Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A9R16_RS12620 Genome accession   NZ_CP080624
Coordinates   2685246..2686973 (+) Length   575 a.a.
NCBI ID   WP_176715911.1    Uniprot ID   -
Organism   Acidiferrobacter thiooxydans strain ZJ     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2680246..2691973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9R16_RS12595 (A9R16_012750) parE 2680417..2682309 (-) 1893 WP_065971304.1 DNA topoisomerase IV subunit B -
  A9R16_RS12600 (A9R16_012755) - 2682309..2682890 (-) 582 WP_065971305.1 DUF3501 family protein -
  A9R16_RS12605 (A9R16_012760) - 2682913..2683209 (-) 297 WP_065971306.1 encapsulin-associated ferritin-like protein -
  A9R16_RS12610 (A9R16_012765) - 2683243..2684592 (-) 1350 WP_065971307.1 heterodisulfide reductase-related iron-sulfur binding cluster -
  A9R16_RS12615 (A9R16_012770) - 2684665..2685084 (-) 420 WP_110136173.1 rubrerythrin family protein -
  A9R16_RS12620 (A9R16_012775) pilB 2685246..2686973 (+) 1728 WP_176715911.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A9R16_RS12625 (A9R16_012780) - 2687244..2688110 (+) 867 WP_065968815.1 prepilin peptidase -
  A9R16_RS12630 (A9R16_012785) coaE 2688103..2688705 (+) 603 WP_065968816.1 dephospho-CoA kinase -
  A9R16_RS12635 (A9R16_012790) - 2688722..2688976 (-) 255 WP_065968817.1 hypothetical protein -
  A9R16_RS12640 (A9R16_012795) - 2688961..2690031 (-) 1071 WP_065968854.1 malate dehydrogenase -
  A9R16_RS12645 (A9R16_012800) - 2690160..2690567 (-) 408 WP_218060340.1 roadblock/LC7 domain-containing protein -
  A9R16_RS12650 (A9R16_012805) - 2690626..2691036 (-) 411 WP_065968819.1 hypothetical protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62548.07 Da        Isoelectric Point: 6.0638

>NTDB_id=594411 A9R16_RS12620 WP_176715911.1 2685246..2686973(+) (pilB) [Acidiferrobacter thiooxydans strain ZJ]
MATVHPMVNDGVLLTGLAARLVRDQLLTPAEVERLNAESQTNKVSFVTRLVESKKLDALTIAKAASEEFGIPLLDISAFD
SETFPIKLVDAKLIRRHRVLPIFKRGNRLFVAVADPTNLTALDEIKFNTGLLPEPLLVEEGKLGQLIDRNLSDPGDSLAD
MAVSESLEGIDAGGDGAEPADAVAESDINDKPIVRFVNKILLDAINRGASDIHIEPYERTYRVRFRTDGVLQEVAAPPIA
LAARIAARVKILARLDISERRIPQDGRMKIRVSKTRAIDFRVSTLPTLFGEKVVMRLLDPASASLGVDKLGFESDQKAIY
LEAIERPYGMVLVTGPTGSGKTVTLYTALNILNTPDRNISTAEDPVEINLAGINQVNINERAKLTFATALRAFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVISTLHTNDAPQTLGRLVNMGVPPFNIASAVNLIIAQRLARRLCVHCRKPLELPEP
VLRRAGFRAEDIPGLKIFGPGGCDQCNGGYKGRVGIYQVMPVSPAIGQIIMNGGNANDIAEQAQKDGVSDLRQSGLKKVR
DGITSLEEVERVTNE

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=594411 A9R16_RS12620 WP_176715911.1 2685246..2686973(+) (pilB) [Acidiferrobacter thiooxydans strain ZJ]
ATGGCGACAGTCCATCCGATGGTAAATGACGGCGTGTTGTTGACAGGGCTTGCGGCGCGCCTCGTGCGCGACCAGCTGTT
GACGCCGGCTGAGGTCGAGCGCCTGAATGCCGAATCGCAGACCAATAAGGTCTCATTCGTGACGCGGCTCGTGGAGAGCA
AGAAGCTCGACGCCCTGACCATCGCCAAGGCCGCCTCCGAGGAATTCGGTATCCCGCTACTTGACATCAGCGCCTTCGAC
AGCGAGACCTTTCCGATCAAGCTCGTCGACGCCAAACTCATAAGGCGCCACCGCGTGCTGCCGATCTTCAAGCGCGGGAA
TCGCCTGTTCGTGGCCGTGGCCGATCCCACCAATCTGACGGCCTTAGACGAGATCAAGTTCAACACAGGACTGCTCCCCG
AGCCCCTGCTCGTCGAAGAAGGTAAGCTCGGACAACTCATAGACCGCAATCTCAGCGACCCCGGCGATTCACTGGCCGAC
ATGGCGGTAAGCGAATCCCTGGAGGGCATCGATGCGGGCGGGGACGGCGCCGAACCCGCCGATGCCGTCGCCGAAAGTGA
CATCAATGACAAGCCCATCGTCCGCTTCGTCAACAAGATCCTGCTCGACGCCATCAACCGTGGGGCCTCGGACATCCACA
TAGAGCCCTACGAGCGGACTTACCGGGTCCGGTTCCGGACCGACGGTGTCTTGCAGGAGGTCGCCGCCCCGCCGATCGCG
CTCGCGGCGCGCATCGCCGCGCGCGTCAAAATTCTGGCGCGACTCGACATATCCGAGCGCCGCATTCCGCAGGACGGACG
CATGAAGATCCGTGTATCAAAGACGCGCGCGATCGATTTCCGGGTGAGTACCCTGCCGACGCTGTTTGGCGAAAAGGTCG
TGATGCGTCTGCTCGATCCGGCATCGGCCTCGCTGGGCGTCGACAAGCTCGGGTTCGAGTCCGACCAGAAGGCCATCTAC
CTGGAGGCAATCGAGCGGCCCTATGGCATGGTCCTTGTCACCGGACCGACCGGCAGCGGCAAGACCGTAACCCTGTATAC
CGCACTCAATATCCTAAACACCCCGGATCGCAATATTTCGACCGCCGAAGACCCGGTCGAGATCAATCTTGCCGGCATCA
ATCAGGTCAACATCAACGAACGCGCCAAGCTGACCTTCGCCACCGCACTGCGCGCCTTTCTGCGCCAGGACCCCGATATC
ATCATGGTCGGCGAGATCCGCGATCTCGAGACCGCAGAGATCGCGATCAAGGCCGCGCAGACCGGACATATGGTGATCTC
GACTTTGCATACGAATGATGCGCCCCAGACCCTCGGGCGGCTCGTGAACATGGGCGTGCCCCCCTTCAATATCGCCTCGG
CGGTCAATCTCATCATCGCCCAGCGCCTGGCGCGGCGACTGTGCGTGCACTGTCGCAAACCCCTGGAACTCCCGGAACCG
GTCCTGCGCCGGGCGGGCTTTCGCGCGGAAGACATCCCCGGCCTGAAGATCTTCGGCCCAGGGGGCTGCGACCAATGCAA
CGGCGGCTACAAGGGGCGCGTCGGCATCTATCAGGTCATGCCGGTAAGCCCGGCGATCGGCCAGATCATCATGAACGGCG
GCAACGCCAACGACATCGCCGAGCAGGCGCAAAAAGACGGGGTCTCGGACCTGCGTCAATCGGGCCTGAAAAAGGTGCGC
GACGGCATCACGAGCCTCGAGGAAGTGGAGCGCGTGACCAACGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-154)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.773

97.913

0.546
  pilB Acinetobacter baumannii D1279779

54.657

98.957

0.541
  pilB Acinetobacter baylyi ADP1

53.805

98.261

0.529
  pilF Neisseria gonorrhoeae MS11

50

98.783

0.494
  pilB Vibrio cholerae strain A1552

47.965

98.261

0.471
  pilB Vibrio parahaemolyticus RIMD 2210633

48.05

98.087

0.471
  pilB Vibrio campbellii strain DS40M4

46.82

98.435

0.461
  pilF Thermus thermophilus HB27

40.642

97.565

0.397
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.395

91.478

0.388