Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   MYF74_RS01865 Genome accession   NZ_CP096837
Coordinates   420968..422206 (+) Length   412 a.a.
NCBI ID   WP_071999662.1    Uniprot ID   -
Organism   Escherichia coli strain PS10-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 415968..427206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MYF74_RS01840 (MYF74_01840) mrcA 416248..418800 (-) 2553 WP_001301905.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  MYF74_RS01845 (MYF74_01845) hofM 418920..419699 (+) 780 WP_001308095.1 DNA utilization protein HofM -
  MYF74_RS01850 (MYF74_01850) hofN 419699..420238 (+) 540 WP_023308404.1 DNA utilization protein HofN -
  MYF74_RS01855 (MYF74_01855) hofO 420222..420662 (+) 441 WP_053274135.1 DNA utilization protein HofO -
  MYF74_RS01860 (MYF74_01860) hofP 420652..421056 (+) 405 WP_028132028.1 DNA utilization protein HofP -
  MYF74_RS01865 (MYF74_01865) comE 420968..422206 (+) 1239 WP_071999662.1 DNA uptake porin HofQ Machinery gene
  MYF74_RS01870 (MYF74_01870) aroK 422607..423128 (+) 522 WP_000818618.1 shikimate kinase AroK -
  MYF74_RS01875 (MYF74_01875) aroB 423185..424273 (+) 1089 WP_000439848.1 3-dehydroquinate synthase -
  MYF74_RS01880 (MYF74_01880) damX 424365..425651 (+) 1287 WP_000343195.1 cell division protein DamX -
  MYF74_RS01885 (MYF74_01885) dam 425758..426594 (+) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44765.26 Da        Isoelectric Point: 5.9488

>NTDB_id=590927 MYF74_RS01865 WP_071999662.1 420968..422206(+) (comE) [Escherichia coli strain PS10-3]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNDNIARQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSTKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=590927 MYF74_RS01865 WP_071999662.1 420968..422206(+) (comE) [Escherichia coli strain PS10-3]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGTCAGGAGGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATGACAATATCGCCCGTCAGGAAGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCTGGGGAGAAGCTACTGAGTACCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCCCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGTTTGCTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAAGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCAGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTGCCGTTGCTTGGTGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  comE Haemophilus influenzae 86-028NP

36.927

100

0.391
  pilQ Vibrio campbellii strain DS40M4

38.242

100

0.391
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364
  pilQ Pseudomonas aeruginosa PAK

34.174

100

0.362