Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K2O51_RS15165 Genome accession   NZ_CP080767
Coordinates   576151..576669 (+) Length   172 a.a.
NCBI ID   WP_220631300.1    Uniprot ID   -
Organism   Cupriavidus pinatubonensis strain HN-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 571151..581669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K2O51_RS15140 (K2O51_15140) recX 571206..571676 (+) 471 WP_011296717.1 recombination regulator RecX -
  K2O51_RS15145 (K2O51_15145) - 571968..572642 (+) 675 WP_220631299.1 DUF2889 domain-containing protein -
  K2O51_RS15150 (K2O51_15150) sucC 572730..573899 (+) 1170 WP_011296719.1 ADP-forming succinate--CoA ligase subunit beta -
  K2O51_RS15155 (K2O51_15155) sucD 573989..574870 (+) 882 WP_011296720.1 succinate--CoA ligase subunit alpha -
  K2O51_RS15160 (K2O51_15160) - 575142..575837 (+) 696 WP_011296721.1 TerC family protein -
  K2O51_RS15165 (K2O51_15165) pilA 576151..576669 (+) 519 WP_220631300.1 pilin Machinery gene
  K2O51_RS15170 (K2O51_15170) - 576761..578557 (+) 1797 WP_220631301.1 Wzy polymerase domain-containing protein -
  K2O51_RS15175 (K2O51_15175) - 578568..579098 (-) 531 WP_220631302.1 YMGG-like glycine zipper-containing protein -
  K2O51_RS15180 (K2O51_15180) - 579095..579583 (-) 489 WP_220631303.1 hypothetical protein -
  K2O51_RS15185 (K2O51_15185) - 579895..580461 (-) 567 WP_166486978.1 cold shock domain-containing protein -

Sequence

Protein


Download         Length: 172 a.a.        Molecular weight: 17232.81 Da        Isoelectric Point: 10.0092

>NTDB_id=594831 K2O51_RS15165 WP_220631300.1 576151..576669(+) (pilA) [Cupriavidus pinatubonensis strain HN-2]
MQRVQQIKKLGRRVQKGFTLIELMIVVAIIGILAAIAIPAYQDYLVRSRVSEGLSLAESAKTSVTENAANGVAFASGWTA
PSATQNVTSVGITAATGEITITYPARAGGGTLVLVPSSAAANLAVGTVPTGGSVTWECFSAAKAASTLPTAGPAPTAAGT
LAGNFAPAACRT

Nucleotide


Download         Length: 519 bp        

>NTDB_id=594831 K2O51_RS15165 WP_220631300.1 576151..576669(+) (pilA) [Cupriavidus pinatubonensis strain HN-2]
ATGCAACGGGTACAACAAATCAAGAAGCTGGGCCGTCGGGTTCAGAAGGGCTTTACGCTGATCGAACTGATGATCGTGGT
GGCGATTATTGGTATTTTGGCGGCGATTGCTATTCCTGCTTATCAAGATTATCTGGTTCGATCGCGTGTCAGCGAAGGCC
TGTCGCTTGCGGAATCGGCAAAGACGTCAGTTACTGAAAATGCCGCAAACGGGGTGGCTTTTGCTAGTGGTTGGACCGCA
CCTTCGGCTACTCAAAACGTTACGAGTGTTGGTATCACCGCTGCTACCGGCGAAATCACGATCACTTATCCTGCTCGTGC
AGGTGGCGGCACGCTGGTGCTCGTTCCTTCCTCAGCAGCAGCTAACTTGGCCGTTGGCACCGTTCCCACTGGCGGGTCAG
TGACGTGGGAATGCTTCTCGGCTGCTAAGGCCGCTTCCACGTTGCCGACTGCTGGCCCGGCCCCTACCGCAGCAGGTACG
CTGGCGGGCAATTTCGCTCCGGCAGCCTGCCGTACCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(46-171)

Prokaryotic N-terminal methylation motif (N_methyl)

(12-37)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

51.149

100

0.517
  pilA2 Legionella pneumophila str. Paris

50.617

94.186

0.477
  pilA2 Legionella pneumophila strain ERS1305867

50

94.186

0.471
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.824

100

0.424
  comP Acinetobacter baylyi ADP1

50.388

75

0.378