Detailed information NTG0587678

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: KW114_RS11835
• Genome accession: NZ_CP079097
• Coordinates: 2547480..2548541 (+)
• Length: 353 a.a.
• NCBI ID: WP_017365267.1
• Uniprot ID: -
• Organism: Methylococcus capsulatus strain KN2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2542480..2553541

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KW114_RS11815 (KW114_11815)	-	2542968..2543741 (-)	774	WP_010959682.1	exodeoxyribonuclease III	-
KW114_RS11820 (KW114_11820)	rpmE	2543856..2544059 (+)	204	WP_010959683.1	50S ribosomal protein L31	-
KW114_RS11825 (KW114_11825)	-	2544095..2544706 (-)	612	WP_026045942.1	hypothetical protein	-
KW114_RS11830 (KW114_11830)	-	2544722..2547169 (-)	2448	WP_256996377.1	penicillin-binding protein 1A	-
KW114_RS11835 (KW114_11835)	pilM	2547480..2548541 (+)	1062	WP_017365267.1	pilus assembly protein PilM	Machinery gene
KW114_RS11840 (KW114_11840)	-	2548541..2549146 (+)	606	WP_017365266.1	PilN domain-containing protein	-
KW114_RS11845 (KW114_11845)	-	2549143..2549781 (+)	639	WP_017365265.1	type 4a pilus biogenesis protein PilO	-
KW114_RS11850 (KW114_11850)	-	2549781..2550323 (+)	543	WP_010959689.1	pilus assembly protein PilP	-
KW114_RS11855 (KW114_11855)	pilQ	2550439..2552499 (+)	2061	WP_238536855.1	type IV pilus secretin PilQ	-
KW114_RS11860 (KW114_11860)	aroK	2552561..2553100 (+)	540	WP_010959691.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 353 a.a.
• Molecular weight: 38425.92 Da
• Isoelectric Point: 4.9350

>NTDB_id=587678 KW114_RS11835 WP_017365267.1 2547480..2548541(+) (pilM) [Methylococcus capsulatus strain KN2]
MWGLGRKKPLLLGIDISAAAVKLLELSRKNGGYQVESYGVVPLPRNTVVDNTLAEPDNVSAAVRAVVKQSGTALRHAVVA
VPGSVVITKRIALPANLEGDELEAQIELEADQYIPHAREEVSLDFEVLGKNPKNPDLLDVLLVATRRENVEDRVSVLENA
GLGVEIVDVESYAIERAFELVRDQLPPLLRDRAVAVADVGAMATTLNVIHGGSIIYTREQGFGGMQLTDEIQRRYGLTYE
EAGLAKKEGSLPGNYAEEVLEPFKHALVQQISRSFQFYLSSTAQRGFDAVVLAGGCAMIPGIDRYVEAALQVPTVIANPF
RHMSFSGRVRQERLSYDSSALMLACGLALRSFD

Nucleotide

• Length: 1062 bp

>NTDB_id=587678 KW114_RS11835 WP_017365267.1 2547480..2548541(+) (pilM) [Methylococcus capsulatus strain KN2]
ATGTGGGGCTTAGGAAGGAAGAAACCGCTCCTGCTGGGGATCGACATCAGCGCGGCGGCGGTCAAGCTGCTCGAGCTGAG
CAGAAAGAACGGCGGCTACCAGGTGGAAAGCTATGGCGTTGTGCCTTTGCCCCGCAACACGGTGGTCGACAACACCCTTG
CCGAGCCGGACAACGTGTCGGCGGCGGTCCGGGCCGTGGTCAAACAGTCGGGGACCGCGCTCAGGCACGCGGTGGTCGCT
GTGCCTGGCTCGGTGGTCATCACCAAGCGCATCGCCCTACCCGCCAACCTGGAGGGCGACGAACTCGAGGCGCAGATCGA
ACTGGAGGCGGACCAGTACATTCCTCACGCCAGGGAGGAAGTCAGCCTGGATTTCGAGGTGCTGGGGAAGAATCCGAAGA
ATCCCGATCTGCTGGATGTGCTGCTGGTGGCGACTCGCCGGGAGAACGTGGAAGACCGGGTCTCGGTACTGGAAAATGCC
GGACTCGGCGTCGAGATCGTCGATGTCGAGTCCTATGCCATCGAACGCGCCTTCGAACTTGTCCGGGATCAGCTTCCACC
GCTCCTCCGCGACCGGGCGGTTGCCGTAGCCGATGTGGGCGCCATGGCCACGACGCTCAACGTCATCCATGGCGGTTCCA
TCATCTACACGCGGGAACAGGGTTTCGGCGGCATGCAGCTCACCGACGAGATCCAGCGGCGCTACGGTTTGACCTACGAG
GAAGCGGGGCTGGCGAAGAAGGAGGGGAGCCTTCCCGGCAACTATGCCGAAGAGGTGCTGGAGCCGTTCAAGCATGCCTT
GGTTCAGCAGATCAGCCGATCGTTCCAGTTCTATCTGTCGTCCACTGCGCAGCGTGGTTTCGATGCGGTGGTGCTGGCCG
GCGGCTGCGCCATGATTCCGGGGATAGACCGTTACGTCGAGGCGGCGTTGCAGGTTCCCACCGTGATTGCCAATCCCTTC
CGTCACATGAGTTTTTCGGGAAGGGTGCGGCAGGAGCGCCTGAGTTACGATTCATCCGCCCTGATGCTGGCATGCGGCCT
GGCACTGCGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-352)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	51.603	97.167	0.501
comM	Acinetobacter nosocomialis M2	51.02	97.167	0.496
comM	Acinetobacter baylyi ADP1	49.138	98.584	0.484
pilM	Legionella pneumophila strain ERS1305867	42.733	97.45	0.416