Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KFZ60_RS01850 Genome accession   NZ_CP098231
Coordinates   411903..413141 (+) Length   412 a.a.
NCBI ID   WP_115978151.1    Uniprot ID   -
Organism   Escherichia coli strain XJ34     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 406903..418141
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFZ60_RS01825 (KFZ60_01820) mrcA 407183..409735 (-) 2553 WP_088375912.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  KFZ60_RS01830 (KFZ60_01825) hofM 409855..410634 (+) 780 WP_001308095.1 DNA utilization protein HofM -
  KFZ60_RS01835 (KFZ60_01830) hofN 410634..411173 (+) 540 WP_001069342.1 DNA utilization protein HofN -
  KFZ60_RS01840 (KFZ60_01835) hofO 411157..411597 (+) 441 WP_047644745.1 DNA utilization protein HofO -
  KFZ60_RS01845 (KFZ60_01840) hofP 411587..411991 (+) 405 WP_028132028.1 DNA utilization protein HofP -
  KFZ60_RS01850 (KFZ60_01845) pilQ 411903..413141 (+) 1239 WP_115978151.1 DNA uptake porin HofQ Machinery gene
  KFZ60_RS01855 (KFZ60_01850) aroK 413542..414063 (+) 522 WP_000818618.1 shikimate kinase AroK -
  KFZ60_RS01860 (KFZ60_01855) aroB 414120..415208 (+) 1089 WP_000439848.1 3-dehydroquinate synthase -
  KFZ60_RS01865 (KFZ60_01860) damX 415300..416586 (+) 1287 WP_000343210.1 cell division protein DamX -
  KFZ60_RS01870 (KFZ60_01865) dam 416693..417529 (+) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44646.18 Da        Isoelectric Point: 5.9488

>NTDB_id=598714 KFZ60_RS01850 WP_115978151.1 411903..413141(+) (pilQ) [Escherichia coli strain XJ34]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNDNIARQEAEQVRAQANLPLENRNIILQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPGFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=598714 KFZ60_RS01850 WP_115978151.1 411903..413141(+) (pilQ) [Escherichia coli strain XJ34]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGCCAGGAGGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATGACAATATCGCCCGTCAGGAAGCGGA
GCAGGTGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTATTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCTGGGGAAAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCGCACTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGTTTGCTGGATCTTGAGCTTTCTGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCTAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTGCCGTTGCTTGGCGACATTCCCGGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(122-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

38.208

100

0.393
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  comE Haemophilus influenzae 86-028NP

36.927

100

0.391
  pilQ Vibrio cholerae strain A1552

37.53

100

0.376
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.53

100

0.376
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364
  pilQ Pseudomonas aeruginosa PAK

34.174

100

0.362