Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   K5Q02_RS06870 Genome accession   NZ_CP081942
Coordinates   1532827..1533891 (+) Length   354 a.a.
NCBI ID   WP_225837674.1    Uniprot ID   -
Organism   Pseudomonas sp. MM211     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1527827..1538891
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5Q02_RS06860 (K5Q02_06835) - 1528760..1530028 (+) 1269 WP_225837670.1 malic enzyme-like NAD(P)-binding protein -
  K5Q02_RS06865 (K5Q02_06840) - 1530207..1532645 (-) 2439 WP_225837672.1 penicillin-binding protein 1A -
  K5Q02_RS06870 (K5Q02_06845) pilM 1532827..1533891 (+) 1065 WP_225837674.1 pilus assembly protein PilM Machinery gene
  K5Q02_RS06875 (K5Q02_06850) - 1533891..1534463 (+) 573 WP_225837676.1 PilN domain-containing protein -
  K5Q02_RS06880 (K5Q02_06855) pilO 1534460..1535083 (+) 624 WP_225837678.1 type 4a pilus biogenesis protein PilO -
  K5Q02_RS06885 (K5Q02_06860) pilP 1535080..1535598 (+) 519 WP_225837680.1 type 4a pilus biogenesis lipoprotein PilP -
  K5Q02_RS06890 (K5Q02_06865) pilQ 1535653..1537794 (+) 2142 WP_225837682.1 type IV pilus secretin PilQ Machinery gene
  K5Q02_RS06895 (K5Q02_06870) aroK 1537798..1538316 (+) 519 WP_225837684.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38056.33 Da        Isoelectric Point: 4.4260

>NTDB_id=598558 K5Q02_RS06870 WP_225837674.1 1532827..1533891(+) (pilM) [Pseudomonas sp. MM211]
MLGLFSKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPANAVVEKNIAELEGVGEALSRLLAKAKTNVRTVAV
AVAGSAVITKTIEMESGLSDDELENQLKLEADQYIPYPLEEVAIDFEVQGTSPRNSDRVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYNLLSEQLGGVHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
DEAGLAKKQGGLPDDYESEVLQPFKDALVQQVSRSLQFFFAAGQYNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=598558 K5Q02_RS06870 WP_225837674.1 1532827..1533891(+) (pilM) [Pseudomonas sp. MM211]
GTGCTAGGGCTCTTCAGTAAGAAAGCGAATACGCTTTTGGGGATCGATATAAGTTCGACCTCCGTCAAGCTCCTCGAATT
GAGTCGCTCAGGAAGCCGCTACAAGGTAGAGGCTTATGCTGTCGAGCCGCTCCCCGCCAATGCTGTCGTCGAGAAAAATA
TCGCCGAGCTCGAGGGTGTCGGTGAGGCGCTGTCGCGCTTGCTGGCCAAGGCGAAGACCAATGTGCGTACGGTGGCAGTC
GCTGTTGCAGGCTCTGCGGTCATCACCAAGACCATTGAGATGGAATCTGGGCTTTCGGATGACGAGTTGGAAAATCAGCT
GAAGTTGGAAGCCGACCAGTACATCCCTTATCCGCTTGAAGAGGTGGCGATCGATTTCGAAGTTCAGGGCACGTCCCCGC
GCAACTCGGATCGCGTTGAAGTGCTGCTGGCTGCCTGCCGCAAGGAAAACGTCGAAGTTCGCGAGGCTGCCCTGGCGTTG
GCTGGGCTGACTGCCAAAGTGGTGGATGTCGAAGCCTATGCCTTGGAGCGTGCCTACAACTTGCTCTCCGAGCAGTTGGG
TGGTGTCCATGATGAGCTGACCGTCGCAGTGGTCGACATTGGCGCGACCATGACCACGCTCAGCGTGCTGCACAACGGGC
GCACCATCTACACCCGCGAACAGCTGTTCGGTGGCCGGCAATTGACTGAAGAAATTCAGCGCCGTTATGGCCTTTCCGTC
GACGAAGCGGGTCTTGCGAAAAAGCAGGGTGGTCTGCCCGACGATTACGAAAGCGAGGTACTGCAGCCGTTCAAGGATGC
ACTTGTGCAGCAGGTCTCCCGTTCGCTGCAATTCTTCTTTGCCGCCGGCCAGTACAACGATGTCGATTACATCCTGTTGG
CGGGCGGGACGGCGTCGATCCCGGATCTTGATCGACTCATTCAGCAGAAAATCGGTACCCAGACGTTGGTCGCCAATCCA
TTCGCAGACATGGCCCTGAGTGGCAAGGTCAATGCGGGTGCGCTGGCCAGTGATGCGCCGGCACTGATGATTGCCTGCGG
CCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.775

100

0.559
  comM Acinetobacter nosocomialis M2

55.775

100

0.559
  comM Acinetobacter baylyi ADP1

52.958

100

0.531
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466