Detailed information NTG0590296

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KZA74_RS14200
• Genome accession: NZ_CP079931
• Coordinates: 2979437..2980555 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain 17978UN
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2974437..2985555

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KZA74_RS14190 (KZA74_14200)	-	2977553..2978245 (-)	693	WP_001108509.1	YggS family pyridoxal phosphate-dependent enzyme	-
KZA74_RS14195 (KZA74_14205)	pilT	2978372..2979409 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
KZA74_RS14200 (KZA74_14210)	pilU	2979437..2980555 (+)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KZA74_RS14205 (KZA74_14215)	fur	2980625..2981062 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
KZA74_RS14210 (KZA74_14220)	-	2981174..2981572 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
KZA74_RS14215 (KZA74_14225)	-	2981627..2981950 (-)	324	WP_000442590.1	RnfH family protein	-
KZA74_RS14220 (KZA74_14230)	-	2981950..2983017 (-)	1068	WP_004748097.1	hypothetical protein	-
KZA74_RS14225 (KZA74_14235)	-	2983170..2983625 (-)	456	WP_000782976.1	bacteriohemerythrin	-
KZA74_RS14230 (KZA74_14240)	-	2983878..2984726 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=590296 KZA74_RS14200 WP_000347034.1 2979437..2980555(+) (pilU) [Acinetobacter baumannii strain 17978UN]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=590296 KZA74_RS14200 WP_000347034.1 2979437..2980555(+) (pilU) [Acinetobacter baumannii strain 17978UN]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TTATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39