Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   M2J00_RS24125 Genome accession   NZ_CP096961
Coordinates   5208307..5208771 (-) Length   154 a.a.
NCBI ID   WP_270036282.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NY13932     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5203307..5213771
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2J00_RS24110 (M2J00_24050) nadC 5205817..5206665 (+) 849 WP_003122081.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M2J00_RS24120 (M2J00_24060) - 5206846..5208231 (-) 1386 WP_003122080.1 O-antigen ligase family protein -
  M2J00_RS24125 (M2J00_24065) pilA/pilAI 5208307..5208771 (-) 465 WP_270036282.1 pilin Machinery gene
  M2J00_RS24130 (M2J00_24070) pilB 5209002..5210702 (+) 1701 WP_003122078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M2J00_RS24135 (M2J00_24075) pilC 5210706..5211923 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  M2J00_RS24140 (M2J00_24080) pilD 5211924..5212796 (+) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  M2J00_RS24145 (M2J00_24085) coaE 5212793..5213404 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  M2J00_RS24150 (M2J00_24090) yacG 5213401..5213601 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16307.70 Da        Isoelectric Point: 8.9987

>NTDB_id=593029 M2J00_RS24125 WP_270036282.1 5208307..5208771(-) (pilA/pilAI) [Pseudomonas aeruginosa strain NY13932]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSTTPKDTQYDIGFTE
STLLDGSGKSQIQVTDNKDGTVELVATLGKSSGSAIKGAVITVSRKNDGVWNCKITKTPTAWKPNYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=593029 M2J00_RS24125 WP_270036282.1 5208307..5208771(-) (pilA/pilAI) [Pseudomonas aeruginosa strain NY13932]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTTGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAGGAGATTGTTTCCAGCACGACTCCTAAAGATACCCAGTATGACATTGGCTTCACCGAG
TCTACTTTGCTAGATGGTTCTGGTAAGAGTCAGATCCAGGTAACGGACAATAAAGATGGCACCGTTGAGTTGGTCGCTAC
CTTGGGTAAATCTTCTGGTTCCGCCATCAAAGGGGCTGTAATCACTGTTTCGCGTAAAAATGACGGAGTCTGGAACTGCA
AAATCACCAAAACTCCTACAGCTTGGAAGCCCAACTACGCTCCGGCTAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.444

99.351

0.442
  pilA Acinetobacter baumannii strain A118

44.079

98.701

0.435
  pilA Pseudomonas aeruginosa PAK

46.715

88.961

0.416
  pilA2 Legionella pneumophila strain ERS1305867

38.411

98.052

0.377
  pilA Vibrio parahaemolyticus RIMD 2210633

37.662

100

0.377