Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KV708_RS03870 Genome accession   NZ_CP079703
Coordinates   854186..855928 (-) Length   580 a.a.
NCBI ID   WP_046461087.1    Uniprot ID   A0A5S4SZP2
Organism   Comamonas thiooxydans strain N1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 849186..860928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KV708_RS03840 (KV708_03835) - 849701..850171 (+) 471 WP_046461083.1 NUDIX domain-containing protein -
  KV708_RS03845 (KV708_03840) - 850289..850498 (-) 210 WP_034357520.1 DNA gyrase inhibitor YacG -
  KV708_RS03850 (KV708_03845) zapD 850602..851357 (-) 756 WP_046461085.1 cell division protein ZapD -
  KV708_RS03855 (KV708_03850) coaE 851445..852071 (-) 627 WP_057091557.1 dephospho-CoA kinase -
  KV708_RS03860 (KV708_03855) - 852075..852944 (-) 870 WP_003062192.1 A24 family peptidase -
  KV708_RS03865 (KV708_03860) pilC 852944..854161 (-) 1218 WP_003062193.1 type II secretion system F family protein Machinery gene
  KV708_RS03870 (KV708_03865) pilB 854186..855928 (-) 1743 WP_046461087.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KV708_RS03880 (KV708_03875) - 856182..857111 (-) 930 WP_003062198.1 polyprenyl synthetase family protein -
  KV708_RS03885 (KV708_03880) rplU 857454..857765 (+) 312 WP_003058536.1 50S ribosomal protein L21 -
  KV708_RS03890 (KV708_03885) rpmA 857782..858039 (+) 258 WP_003058534.1 50S ribosomal protein L27 -
  KV708_RS03895 (KV708_03890) cgtA 858196..859311 (+) 1116 WP_057091558.1 Obg family GTPase CgtA -
  KV708_RS03900 (KV708_03895) proB 859422..860561 (+) 1140 WP_003062203.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 63116.36 Da        Isoelectric Point: 5.1537

>NTDB_id=588723 KV708_RS03870 WP_046461087.1 854186..855928(-) (pilB) [Comamonas thiooxydans strain N1]
MAAADTLQKNTPAPIAIPGLGRALISAGKVSQQAAENAAKKAATGKTPFITELSQSGEISALEIAETISMMFSTPLLDLN
AIDVQQLPRELLDPKISNAYRVLALSKRGNRLTIATADPTQQEAAEQIKFTTQLLVDWVVVEADKLQKLIEQTGKSVSES
LDSYSSSGDFDFDDVKIEDAPEEKDNAVGAEVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHNYRVRFRIDGELREIAS
PPIAIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEE
KERLLKAINRPYGMILVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINMPGVNQVNVNEKAGLTFAAALKSFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCAQCKEPAD
IPHDALIDAGYDEAELDGSWVSYKPVGCPACNNGYKGRVGIYQVMPISEEIQRIILRDGSALEIAAQAKAEGVRSLRGSG
LHKVKLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=588723 KV708_RS03870 WP_046461087.1 854186..855928(-) (pilB) [Comamonas thiooxydans strain N1]
ATGGCTGCTGCCGACACTCTGCAAAAGAACACTCCTGCGCCCATTGCCATCCCAGGTTTAGGCAGGGCCCTGATTTCTGC
AGGAAAGGTTTCGCAGCAAGCCGCCGAGAACGCAGCCAAGAAAGCCGCCACAGGCAAGACCCCGTTCATCACCGAGCTCA
GCCAGTCTGGTGAAATCAGCGCACTGGAAATTGCCGAAACCATTTCCATGATGTTCAGCACTCCGCTGCTGGACCTCAAC
GCCATCGACGTCCAGCAACTTCCGCGCGAGCTGCTAGACCCGAAAATCAGCAACGCCTATCGCGTGCTGGCACTCAGCAA
GCGCGGCAATCGGCTCACCATCGCCACGGCAGACCCTACTCAACAGGAAGCTGCCGAACAGATCAAATTCACGACTCAGT
TGCTGGTGGACTGGGTGGTTGTCGAGGCCGACAAGCTGCAAAAGCTCATCGAGCAGACCGGCAAGAGCGTCAGCGAATCC
CTGGACTCCTATTCCAGCTCAGGGGACTTCGACTTTGACGACGTCAAGATCGAGGATGCCCCCGAGGAAAAAGACAATGC
CGTCGGCGCCGAGGTCGAGGATGCGCCGGTCGTCAAGTTTCTGCACAAGATGCTGCTGGACGCCTTCAATATGCGCGCGT
CCGACTTGCACTTCGAGCCCTACGAACACAATTACCGCGTGCGCTTTCGTATCGACGGCGAGCTGCGCGAGATTGCCTCT
CCTCCTATTGCGATCAAGGACAAGCTGGCTTCGCGCATCAAGGTGATCTCGCGCCTGGACATCTCGGAGAAACGCGTTCC
GCAAGACGGCCGCATGAAGCTCAAGGTCGGCCCCGATCGCGTCATCGACTTTCGTGTCAGCACCTTGCCCACGCTGTTTG
GCGAAAAGATCGTGATCCGTATTCTGGACCCGAGCTCGGCCAAGATGGGCATCGATGCCCTAGGCTACGAGCCCGAGGAG
AAAGAGCGCCTGCTCAAGGCCATCAACCGCCCCTACGGCATGATTCTGGTCACCGGCCCCACGGGCTCGGGCAAGACCGT
GTCGCTGTACACCTGCCTGAACCTGCTCAACCAGCCGGGCGTGAACATTGCCACTGCCGAAGACCCTTCCGAAATCAACA
TGCCCGGCGTCAACCAGGTGAATGTGAACGAGAAGGCCGGCCTGACCTTTGCTGCTGCGCTCAAGTCCTTTCTGCGCCAG
GATCCGGACATCATCATGGTCGGTGAAATCCGCGACCTGGAAACCGCCGACATCTCGATCAAGGCTGCCCAGACCGGGCA
CCTGGTGCTGTCCACGCTGCACACCAATGACGCTCCGACCACGCTGACGCGCATGCGCAATATGGGCATCGCCCCTTTCA
ATATTGCCTCCAGCGTGATCCTGATCACCGCCCAGCGTCTGGCGCGACGCCTGTGTGCGCAGTGCAAGGAGCCCGCCGAC
ATACCGCACGACGCCCTGATCGATGCCGGCTACGACGAAGCCGAACTCGACGGCAGCTGGGTCAGCTACAAGCCTGTCGG
CTGCCCTGCCTGCAACAACGGCTACAAGGGACGTGTCGGTATTTATCAGGTGATGCCCATCAGCGAAGAGATTCAGCGCA
TCATCCTGCGTGACGGCAGCGCCCTGGAGATTGCCGCACAGGCCAAGGCCGAAGGAGTACGCTCTCTGCGCGGCTCGGGC
CTGCATAAAGTCAAGCTGGGTCTGACCTCACTCGAAGAAGTGCTGGCGGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4SZP2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.53

98.966

0.54
  pilB Acinetobacter baumannii D1279779

55.203

97.759

0.54
  pilF Neisseria gonorrhoeae MS11

52.473

97.586

0.512
  pilB Legionella pneumophila strain ERS1305867

51.237

97.586

0.5
  pilB Vibrio cholerae strain A1552

47.08

94.483

0.445
  pilB Vibrio campbellii strain DS40M4

44.207

96.724

0.428
  pilB Vibrio parahaemolyticus RIMD 2210633

46.198

90.69

0.419
  pilF Thermus thermophilus HB27

37.925

100

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.421

98.276

0.378