Detailed information NTG0600517

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: K6751_RS26425
• Genome accession: NZ_CP082244
• Coordinates: 5768901..5769965 (-)
• Length: 354 a.a.
• NCBI ID: WP_044406728.1
• Uniprot ID: A0A0D6SQX4
• Organism: Metapseudomonas otitidis strain CSMC7.1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5763901..5774965

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6751_RS26400 (K6751_26400)	aroK	5764516..5765034 (-)	519	WP_074973697.1	shikimate kinase AroK	-
K6751_RS26405 (K6751_26405)	pilQ	5765040..5767124 (-)	2085	WP_107331193.1	type IV pilus secretin PilQ	Machinery gene
K6751_RS26410 (K6751_26410)	pilP	5767179..5767709 (-)	531	WP_107331194.1	type 4a pilus biogenesis lipoprotein PilP	-
K6751_RS26415 (K6751_26415)	pilO	5767709..5768332 (-)	624	WP_222900450.1	type 4a pilus biogenesis protein PilO	-
K6751_RS26420 (K6751_26420)	pilN	5768329..5768901 (-)	573	WP_222900451.1	type 4a pilus biogenesis protein PilN	-
K6751_RS26425 (K6751_26425)	pilM	5768901..5769965 (-)	1065	WP_044406728.1	pilus assembly protein PilM	Machinery gene
K6751_RS26430 (K6751_26430)	-	5770339..5772570 (+)	2232	WP_411828671.1	penicillin-binding protein 1A	-
K6751_RS26435 (K6751_26435)	-	5772676..5773944 (-)	1269	WP_074973678.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37701.00 Da
• Isoelectric Point: 4.3980

>NTDB_id=600517 K6751_RS26425 WP_044406728.1 5768901..5769965(-) (pilM) [Metapseudomonas otitidis strain CSMC7.1]
MLGLFNKKANSLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVVAKAKAGSKLAAV
AVAGSAVITKTIEMDAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGVSARNPERMDVLLAACRRENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLASQLGNGSEDLTVAIVDIGATMTTLSVLNNGRTIYSREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQYNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMALSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=600517 K6751_RS26425 WP_044406728.1 5768901..5769965(-) (pilM) [Metapseudomonas otitidis strain CSMC7.1]
GTGCTAGGGCTCTTCAACAAGAAAGCGAATTCGCTGCTGGGGATCGACATAAGCTCGACCTCGGTCAAGCTCCTCGAATT
GAGTCGCTCGGGTGGCCGCTACAAGGTAGAGGCCTACGCCGTCGAGCCGCTCCCGCCAAATGCTGTCGTCGAAAAGAACA
TTGCTGAGCTGGAAGGCGTCGGCCAGGCACTGTCGCGTGTGGTCGCCAAGGCGAAGGCAGGCTCCAAGCTGGCAGCCGTT
GCTGTTGCGGGTTCGGCCGTCATCACCAAGACGATCGAGATGGATGCGGGGTTGTCCGATGACGAGCTGGAGAACCAGCT
CAAGATCGAAGCTGACCAGTACATTCCCTATCCCCTTGAAGAAGTAGCCATCGATTTCGAGGTTCAGGGCGTTTCTGCAC
GAAACCCCGAGCGAATGGATGTGCTGCTGGCAGCCTGCCGTCGCGAGAACGTCGAAGTGCGCGAGGCAGCGCTTGCTCTC
GCCGGGCTCACCGCGAAAGTCGTGGATGTCGAGGCCTATGCGCTTGAGCGCGCCTATGGGCTCCTTGCTTCCCAGTTGGG
GAATGGATCGGAGGATCTGACGGTTGCGATCGTGGACATCGGCGCGACGATGACCACCCTGAGCGTCTTGAACAATGGCC
GCACCATATACAGTCGCGAGCAGTTGTTCGGAGGTCGCCAGTTGACCGAAGAAATTCAGCGCCGCTATGGCCTTTCTGTC
GAGGAAGCGGGCTTGGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAGGTTTTGCAACCGTTCAAAGAGGC
AGTTGTCCAGCAAGTCTCACGGTCGCTGCAGTTCTTTTTTGCAGCGGGCCAGTACAACGATGTCGACTACATCCTCCTGG
CTGGCGGGACGGCCTCTATCCCTGATCTGGATCGCCTGATCCAGCAGAAGATCGGAACGCCGACGCTGGTCGCAAACCCC
TTCGCCGACATGGCACTGAGCGGCAAAGTCAACGCAGGTGCGCTGGCAAGTGATGCGCCGGCCTTGATGATCGCTTGTGG
CTTGGCAATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0D6SQX4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	57.062	100	0.571
comM	Acinetobacter nosocomialis M2	57.062	100	0.571
comM	Acinetobacter baylyi ADP1	54.237	100	0.542
pilM	Legionella pneumophila strain ERS1305867	48.023	100	0.48