Detailed information NTG0596454

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: A237_RS12445
• Genome accession: NZ_CP081457
• Coordinates: 2783221..2784390 (+)
• Length: 389 a.a.
• NCBI ID: WP_017699892.1
• Uniprot ID: A0A3M4KE83
• Organism: Pseudomonas syringae pv. actinidifoliorum ICMP 18803
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2778221..2789390

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A237_RS12430 (A237_012410)	-	2778584..2780917 (+)	2334	WP_017699895.1	ATP-dependent DNA helicase	-
A237_RS12435 (A237_012415)	pgm	2781018..2782664 (+)	1647	WP_017699894.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
A237_RS12440 (A237_012420)	-	2782791..2783066 (-)	276	WP_017699893.1	peptidylprolyl isomerase	-
A237_RS12445 (A237_012425)	pilU	2783221..2784390 (+)	1170	WP_017699892.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
A237_RS12450 (A237_012430)	-	2784603..2786180 (-)	1578	WP_020341396.1	ABC transporter ATP-binding protein	-
A237_RS12455 (A237_012435)	-	2786182..2787204 (-)	1023	WP_005618488.1	ABC transporter permease	-
A237_RS12460 (A237_012440)	-	2787204..2788268 (-)	1065	WP_011104338.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42701.87 Da
• Isoelectric Point: 5.8676

>NTDB_id=596454 A237_RS12445 WP_017699892.1 2783221..2784390(+) (pilU) [Pseudomonas syringae pv. actinidifoliorum ICMP 18803]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVVAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVKTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSENGMTSLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=596454 A237_RS12445 WP_017699892.1 2783221..2784390(+) (pilU) [Pseudomonas syringae pv. actinidifoliorum ICMP 18803]
ATGGATTTCCCGGCGTTGTTGAAGATTCTGGCCAGTCAGGACGGATCTGACCTTTATCTTTCTACCGGAGCACCGCCGTG
TGCCAAGTTCAACGGCGTTCTCAAACCGCTGGGCAGCGAAACGCTCAAGCCCGGCGAAGTGGCAGTGGTTGCTCAAGGGC
TGATGGACGAGGAGCAGAAGCTTGAGTTTCTCCGGGAACTGGAGATGAACCTCGCTGTGTCGCTGGCCGGCATTGGTCGG
TTCCGGATCAATATCTTCATGCAGCGCAACGAAGTGTCCATTGTTGCGCGTAACATCAAGCTTGATATTCCGCGCTTTGA
GGACCTGTTCCTGCCACCGGTATTGCTCGACGTGATCATGGAAAAACACGGTCTGGTGCTGTTTGTCGGCGCCACCGGCT
CGGGCAAATCGACCTCGCTGGCGGCACTGATCGATTACCGTAACCGCAATGCCAGCGGGCATATCATCACCATTGAAGAC
CCGGTCGAATTTATCCATCGGCACAAGAAATCCATCGTCAATCAGCGGGAGGTCGGCGTCGACACCCGCAGCTTTCGAGC
GGCGCTGAAAAACACCCTGCGTCAGGCCCCGGATGTGATTCTGATCGGCGAGATCCGCGATCGCGAAACGATGGAGCACG
CGCTGGCATTCGCCGACACCGGCCACTTGGCGATTTCGACCCTGCACGCCAATAACGCCAACCAGGCGTTGGACCGAATC
ATCAACTTTTTCCCGGAAGAGCGACGTGCGCAGCTGTTGCACGACCTGGGCAACAACCTCAAGGCGTTCGTGTCTCAGCG
GCTGGTAAAGACGCCTGATGGAAAACGGCGCGCGGCGGTGGAAGTGATGATGGGCACGCCGACCATCCGCGATCTGATCC
AGCGTAATGAGCTGACTGAACTCAAGGGCATCATGGAAAAGTCCGGGAGCCTCGGGATGCAGACGTTCGATACCGCGCTG
TTCAACCTGGCCGTCGAAGGTGCGATTAGCGAGGAAGAGGCGTTGAAGAATGCCGACTCGCAAAACAACGTGCGCCTTCG
CCTCAAGCTGCACAGCGAGAACGGAATGACCAGCCTGACCACGCCGCCCCCGGCACCGACCGGCAGCAGCACGGCCAGCA
CCGCCGAGTGGGGCCTTGTCGACGATGACGAGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-248)

3D structure

• AlphaFold DB: A0A3M4KE83

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.072	99.486	0.558
pilU	Acinetobacter baylyi ADP1	51.831	91.26	0.473
pilU	Vibrio cholerae strain A1552	51.429	89.974	0.463
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.426	98.458	0.388
pilT	Pseudomonas aeruginosa PAK	41.399	88.175	0.365