Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K1Y48_RS07110 Genome accession   NZ_CP080536
Coordinates   1417240..1418949 (+) Length   569 a.a.
NCBI ID   WP_215849997.1    Uniprot ID   -
Organism   Acidithiobacillus ferriphilus strain GT2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1412240..1423949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K1Y48_RS07085 (K1Y48_07110) xth 1412830..1413603 (+) 774 WP_215850000.1 exodeoxyribonuclease III -
  K1Y48_RS07090 (K1Y48_07115) hemH 1413600..1414622 (+) 1023 WP_215849999.1 ferrochelatase -
  K1Y48_RS07095 (K1Y48_07120) - 1414674..1415162 (+) 489 WP_064218850.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  K1Y48_RS07100 (K1Y48_07125) - 1415230..1416393 (+) 1164 WP_226817620.1 anhydro-N-acetylmuramic acid kinase -
  K1Y48_RS07105 (K1Y48_07130) aroE 1416371..1417270 (+) 900 WP_064218849.1 shikimate dehydrogenase -
  K1Y48_RS07110 (K1Y48_07135) pilB 1417240..1418949 (+) 1710 WP_215849997.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K1Y48_RS07115 (K1Y48_07140) - 1419075..1420673 (+) 1599 WP_229065889.1 two-component system sensor histidine kinase NtrB -
  K1Y48_RS07120 (K1Y48_07145) pilR 1420670..1422028 (+) 1359 WP_229065890.1 sigma-54-dependent transcriptional regulator Regulator
  K1Y48_RS07125 (K1Y48_07150) - 1422025..1422825 (+) 801 WP_064220377.1 prepilin peptidase -
  K1Y48_RS07130 (K1Y48_07155) coaE 1422826..1423428 (+) 603 WP_215849805.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62827.54 Da        Isoelectric Point: 6.3004

>NTDB_id=593613 K1Y48_RS07110 WP_215849997.1 1417240..1418949(+) (pilB) [Acidithiobacillus ferriphilus strain GT2]
MATNPAHITLTPVLRALVSNGLSDEAQLQSLAADPARGKMPLLFYAVERGAVPASVLMAHLSARYNMPMLDLDAVMINEL
LIRKLDKGLMTRYLVLPLSKHGDTLYLAMADPTDFKAVEDVKFNTGLQVLPILVEANKLVKAVNAAANSMQGGIDDVFMD
KPRGEEEQEEFDLAQESDGSVEDAPVVRFARQLLLDAIQRGVSDIHLEPYEKDFRVRYRLDGVLQDVLHPPVALRDGVTS
RLKILCRLDISERRLPQDGRLRVRVPPARVIDFRVSFLPTSFGETIVLRLLDPASSKVPIEQLGFLPDQRKAFEEAIHRP
YGMILVTGPTGSGKTTTLYTALNILNTGDCNISTAEDPVEIPVYGINQVNINERIGLNFATALRSFLRQDPDIIMVGEVR
DLETAETAIKAAQTGHLVLATLHTNDAPQSLTRLENMGIPTYNIAGGVHLVMAQRLARKLCPHCKKPLRIPEQALIEAGF
THEDLQGWRPLGAVGCDQCNNTGYKGRMGLYQVMPVSDAMREIIMRGGTSIDLARQATQEGVLTMRQNGLRRVREGVTSL
EEVLRVTNL

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=593613 K1Y48_RS07110 WP_215849997.1 1417240..1418949(+) (pilB) [Acidithiobacillus ferriphilus strain GT2]
ATGGCTACAAATCCAGCACACATCACGTTGACGCCCGTATTGCGGGCGTTGGTCAGCAACGGGTTAAGCGATGAGGCGCA
GTTGCAAAGCCTTGCCGCCGATCCGGCGCGTGGCAAGATGCCGCTGCTGTTTTATGCGGTTGAGAGGGGTGCCGTTCCTG
CCTCGGTGCTTATGGCGCACCTTTCTGCGCGCTATAACATGCCCATGCTTGATCTGGATGCGGTCATGATTAATGAGTTG
CTTATTCGGAAGCTCGACAAAGGACTGATGACCCGCTATCTGGTGCTGCCCTTGTCGAAGCATGGGGACACCCTCTACCT
CGCCATGGCGGACCCTACGGATTTTAAGGCGGTGGAGGATGTGAAGTTCAACACGGGTCTGCAGGTGTTGCCAATACTGG
TCGAGGCCAATAAGCTGGTAAAGGCGGTGAATGCCGCAGCCAACAGTATGCAAGGCGGGATTGACGATGTTTTCATGGAT
AAACCGCGCGGCGAAGAAGAGCAGGAAGAATTCGATCTTGCCCAGGAGAGTGATGGCAGTGTCGAGGATGCACCCGTGGT
GCGTTTTGCCCGGCAATTGCTGCTGGATGCCATTCAACGCGGGGTTTCCGACATTCATCTGGAACCGTACGAAAAGGACT
TTCGGGTGCGTTACCGACTCGATGGCGTATTGCAGGATGTGTTGCATCCGCCGGTGGCGTTGCGTGACGGTGTCACTTCG
CGCCTGAAGATTCTCTGTCGTCTGGATATTTCTGAGCGGCGTTTACCCCAGGATGGCCGTTTGCGGGTCAGGGTGCCGCC
GGCGCGGGTTATTGATTTCCGGGTTTCTTTTTTGCCCACCAGTTTTGGCGAAACCATTGTGCTGCGCTTGCTGGATCCGG
CCAGTTCCAAGGTGCCCATAGAACAACTGGGCTTTTTGCCGGACCAGCGCAAAGCTTTTGAAGAGGCCATCCACCGGCCC
TACGGTATGATCCTGGTCACCGGCCCCACGGGTTCGGGTAAAACGACGACTCTTTACACCGCGCTGAATATTCTCAATAC
CGGCGACTGCAACATCAGTACCGCAGAGGATCCTGTGGAAATCCCGGTGTACGGGATTAATCAGGTGAATATCAACGAGC
GTATCGGTCTCAACTTTGCCACCGCCTTGCGTTCCTTTCTGCGTCAGGACCCCGACATCATTATGGTCGGTGAGGTGCGT
GATCTGGAAACCGCGGAAACGGCCATCAAAGCCGCCCAGACGGGTCATCTGGTGTTGGCCACCCTGCATACCAATGATGC
ACCACAGAGTCTCACCCGTCTGGAAAATATGGGCATCCCTACCTATAACATAGCGGGCGGTGTGCACTTGGTGATGGCGC
AGCGACTCGCACGCAAACTCTGTCCGCATTGTAAAAAGCCGCTGCGCATTCCCGAACAGGCCCTGATTGAGGCGGGATTT
ACGCACGAGGATCTGCAGGGTTGGCGTCCTCTGGGCGCGGTGGGCTGTGATCAGTGCAATAACACCGGATATAAAGGCCG
GATGGGACTCTATCAGGTGATGCCGGTGAGCGATGCTATGCGTGAAATCATTATGCGCGGTGGTACCTCCATCGATCTGG
CGCGGCAGGCGACACAAGAGGGAGTGCTGACCATGCGGCAAAACGGATTGAGGCGGGTCAGGGAGGGTGTCACTAGTCTT
GAAGAGGTCCTGCGGGTGACCAATCTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-146)

Type II/IV secretion system protein (T2SSE)

(193-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

48.354

100

0.49
  pilB Acinetobacter baumannii D1279779

48.063

99.824

0.48
  pilB Acinetobacter baylyi ADP1

46.479

99.824

0.464
  pilF Neisseria gonorrhoeae MS11

45.276

98.594

0.446
  pilB Vibrio parahaemolyticus RIMD 2210633

43.281

100

0.436
  pilB Vibrio cholerae strain A1552

46.641

91.564

0.427
  pilB Vibrio campbellii strain DS40M4

44.828

91.74

0.411
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.736

93.322

0.371