Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   NBY25_RS23070 Genome accession   NZ_CP098187
Coordinates   4726642..4727880 (-) Length   412 a.a.
NCBI ID   WP_000816009.1    Uniprot ID   -
Organism   Escherichia coli strain Z0117EC0117     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4721642..4732880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBY25_RS23050 (NBY25_23045) dam 4722254..4723090 (-) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -
  NBY25_RS23055 (NBY25_23050) damX 4723197..4724483 (-) 1287 WP_000343173.1 cell division protein DamX -
  NBY25_RS23060 (NBY25_23055) aroB 4724575..4725663 (-) 1089 WP_000439850.1 3-dehydroquinate synthase -
  NBY25_RS23065 (NBY25_23060) aroK 4725720..4726241 (-) 522 WP_000818618.1 shikimate kinase AroK -
  NBY25_RS23070 (NBY25_23065) comE 4726642..4727880 (-) 1239 WP_000816009.1 DNA uptake porin HofQ Machinery gene
  NBY25_RS23075 (NBY25_23070) hofP 4727792..4728196 (-) 405 WP_001264138.1 DNA utilization protein HofP -
  NBY25_RS23080 (NBY25_23075) hofO 4728186..4728626 (-) 441 WP_001055744.1 DNA utilization protein HofO -
  NBY25_RS23085 (NBY25_23080) hofN 4728610..4729149 (-) 540 WP_001069340.1 DNA utilization protein HofN -
  NBY25_RS23090 (NBY25_23085) hofM 4729149..4729928 (-) 780 WP_001308095.1 DNA utilization protein HofM -
  NBY25_RS23095 (NBY25_23090) mrcA 4730048..4732600 (+) 2553 WP_001298208.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44811.29 Da        Isoelectric Point: 5.9488

>NTDB_id=597538 NBY25_RS23070 WP_000816009.1 4726642..4727880(-) (comE) [Escherichia coli strain Z0117EC0117]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNVARQEVEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQGGGVGQVTTLGSDLSVATATTHIGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=597538 NBY25_RS23070 WP_000816009.1 4726642..4727880(-) (comE) [Escherichia coli strain Z0117EC0117]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTTATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTTTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATTTAACTGACGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGATTG
ATAACGCGCCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATGTCGCCCGCCAGGAAGTGGA
GCAGACGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGTTGGCCGATGCGCAACAAGGTGGTGGCGTTG
GGCAAGTCACTACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATATCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTAGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.385

100

0.396
  pilQ Vibrio campbellii strain DS40M4

38.48

100

0.393
  comE Haemophilus influenzae 86-028NP

37.156

100

0.393
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  pilQ Pseudomonas aeruginosa PAK

35.092

100

0.371
  comE Glaesserella parasuis strain SC1401

35.782

100

0.367