NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	NBY12_RS20550	Genome accession	NZ_CP098223
Coordinates	4293762..4294886 (-)	Length	374 a.a.
NCBI ID	WP_000228564.1	Uniprot ID	B7LRQ2
Organism	Escherichia coli strain Z0117EC0005
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 4288762..4299886

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NBY12_RS20520 (NBY12_20545)	yrdA	4290562..4291116 (+)	555	WP_001286209.1	gamma carbonic anhydrase family protein	-
NBY12_RS20525 (NBY12_20550)	yrdB	4291092..4291349 (-)	258	WP_001070585.1	DUF1488 domain-containing protein	-
NBY12_RS20530 (NBY12_20555)	aroE	4291346..4292164 (-)	819	WP_000432883.1	shikimate dehydrogenase	-
NBY12_RS20535 (NBY12_20560)	tsaC	4292169..4292741 (-)	573	WP_001353637.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
NBY12_RS20540 (NBY12_20565)	yrdD	4292746..4293288 (-)	543	WP_032303882.1	type I DNA topoisomerase	-
NBY12_RS20545 (NBY12_20570)	smg	4293317..4293790 (-)	474	WP_000460672.1	DUF494 family protein Smg	-
NBY12_RS20550 (NBY12_20575)	dprA	4293762..4294886 (-)	1125	WP_000228564.1	DNA-protecting protein DprA	Machinery gene
NBY12_RS20555 (NBY12_20580)	def	4295016..4295525 (+)	510	WP_000114984.1	peptide deformylase	-
NBY12_RS20560 (NBY12_20585)	fmt	4295540..4296487 (+)	948	WP_000004453.1	methionyl-tRNA formyltransferase	-
NBY12_RS20565 (NBY12_20590)	rsmB	4296538..4297827 (+)	1290	WP_032303883.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
NBY12_RS20570 (NBY12_20595)	trkA	4297849..4299225 (+)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
NBY12_RS20575 (NBY12_20600)	mscL	4299355..4299765 (+)	411	WP_000022442.1	large-conductance mechanosensitive channel protein MscL	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40831.76 Da Isoelectric Point: 6.4375

                                >NTDB_id=598537 NBY12_RS20550 WP_000228564.1 4293762..4294886(-) (dprA) [Escherichia coli strain Z0117EC0005]

MVDTEIWLRLMSVSSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPPKSIESSLCWLNQPNHHLISADSE

FYPSQLLATADYPGALFVAGELRALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=598537 NBY12_RS20550 WP_000228564.1 4293762..4294886(-) (dprA) [Escherichia coli strain Z0117EC0005]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCGTCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCCAAAGAGTATTGAGAGCTCACTTTGTTGGTTGAATCAACCAAACCATCATTTAATCTCAGCGGATAGTGAA

TTTTATCCTTCCCAACTTCTGGCGACAGCAGATTACCCCGGTGCGCTGTTTGTTGCTGGAGAACTGCGCGCATTGCATTC

ATTTCAGCTTGCTGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAACGCTGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGAGGAATCGATGGTGTGGCGCATAAAGCGGCCTTGCAGGTGAAT

GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCCCGGCTGGCTACCAGTCTGCT

TGAACATGGCGGAGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCTCTTGCTTACAATTTCCCGCGAAGAAATCGCA

TTATCAGTGGTCTAAGTAAAGGTGTACTTGTGGTGGAAGCTGCTTTGCGCAGTGGTTCGCTGGTGACAGCGCGTTGTGCG

CTTGAACAGGGGCGAGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCGC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterProScan.

DprA winged helix domain (WHD_DprA)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(68-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	B7LRQ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	47.826	98.396	0.471
dprA	Vibrio cholerae O1 biovar El Tor strain E7946	44.324	98.93	0.438
dprA	Vibrio cholerae strain A1552	44.324	98.93	0.438
dprA	Haemophilus influenzae Rd KW20	44.807	90.107	0.404
dprA	Legionella pneumophila strain ERS1305867	44.248	90.642	0.401
dprA	Glaesserella parasuis strain SC1401	44.51	90.107	0.401

Detailed information NTG0598537