Detailed information NTG0595395

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: K4H28_RS13745
• Genome accession: NZ_CP081150
• Coordinates: 2942657..2943841 (+)
• Length: 394 a.a.
• NCBI ID: WP_221005717.1
• Uniprot ID: -
• Organism: Deefgea tanakiae strain D25
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2937657..2948841

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K4H28_RS13725 (K4H28_13725)	-	2937865..2938545 (-)	681	WP_221005713.1	metallophosphoesterase	-
K4H28_RS13730 (K4H28_13730)	-	2938745..2939998 (+)	1254	WP_221005714.1	Hsp70 family protein	-
K4H28_RS13735 (K4H28_13735)	-	2940290..2942170 (+)	1881	WP_221005715.1	DEAD/DEAH box helicase	-
K4H28_RS13740 (K4H28_13740)	-	2942242..2942583 (+)	342	WP_221005716.1	antibiotic biosynthesis monooxygenase	-
K4H28_RS13745 (K4H28_13745)	pilU	2942657..2943841 (+)	1185	WP_221005717.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
K4H28_RS13750 (K4H28_13750)	-	2943896..2945200 (-)	1305	WP_221005718.1	hemolysin family protein	-
K4H28_RS13755 (K4H28_13755)	cyoE	2945411..2946313 (-)	903	WP_221005719.1	heme o synthase	-
K4H28_RS13760 (K4H28_13760)	-	2946310..2947320 (-)	1011	WP_221005720.1	heme A synthase	-
K4H28_RS13765 (K4H28_13765)	-	2947356..2947808 (-)	453	WP_221005721.1	hypothetical protein	-
K4H28_RS13770 (K4H28_13770)	-	2947792..2948463 (-)	672	WP_221005722.1	SURF1 family protein	-
K4H28_RS13775 (K4H28_13775)	-	2948507..2948695 (+)	189	WP_221005723.1	DUF2909 family protein	-

Sequence

Protein

• Length: 394 a.a.
• Molecular weight: 44108.70 Da
• Isoelectric Point: 6.3938

>NTDB_id=595395 K4H28_RS13745 WP_221005717.1 2942657..2943841(+) (pilU) [Deefgea tanakiae strain D25]
MSPEQANKTMNDLLKMMIQHKASDLFIADDFPPAMKINGKMTPVGSQKLNGTASKALAYSIMRPDQQAEFENEFEANFAI
SPEGIGRFRVNVFMQQEKVGMVLRTITSKIPNFDEMGLPKILKEVMMQKRGLVLLVGGTGSGKSTTMAAMLDYRNEHSHG
HIITIEDPIEYVHKNKNCLVTHREVGRDTKGWFAALKNTLRQAPDVILIGEIRDRETMEYAMNFAETGHLCMGTLHANSS
NQALERIVNFFPEERHPQLYMDLSMNMRGIISQRLVPTPDGKGRSAAIEILLNTPLVADLIFKGDVGGIKSVMAKSKELG
MQTFDMALFDLYEAGKIGMEEALKNADSLNELRLNIKLNSQHHRKEEHSSGLDHLSLEAHAEPEVEEEAVEKNK

Nucleotide

• Length: 1185 bp

>NTDB_id=595395 K4H28_RS13745 WP_221005717.1 2942657..2943841(+) (pilU) [Deefgea tanakiae strain D25]
ATGAGCCCCGAACAAGCCAATAAAACGATGAACGACTTGCTGAAAATGATGATTCAGCACAAAGCGTCTGACTTATTCAT
CGCCGATGACTTCCCGCCTGCGATGAAAATCAACGGCAAAATGACGCCCGTCGGCTCACAAAAACTCAATGGCACTGCCA
GCAAGGCGCTGGCCTACAGCATTATGCGCCCCGATCAGCAGGCTGAATTCGAAAACGAATTTGAAGCCAACTTTGCGATC
AGCCCCGAAGGTATTGGTCGATTTCGCGTGAATGTGTTTATGCAGCAAGAAAAAGTCGGCATGGTGCTGCGCACGATCAC
CAGCAAGATTCCGAATTTCGATGAAATGGGCCTACCGAAAATCCTGAAAGAAGTAATGATGCAAAAACGTGGCCTCGTAC
TGCTGGTCGGCGGTACCGGCTCAGGCAAATCGACGACGATGGCCGCGATGCTCGATTACCGCAACGAGCACAGCCACGGC
CATATCATCACCATCGAAGATCCGATTGAATACGTACACAAAAACAAGAATTGCCTTGTTACGCACCGCGAAGTCGGCCG
CGATACCAAGGGCTGGTTTGCCGCGCTGAAAAACACGCTACGCCAGGCGCCCGATGTGATTTTGATTGGCGAGATACGGG
ATAGAGAAACGATGGAATATGCGATGAATTTCGCCGAAACCGGCCATCTTTGCATGGGCACGCTGCACGCCAATAGCTCG
AATCAAGCGCTGGAACGCATCGTCAATTTCTTCCCCGAAGAGCGCCATCCTCAGCTCTATATGGATTTGTCGATGAATAT
GCGCGGCATCATCTCGCAGCGCCTCGTGCCGACGCCCGACGGCAAGGGCCGCAGCGCCGCGATTGAAATTCTGCTCAATA
CACCGCTGGTGGCCGATTTGATTTTCAAAGGCGACGTCGGCGGCATCAAATCGGTGATGGCCAAATCCAAAGAGCTGGGC
ATGCAAACCTTCGATATGGCGCTGTTTGACCTCTACGAAGCAGGAAAAATCGGCATGGAAGAAGCACTAAAGAATGCCGA
TTCGCTGAATGAACTGCGCCTAAATATTAAGCTCAACAGCCAACATCACCGCAAAGAAGAACACAGCAGCGGGCTTGATC
ACCTCTCCCTCGAAGCGCATGCCGAGCCGGAAGTGGAAGAAGAGGCTGTAGAAAAAAATAAGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-277)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.307	88.579	0.508
pilU	Acinetobacter baylyi ADP1	56.374	89.594	0.505
pilU	Vibrio cholerae strain A1552	52.486	91.878	0.482
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.6	95.178	0.396
pilT	Pseudomonas aeruginosa PAK	43.323	85.533	0.371