACCAAAAT
ACGATTGATCAGGCGATTGCTGATCACGCATTAAGTCAGCTACAAGTTGATATTCGGGGCTTAGACGGTGTCGATCGCAA
AATCCTCCAGATGATGATTGATTATTATCAAGGCGGACCAGTCGGTTTAAAAACCATTGCCGCCAATATTGGTGAAGAAA
ACGAAACAATTGAAGAAGTTTACGAGCCTTATTTACTGCAAATTGGCTTTTTAAAACGAACGCAAAGAGGCCGGATGGTA
ACGCCGGCTGGTTATGCCCATTTAGGCATGCCTTATCCAGAAAAATAG

Domains

Predicted by InterProScan.

• RuvB AAA lid domain (AAA_lid_4) (184-257)

• RuvB C-terminal winged helix domain (WHD_RuvB) (260-328)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

3D structure

• AlphaFold DB: Q38YQ9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	64.653	98.806	0.639
ruvB	Streptococcus pneumoniae R6	64.688	95.522	0.618
ruvB	Streptococcus pneumoniae D39	64.688	95.522	0.618
ruvB	Streptococcus pneumoniae TIGR4	64.375	95.522	0.615
ruvB	Synechocystis sp. PCC 6803	58.28	93.731	0.546
ruvB	Helicobacter pylori 26695	51.829	97.91	0.507