Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   M5C73_RS10305 Genome accession   NZ_CP098790
Coordinates   2096166..2098049 (-) Length   627 a.a.
NCBI ID   WP_195229226.1    Uniprot ID   -
Organism   Lactococcus lactis strain WiKim0124     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Genomic Context

Location: 2091166..2103049
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5C73_RS10275 (M5C73_10275) - 2091537..2092217 (-) 681 WP_017864968.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  M5C73_RS10280 (M5C73_10280) macP 2092249..2092527 (-) 279 WP_003130796.1 cell wall synthase accessory phosphoprotein MacP -
  M5C73_RS10285 (M5C73_10285) - 2092520..2093104 (-) 585 WP_012898439.1 NUDIX hydrolase -
  M5C73_RS10290 (M5C73_10290) glmU 2093209..2094585 (-) 1377 WP_003130798.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -
  M5C73_RS10295 (M5C73_10295) proC 2094813..2095601 (+) 789 WP_250354258.1 pyrroline-5-carboxylate reductase -
  M5C73_RS10300 (M5C73_10300) rpsO 2095680..2095949 (-) 270 WP_015427039.1 30S ribosomal protein S15 -
  M5C73_RS10305 (M5C73_10305) pknB 2096166..2098049 (-) 1884 WP_195229226.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  M5C73_RS10310 (M5C73_10310) - 2098049..2098825 (-) 777 WP_003130802.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  M5C73_RS10315 (M5C73_10315) rsmB 2098906..2100180 (-) 1275 WP_014570715.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  M5C73_RS10320 (M5C73_10320) - 2100379..2100885 (-) 507 WP_012898444.1 Asp23/Gls24 family envelope stress response protein -
  M5C73_RS10325 (M5C73_10325) - 2100906..2101094 (-) 189 WP_012898445.1 DUF2273 domain-containing protein -
  M5C73_RS10330 (M5C73_10330) amaP 2101105..2101665 (-) 561 WP_010906186.1 alkaline shock response membrane anchor protein AmaP -
  M5C73_RS10335 (M5C73_10335) - 2101693..2101935 (-) 243 WP_003131476.1 GlsB/YeaQ/YmgE family stress response membrane protein -

Sequence

Protein


Download         Length: 627 a.a.        Molecular weight: 67722.00 Da        Isoelectric Point: 4.8276

>NTDB_id=601180 M5C73_RS10305 WP_195229226.1 2096166..2098049(-) (pknB) [Lactococcus lactis strain WiKim0124]
MIQIGKIFADRYRIIKEIGRGGMANVYQGEDTFLGDRLVAIKVLRSNFENDDIAIARFQREAFAMAELSHPNIVGISDVG
EFESQQYIVMEFVDGMTLKQYINQNAPLANDEAIEIITEILSAMDMAHSHGIIHRDLKPQNVLVSSSGTVKVTDFGIAKA
LSETSLTQTNTMFGSVHYLSPEQARGSNATVQSDIYAIGIILFELLTGQIPFDGDSAVAIALKHFQESIPSIINLNPEVP
QALENVVIKATAKDIKNRYNDVEEMMTDVATSTSLDRRGEEKLVFNKDHDETKIMPANLINPYDTKPLIDKKEDNDSQTD
EKAASSEVGNKNKKSKKGLIIGLIVLLLVVGGATLAWVVSTPTNVKIPNVTNSKLDQAKSKIKDAKLKVGTVHKQQSSTI
AEGNVIKTDPTSGTTVRSNSSVDIYVSTGNEDIIKMKDFVGEKIDEAMATLLKDYGIDESQVTQTSVPSDSYPAGTIIKQ
SPKKGSSFDTKGSEKITFEVSSGKQISIPDYTNQDAESYKAMLKQAGFTNITLNYIDTPGSPTGWVFGVTPSPDGGKVSP
DTPIVVKVTQAVAQSSSVPVSTSESSTSTFESSSQVSTTQSSESSSQVSTTQSSDSSGSQTSTTTTR

Nucleotide


Download         Length: 1884 bp        

>NTDB_id=601180 M5C73_RS10305 WP_195229226.1 2096166..2098049(-) (pknB) [Lactococcus lactis strain WiKim0124]
ATGATTCAAATCGGTAAAATCTTTGCCGATCGTTATCGGATTATCAAAGAAATCGGACGTGGCGGAATGGCGAACGTTTA
TCAAGGTGAAGATACTTTTCTTGGTGACCGCTTAGTTGCAATAAAAGTGCTACGTTCAAATTTTGAAAACGATGATATCG
CGATTGCTCGTTTTCAGCGAGAAGCATTCGCCATGGCAGAACTTTCACATCCAAATATCGTAGGTATTTCTGATGTTGGT
GAATTTGAAAGTCAACAATACATTGTCATGGAATTTGTTGACGGCATGACACTTAAACAATACATCAATCAAAATGCACC
TTTAGCCAATGATGAAGCTATTGAAATTATTACTGAAATTTTATCAGCAATGGACATGGCCCATTCACATGGTATTATTC
ACCGGGATTTAAAACCACAAAATGTTTTAGTTTCCAGTAGTGGAACTGTAAAAGTAACGGATTTTGGAATTGCGAAAGCA
CTCTCTGAAACTTCACTTACTCAAACAAATACAATGTTTGGTTCGGTTCATTATTTATCACCAGAGCAAGCGCGTGGTTC
AAATGCAACTGTTCAATCAGATATTTATGCGATTGGAATTATTTTATTTGAACTTTTAACAGGTCAAATTCCATTTGATG
GAGATTCTGCAGTTGCTATTGCTCTAAAACATTTCCAAGAGAGTATTCCAAGCATCATTAATCTCAATCCAGAAGTTCCA
CAAGCTTTGGAAAATGTTGTCATTAAAGCTACAGCAAAAGATATTAAAAATCGCTATAATGATGTAGAAGAAATGATGAC
TGATGTGGCAACTTCAACGAGTCTTGACCGTCGTGGGGAAGAAAAATTAGTTTTCAATAAGGATCATGATGAAACAAAAA
TTATGCCTGCCAATCTAATTAATCCTTATGACACTAAGCCTCTGATTGATAAAAAAGAAGATAATGATTCACAAACAGAT
GAAAAAGCTGCAAGCTCAGAAGTAGGAAATAAAAACAAAAAATCTAAAAAAGGCTTGATTATTGGTCTTATTGTCCTTCT
TTTAGTTGTTGGCGGTGCTACTTTAGCTTGGGTTGTCTCAACACCAACTAATGTTAAGATACCTAATGTGACAAACTCGA
AACTTGACCAAGCAAAAAGCAAAATTAAAGATGCAAAACTTAAAGTGGGAACAGTTCATAAACAACAAAGCTCAACGATT
GCTGAAGGAAATGTCATCAAGACTGATCCAACTAGTGGAACAACCGTGAGATCTAATAGCTCCGTTGATATTTATGTTTC
TACGGGAAATGAAGATATCATCAAAATGAAGGACTTTGTCGGTGAAAAGATTGATGAGGCGATGGCAACATTACTTAAGG
ATTATGGTATTGATGAAAGCCAAGTCACTCAAACTTCCGTTCCTAGTGATTCTTACCCAGCAGGTACAATTATTAAACAA
AGCCCTAAAAAAGGTTCTAGTTTTGATACCAAAGGCAGTGAAAAAATTACCTTTGAAGTTTCAAGTGGTAAGCAAATTTC
CATTCCTGATTATACAAATCAGGATGCTGAAAGTTATAAAGCAATGTTGAAACAAGCTGGCTTTACTAATATTACTTTGA
ATTATATTGATACTCCGGGGAGTCCAACAGGCTGGGTCTTTGGAGTAACACCTTCTCCTGATGGCGGAAAGGTTTCGCCG
GATACTCCGATTGTTGTAAAAGTTACTCAAGCAGTAGCGCAGTCTTCATCAGTACCTGTTTCTACATCGGAAAGTTCAAC
TTCAACTTTTGAATCATCTTCACAAGTTTCAACAACACAATCATCAGAATCATCTTCACAAGTTTCAACAACACAATCAT
CAGATTCTTCAGGTTCTCAAACTTCAACAACGACAACTAGATAA

Domains

Predicted by InterProScan.

PASTA domain (PASTA)

(508-569)

PASTA domain (PASTA)

(365-428)

PASTA domain (PASTA)

(434-501)

Protein kinase domain (Pkinase)

(12-259)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

45.057

98.405

0.443
  stkP/pknB Streptococcus salivarius strain HSISS4

49.267

87.081

0.429
  stkP Streptococcus pneumoniae TIGR4

46.328

84.689

0.392
  stkP Streptococcus pneumoniae D39

46.139

84.689

0.391
  stkP Streptococcus pneumoniae R6

46.139

84.689

0.391
  stkP Streptococcus pneumoniae Rx1

45.951

84.689

0.389