Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   MYF75_RS17110 Genome accession   NZ_CP096834
Coordinates   3212768..3213892 (+) Length   374 a.a.
NCBI ID   WP_000228531.1    Uniprot ID   A7ZSH4
Organism   Escherichia coli strain PM4-5     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3207768..3218892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MYF75_RS17085 (MYF75_17085) mscL 3207894..3208304 (-) 411 WP_000022442.1 large-conductance mechanosensitive channel protein MscL -
  MYF75_RS17090 (MYF75_17090) trkA 3208434..3209810 (-) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  MYF75_RS17095 (MYF75_17095) rsmB 3209832..3211121 (-) 1290 WP_000744779.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  MYF75_RS17100 (MYF75_17100) fmt 3211167..3212114 (-) 948 WP_000004432.1 methionyl-tRNA formyltransferase -
  MYF75_RS17105 (MYF75_17105) def 3212129..3212638 (-) 510 WP_000114984.1 peptide deformylase -
  MYF75_RS17110 (MYF75_17110) dprA 3212768..3213892 (+) 1125 WP_000228531.1 DNA-protecting protein DprA Machinery gene
  MYF75_RS17115 (MYF75_17115) smg 3213864..3214337 (+) 474 WP_000460672.1 DUF494 family protein Smg -
  MYF75_RS17120 (MYF75_17120) yrdD 3214366..3214908 (+) 543 WP_001129723.1 type I DNA topoisomerase -
  MYF75_RS17125 (MYF75_17125) tsaC 3214913..3215485 (+) 573 WP_001297709.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  MYF75_RS17130 (MYF75_17130) aroE 3215490..3216308 (+) 819 WP_000451211.1 shikimate dehydrogenase -
  MYF75_RS17135 (MYF75_17135) yrdB 3216305..3216562 (+) 258 WP_001070563.1 DUF1488 domain-containing protein -
  MYF75_RS17140 (MYF75_17140) yrdA 3216538..3217092 (-) 555 WP_001286216.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40986.98 Da        Isoelectric Point: 6.3122

>NTDB_id=590893 MYF75_RS17110 WP_000228531.1 3212768..3213892(+) (dprA) [Escherichia coli strain PM4-5]
MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLMTTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=590893 MYF75_RS17110 WP_000228531.1 3212768..3213892(+) (dprA) [Escherichia coli strain PM4-5]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCAAATTGATGCGGTTGGATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGATGACGACAGATTACCCCGGTGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGGTTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCTGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCCCGACTGGCTGCCAGTCTGCT
TGAACATGGCGGAGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGCAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (WHD_DprA)

(312-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZSH4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.509

98.663

0.479
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

44.865

98.93

0.444
  dprA Vibrio cholerae strain A1552

44.865

98.93

0.444
  dprA Haemophilus influenzae Rd KW20

45.401

90.107

0.409
  dprA Glaesserella parasuis strain SC1401

44.838

90.642

0.406
  dprA Legionella pneumophila strain ERS1305867

44.545

88.235

0.393