Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K3819_RS16150 Genome accession   NZ_CP081200
Coordinates   3592791..3593903 (-) Length   370 a.a.
NCBI ID   WP_221397453.1    Uniprot ID   -
Organism   Pseudomonas sp. 3-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3587791..3598903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3819_RS16145 (K3819_16145) - 3588709..3592572 (-) 3864 WP_221397452.1 hypothetical protein -
  K3819_RS16150 (K3819_16150) pilU 3592791..3593903 (-) 1113 WP_221397453.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K3819_RS16155 (K3819_16155) - 3594032..3596569 (+) 2538 WP_221397454.1 PAS domain-containing protein -
  K3819_RS16160 (K3819_16160) - 3596620..3596895 (+) 276 WP_007952044.1 peptidylprolyl isomerase -
  K3819_RS16165 (K3819_16165) - 3597020..3597952 (-) 933 WP_221397455.1 sugar kinase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41154.15 Da        Isoelectric Point: 6.7879

>NTDB_id=595597 K3819_RS16150 WP_221397453.1 3592791..3593903(-) (pilU) [Pseudomonas sp. 3-2]
MEIDALLKLLASRHGSDLFLSTGAPPSARFDGVLTPLSDQPFKPGEVSSVAAFLMDAEQRREFDRDLEMNLAISRTGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPPVLLETVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFAETGHLVLSTLHAVNASQALDRA
VNLFPEERRPQLMHALGNNLKALVSQRLVRTVDGQRRAAVEVMLGSPTIADLIKNNQLDDLKVIMEKSGELGMRTFDSAL
HQLVIEGAISENEALKNADSVNNLRLLLKLNEQNVRADKTSSESWGLLDE

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=595597 K3819_RS16150 WP_221397453.1 3592791..3593903(-) (pilU) [Pseudomonas sp. 3-2]
ATGGAAATCGACGCACTGCTCAAACTGCTGGCGAGCCGTCACGGTTCGGATCTGTTTCTTTCCACCGGTGCACCACCCAG
TGCGCGGTTCGACGGGGTACTGACGCCCTTGAGCGACCAGCCCTTCAAACCCGGCGAGGTGAGCAGTGTCGCCGCCTTCC
TGATGGACGCCGAGCAGCGCCGTGAGTTCGACCGGGATCTGGAAATGAACCTGGCGATTTCTCGAACGGGTATCGGCCGC
TTTCGGGTCAATATTTTCAAACAACGCAACGACGTGTCGATTGTTATCCGTAACGTCAAACTCGACATTCCGCGATTCGA
AGACCTCAAATTGCCGCCAGTGCTGCTCGAAACGGTGATGCTCAAGCAGGGGTTGATTCTGTTCGTGGGCGCTACCGACT
CCGGCAAGTCTACCTCCCTGGCGGCGCTGATCGATCATCGCAATCGCCACAGCAGCGGGCACATCATCACCATTGAGGAT
CCGGTGGAGTATATCCATCGACACAAGAAGTCGATCATCAATCAGCGCGAAGTCGGGGTCGACACACGTAGCTTTCATGC
GGCGCTCAAGAACACCTTGCGCCAGGCTCCGGACGTGGTGCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGCATG
CATTGGCGTTCGCCGAAACCGGGCATCTGGTTCTCTCCACATTGCATGCTGTCAACGCCAGTCAGGCGCTGGATCGAGCC
GTCAATTTATTTCCCGAGGAGCGGCGTCCTCAACTGATGCACGCCTTGGGCAACAACCTCAAGGCTCTCGTGTCCCAGCG
TCTGGTACGAACCGTCGATGGGCAGCGGCGCGCGGCCGTGGAAGTGATGCTGGGATCGCCGACGATTGCCGACCTGATCA
AGAACAACCAGTTGGACGATCTGAAAGTCATCATGGAAAAGTCCGGAGAACTTGGTATGCGCACCTTTGACAGCGCCCTG
CATCAACTGGTGATTGAAGGTGCAATCAGTGAAAACGAAGCGTTGAAAAATGCTGACTCGGTCAATAACTTGCGTCTTTT
ACTGAAGTTGAATGAGCAGAATGTACGTGCCGACAAAACGTCTTCGGAAAGTTGGGGGCTGCTTGATGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(69-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.261

99.459

0.53
  pilU Acinetobacter baylyi ADP1

52.113

95.946

0.5
  pilU Vibrio cholerae strain A1552

50

94.595

0.473
  pilT Pseudomonas stutzeri DSM 10701

39.535

92.973

0.368
  pilT Pseudomonas aeruginosa PAK

38.953

92.973

0.362