Detailed information NTG0588840

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KXJ79_RS14050
• Genome accession: NZ_CP079716
• Coordinates: 3265139..3266308 (-)
• Length: 389 a.a.
• NCBI ID: WP_054071721.1
• Uniprot ID: A0A9P3AB78
• Organism: Pseudomonas amygdali strain HS1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3260139..3271308

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KXJ79_RS14035 (KXJ79_14035)	-	3261423..3262487 (+)	1065	WP_058408116.1	microcin C ABC transporter permease YejB	-
KXJ79_RS14040 (KXJ79_14040)	-	3262487..3263509 (+)	1023	WP_054097554.1	ABC transporter permease	-
KXJ79_RS14045 (KXJ79_14045)	-	3263511..3265088 (+)	1578	WP_011168500.1	ABC transporter ATP-binding protein	-
KXJ79_RS14050 (KXJ79_14050)	pilU	3265139..3266308 (-)	1170	WP_054071721.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KXJ79_RS14055 (KXJ79_14055)	-	3266432..3266707 (+)	276	WP_002553334.1	peptidylprolyl isomerase	-
KXJ79_RS14060 (KXJ79_14060)	pgm	3266782..3268428 (-)	1647	WP_219169147.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
KXJ79_RS14065 (KXJ79_14065)	-	3268544..3270871 (-)	2328	WP_219169149.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42578.79 Da
• Isoelectric Point: 6.0641

>NTDB_id=588840 KXJ79_RS14050 WP_054071721.1 3265139..3266308(-) (pilU) [Pseudomonas amygdali strain HS1]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVIAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGVASLTTPPPAPIGSSTASTAEWGLVDDDAPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=588840 KXJ79_RS14050 WP_054071721.1 3265139..3266308(-) (pilU) [Pseudomonas amygdali strain HS1]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTATCTTTCCACCGGAGCGCCTCCGTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTGGGCAGTGAAACCCTCAAGCCGGGCGAGGTGGCGGTGATTGCCCAGGGGC
TGATGGACGAGGAGCAGAAGCTCGAATTCCTGCGCGAACTGGAGATGAACCTTGCTGTTTCGCTGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGTAACGAGGTGTCCATCGTCGCGCGGAATATCAAGCTGGATATTCCGCGTTTCGA
GGACCTGTTCCTGCCGCCCGTACTGCTCGATGTGATCATGGAAAAGCACGGGCTGGTGTTGTTCGTCGGTGCCACCGGTT
CGGGCAAATCGACCTCGCTGGCGGCGCTGATCGATTACCGCAATCGCAACGCCAGCGGCCATATCATCACCATTGAAGAC
CCGGTCGAATTTATCCATCGGCACAAGAAGTCCATCGTCAACCAGCGCGAAGTGGGGGTTGATACCCGCAGTTTCCGCGC
GGCGCTGAAGAACACGTTGCGTCAGGCTCCGGATGTGATTCTGATCGGCGAAATTCGTGATCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGCCACTTGGCGATCTCGACCCTGCATGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAGCGTCGTGCGCAGTTGCTGCATGACCTGGGCAATAACCTCAAGGCGTTCGTGTCCCAGCG
GCTGGTCAGAACCCCGGACGGCAAACGTCGCGCAGCGGTGGAGGTGATGATGGGCACGCCGACCATTCGCGACCTGATTC
AGCGCAATGAACTGACCGAGCTCAAGGGCATCATGGAGAAATCCGGCAGCCTCGGTATGCAGACCTTCGACACGGCGTTG
TTCAATCTGGCCGTCGAGGGGGCGATCAGCGAAGAGGAAGCGCTGAAGAATGCCGACTCGCAGAACAACGTGCGTCTGCG
CCTCAAACTGCACAGTGAAGGCGGAGTCGCCAGCCTGACGACGCCGCCCCCGGCCCCGATCGGCAGCAGCACGGCAAGTA
CCGCCGAGTGGGGGCTGGTCGATGACGACGCGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.011	93.059	0.54
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilU	Vibrio cholerae strain A1552	51.714	89.974	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.687	98.458	0.391
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362