Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   K5Y35_RS05260 Genome accession   NZ_CP082236
Coordinates   1199192..1201273 (-) Length   693 a.a.
NCBI ID   WP_222833824.1    Uniprot ID   -
Organism   Pseudomonas sp. SC3(2021)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1194192..1206273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5Y35_RS05245 - 1195816..1197405 (-) 1590 WP_123470763.1 AAA family ATPase -
  K5Y35_RS05250 aroB 1197416..1198516 (-) 1101 WP_123470762.1 3-dehydroquinate synthase -
  K5Y35_RS05255 aroK 1198669..1199187 (-) 519 WP_045122260.1 shikimate kinase AroK -
  K5Y35_RS05260 pilQ 1199192..1201273 (-) 2082 WP_222833824.1 type IV pilus secretin PilQ Machinery gene
  K5Y35_RS05265 - 1201287..1201814 (-) 528 WP_222833825.1 pilus assembly protein PilP -
  K5Y35_RS05270 pilO 1201811..1202434 (-) 624 WP_123470756.1 type 4a pilus biogenesis protein PilO -
  K5Y35_RS05275 - 1202431..1202997 (-) 567 WP_123470754.1 PilN domain-containing protein -
  K5Y35_RS05280 pilM 1202997..1204061 (-) 1065 WP_123470753.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 75031.44 Da        Isoelectric Point: 5.5771

>NTDB_id=600340 K5Y35_RS05260 WP_222833824.1 1199192..1201273(-) (pilQ) [Pseudomonas sp. SC3(2021)]
MNRIFSTLGFSLWIALMSPMVLAANLKALDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIAVDLPGVASQLASKNFD
LGSGNARTATVVEAKDRTRLIVSLTQLAPYNTRVEGNTLYVVVGQGAPVAAPRPAAAVPRATTVAAAPAKAFVAKSRAIR
GVDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAGVSGDRTVVTVEPGG
TYEYSTFQTDNKLTVSVRPMTVDDLQKRNADRNSYVGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVS
NGNSTTIGTPGSTSTNSPFVDMGTVGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIP
YQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFS
NTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=600340 K5Y35_RS05260 WP_222833824.1 1199192..1201273(-) (pilQ) [Pseudomonas sp. SC3(2021)]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGATGTCGCCGATGGTACTCGCTGCCAACCTGAA
GGCGCTGGACGTGGCGGCATTGCCGGGCGACCGCGTCGAACTGAAGTTGTCGTTCGACGGCCCGCCACCGCAGCCCAAGG
GCTACACCACCGAGTCGCCGGCGCGCATCGCTGTGGATTTGCCCGGTGTCGCCAGTCAGTTGGCGAGCAAAAATTTCGAT
CTGGGCAGTGGCAATGCGCGCACTGCCACGGTGGTCGAGGCCAAGGACCGGACGCGGCTGATCGTCAGCCTCACGCAGTT
GGCGCCCTATAACACGCGAGTGGAGGGCAATACTCTGTACGTGGTGGTCGGCCAGGGCGCGCCGGTTGCGGCCCCCCGTC
CCGCAGCCGCCGTACCCCGTGCGACGACCGTCGCTGCTGCTCCGGCCAAGGCCTTCGTGGCGAAAAGCCGAGCGATTCGC
GGTGTCGATTTCCAGCGCGGTACGGCGGGCGAGGGCAATGTGGTCATCGACCTGTCCGACCCGACCATTGCCCCGGATAT
CCAGGAGCATGACGGCAAGATCATCCTCAGCTTCGCCCGTACTCAGTTGCCGGAGAAACTGCGAGTACGGCTCGACGTCA
AGGATTTCGCCACGCCGGTGCAGTTCGTCAATGCCGGGGTGAGCGGTGATCGCACGGTGGTCACCGTCGAACCCGGCGGC
ACTTACGAGTATTCGACTTTCCAGACCGACAACAAACTGACCGTCAGCGTCCGCCCGATGACGGTCGACGATCTGCAAAA
GCGTAATGCCGACCGCAACAGCTACGTCGGCGAAAAGCTCTCGTTGAACTTCCAGGACATCGACGTGCGCTCGGTACTGC
AATTGATCGCCGACTTCACCAACCTCAATCTGGTCGCCAGCGACACAGTGCAGGGCGGTATCACCTTGCGCCTGCAGAAC
GTGCCGTGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAGGGGCTGGATAAACGCAAGATCGGCAATGTGCTGCTGGT
GGCACCCGCCGATGAAATCGCCGCCCGTGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATCGCCGAACTGGCGCCGTTGC
GCCGCGAACTGCTGCAAGTGAACTACGCCAAGGCGGCCGACATCGCCAAACTGTTCCAGTCGGTGACCAGCGCCGAAGCG
AAAGTCGATGAGCGTGGCTCGATCACGGTCGATGAGCGCACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
CGAACTGCGGCGGATTGTGGCGCAGCTGGATATCCCGGTACGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACG
TCGATTACGACAAGAGCCTGGGCGTGCGCTGGGGCGGTTCGATCCAGAACAAGGGCAACTGGAACACGTCCGGGGTCAGT
AACGGCAACTCCACCACCATTGGCACGCCGGGCAGCACCAGCACCAACTCGCCGTTCGTCGACATGGGCACGGTCGGCAA
CACGTCCGGGATCGGCATTGCGTTCATCACCGACAATGTATTGCTCGATCTTGAGCTGACAGCGATGGAAAAAACCGGCA
ACGGCGAAATTGTCTCCCAGCCGAAGGTGGTCACCTCCGACAAGGAGACCGCGAAGATCCTCAAGGGCACCGAGATTCCG
TACCAGGAAGCCAGCTCCAGCGGGGCGACGTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAGGTGACGCCGCAGAT
CACGCCCGACAACCGCATCATCATGGAAGTGAAGGTCACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGGATGTAC
CGCCGATCAAGAAAAACGAGGTCAACGCCAAAGTACTGGTGAATGACGGCGAAACCATCGTGATTGGGGGCGTTTTCTCA
AATACTCAAAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGT
GGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTT
GA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(37-121)

Bacterial type II and III secretion system protein (Secretin)

(528-684)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.067

100

0.745
  comQ Acinetobacter baylyi ADP1

40.922

100

0.41
  pilQ Acinetobacter baumannii strain A118

37.816

100

0.41
  pilQ Acinetobacter baumannii D1279779

37.816

100

0.41
  pilQ Legionella pneumophila strain ERS1305867

37.982

97.258

0.369
  pilQ Legionella pneumophila strain Lp02

37.982

97.258

0.369