Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   NBY25_RS22565 Genome accession   NZ_CP098187
Coordinates   4644934..4646058 (-) Length   374 a.a.
NCBI ID   WP_000228517.1    Uniprot ID   A0A0H3EME5
Organism   Escherichia coli strain Z0117EC0117     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4639934..4651058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBY25_RS22535 (NBY25_22530) yrdA 4641734..4642288 (+) 555 WP_001286206.1 gamma carbonic anhydrase family protein -
  NBY25_RS22540 (NBY25_22535) yrdB 4642264..4642521 (-) 258 WP_001070563.1 DUF1488 domain-containing protein -
  NBY25_RS22545 (NBY25_22540) aroE 4642518..4643336 (-) 819 WP_000451249.1 shikimate dehydrogenase -
  NBY25_RS22550 (NBY25_22545) tsaC 4643341..4643913 (-) 573 WP_001468260.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  NBY25_RS22555 (NBY25_22550) yrdD 4643918..4644460 (-) 543 WP_001312137.1 type I DNA topoisomerase -
  NBY25_RS22560 (NBY25_22555) smg 4644489..4644962 (-) 474 WP_000460672.1 DUF494 family protein Smg -
  NBY25_RS22565 (NBY25_22560) dprA 4644934..4646058 (-) 1125 WP_000228517.1 DNA-protecting protein DprA Machinery gene
  NBY25_RS22570 (NBY25_22565) def 4646188..4646697 (+) 510 WP_000114984.1 peptide deformylase -
  NBY25_RS22575 (NBY25_22570) fmt 4646712..4647659 (+) 948 WP_000004425.1 methionyl-tRNA formyltransferase -
  NBY25_RS22580 (NBY25_22575) rsmB 4647711..4649000 (+) 1290 WP_000744773.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  NBY25_RS22585 (NBY25_22580) trkA 4649022..4650398 (+) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  NBY25_RS22590 (NBY25_22585) mscL 4650528..4650941 (+) 414 WP_000022450.1 large-conductance mechanosensitive channel protein MscL -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40910.86 Da        Isoelectric Point: 6.0395

>NTDB_id=597534 NBY25_RS22565 WP_000228517.1 4644934..4646058(-) (dprA) [Escherichia coli strain Z0117EC0117]
MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=597534 NBY25_RS22565 WP_000228517.1 4644934..4646058(-) (dprA) [Escherichia coli strain Z0117EC0117]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT
TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGCCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DprA winged helix domain (WHD_DprA)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3EME5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.78

98.663

0.481
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

49.355

82.888

0.409
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Haemophilus influenzae Rd KW20

44.214

90.107

0.398
  dprA Legionella pneumophila strain ERS1305867

44.242

88.235

0.39