Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   K5Y35_RS05280 Genome accession   NZ_CP082236
Coordinates   1202997..1204061 (-) Length   354 a.a.
NCBI ID   WP_123470753.1    Uniprot ID   -
Organism   Pseudomonas sp. SC3(2021)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1197997..1209061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5Y35_RS05255 aroK 1198669..1199187 (-) 519 WP_045122260.1 shikimate kinase AroK -
  K5Y35_RS05260 pilQ 1199192..1201273 (-) 2082 WP_222833824.1 type IV pilus secretin PilQ Machinery gene
  K5Y35_RS05265 - 1201287..1201814 (-) 528 WP_222833825.1 pilus assembly protein PilP -
  K5Y35_RS05270 pilO 1201811..1202434 (-) 624 WP_123470756.1 type 4a pilus biogenesis protein PilO -
  K5Y35_RS05275 - 1202431..1202997 (-) 567 WP_123470754.1 PilN domain-containing protein -
  K5Y35_RS05280 pilM 1202997..1204061 (-) 1065 WP_123470753.1 pilus assembly protein PilM Machinery gene
  K5Y35_RS05285 - 1204281..1206725 (+) 2445 WP_409202052.1 penicillin-binding protein 1A -
  K5Y35_RS05290 - 1207453..1208721 (-) 1269 WP_003220889.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38151.74 Da        Isoelectric Point: 4.8182

>NTDB_id=600341 K5Y35_RS05280 WP_123470753.1 1202997..1204061(-) (pilM) [Pseudomonas sp. SC3(2021)]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPANAVVEKNIAELEGVGHALSRVLVKARTGLKSVAV
AVAGSAVITKIIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVSVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAVSQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLNTPTQVANP
FFDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=600341 K5Y35_RS05280 WP_123470753.1 1202997..1204061(-) (pilM) [Pseudomonas sp. SC3(2021)]
GTGCTGGGACTCTTCAATAAAAAGGCCAATACGTTACTGGGGATCGACATCAGCTCCACTTCGGTCAAGTTGCTGGAACT
GAGCCGTCAGGGTGATCGCTACCGGGTCGAAGCGTACGCGGTGGAGCCGTTGCCCGCGAATGCCGTGGTCGAGAAAAACA
TCGCCGAACTCGAAGGCGTCGGGCACGCCCTGAGCCGGGTGCTGGTCAAGGCGCGCACCGGGCTCAAGAGCGTGGCCGTG
GCCGTGGCCGGTTCAGCGGTGATCACCAAGATCATCGAAATGGATGCCGGACTGTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATTCCCTACCCGCTGGACGAAGTGGCCATCGACTTCGAAGTCCAGGGCGTGTCCCCGC
GCAATCCCGAGCGCGTCAGCGTGCTGCTCGCCGCCTGTCGCAAGGAAAACGTCGAAGTTCGCGAGGCGGCGCTGGCCCTC
GCCGGGTTGACGGCGCGCGTGGTGGATGTCGAGGCCTACGCGCTGGAGCGCTCGTTCGGCCTGCTTGCCACGCAACTGGC
GGTGTCCCAGGAGCGTCTGACCGTGGCCGTGGTCGACATCGGCGCGACCATGACCACCCTCAGCGTCCTGCACAACGGCA
AGATCATCTACACCCGCGAGCAATTGTTCGGCGGCCGGCAACTGACCGAGGAAATCCAGCGGCGTTATGGCCTGACGGTC
GAACAGGCCGGGTTGGCGAAAAAGCAGGGCGGACTGCCCGACGATTACGTCAGCGAGGTCTTGCAGCCGTTTCGCGAGGC
ACTGGTACAGCAGGTGTCGCGCTCGTTGCAGTTCTTTTTCGCTTCTGGCCAGTACAACGCGGTCGATCACATTTTATTGG
CCGGCGGCACGGCCTCGGTGCCGGGTCTGGATCGTTTGATCGAGCAGCGCCTGAACACCCCGACCCAGGTCGCCAACCCG
TTTTTCGACATGGCCCTGAGCAGCAAGGTCAACGCCGGGGCCCTGGCCAGTGATGCGCCGGCCCTGATGATTGCCTGCGG
GCTCGCGCTGCGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

55.367

100

0.554
  pilM Legionella pneumophila strain ERS1305867

46.893

100

0.469