Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   M9O75_RS03855 Genome accession   NZ_CP097724
Coordinates   794671..796164 (+) Length   497 a.a.
NCBI ID   WP_001468188.1    Uniprot ID   -
Organism   Escherichia coli strain MS1494     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 789671..801164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9O75_RS03835 (M9O75_03835) pilD 790319..791128 (+) 810 WP_000895866.1 prepilin peptidase PppA Machinery gene
  M9O75_RS03840 (M9O75_03840) gspS2 791194..791604 (+) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  M9O75_RS03845 (M9O75_03845) gspC 791622..792581 (+) 960 WP_000135085.1 type II secretion system protein GspC -
  M9O75_RS03850 (M9O75_03850) gspD 792611..794671 (+) 2061 WP_001468189.1 type II secretion system secretin GspD -
  M9O75_RS03855 (M9O75_03855) pilB/pilB1 794671..796164 (+) 1494 WP_001468188.1 type II secretion system ATPase GspE Machinery gene
  M9O75_RS03860 (M9O75_03860) gspF 796164..797387 (+) 1224 WP_001535576.1 type II secretion system inner membrane protein GspF -
  M9O75_RS03865 (M9O75_03865) gspG 797404..797859 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  M9O75_RS03870 (M9O75_03870) gspH 797863..798426 (+) 564 WP_001468411.1 type II secretion system minor pseudopilin GspH -
  M9O75_RS03875 (M9O75_03875) gspI 798423..798794 (+) 372 WP_001468410.1 type II secretion system minor pseudopilin GspI -
  M9O75_RS03880 (M9O75_03880) gspJ 798791..799396 (+) 606 WP_001468409.1 type II secretion system minor pseudopilin GspJ -
  M9O75_RS03885 (M9O75_03885) gspK 799393..800370 (+) 978 WP_001468408.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55154.29 Da        Isoelectric Point: 5.9081

>NTDB_id=596330 M9O75_RS03855 WP_001468188.1 794671..796164(+) (pilB/pilB1) [Escherichia coli strain MS1494]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCSDCRQAAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=596330 M9O75_RS03855 WP_001468188.1 794671..796164(+) (pilB/pilB1) [Escherichia coli strain MS1494]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTATAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTGCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAACGGGGGCTGA
ACGCGCCCTTTACGCTGAGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGATTTTTTTACCCTCGCCGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCAATCATCAAACTGATCAACGCCATGTTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTGTCGCGTATCAAGGTGATGGCGCGGCTGGACATTGCCGAAAAGCGCGTGCC
GCAGGATGGACGTATTGCGCTGTTGCTGGGCGGCCGGGCGATTGACGTGCGTGTATCAACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCTCGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACATTCGCCCGTGGCCTGCGCGCAATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTCCGCACGCTGTGCTCCGACTGCCGTCAGGCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCACAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGGACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGACAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.741

100

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.765

85.513

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

47.859

79.879

0.382
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio campbellii strain DS40M4

46.97

79.678

0.374
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilB Acinetobacter baylyi ADP1

46.667

78.471

0.366
  pilF Thermus thermophilus HB27

45

80.483

0.362