Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   MW396_RS02250 Genome accession   NZ_CP095538
Coordinates   446262..447755 (+) Length   497 a.a.
NCBI ID   WP_000249314.1    Uniprot ID   -
Organism   Escherichia coli strain GN04592     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 441262..452755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW396_RS02230 (MW396_02230) pilD 441910..442719 (+) 810 WP_000895878.1 prepilin peptidase PppA Machinery gene
  MW396_RS02235 (MW396_02235) gspS2 442785..443195 (+) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  MW396_RS02240 (MW396_02240) gspC 443213..444172 (+) 960 WP_000135091.1 type II secretion system protein GspC -
  MW396_RS02245 (MW396_02245) gspD 444202..446262 (+) 2061 WP_000498830.1 type II secretion system secretin GspD -
  MW396_RS02250 (MW396_02250) pilB/pilB1 446262..447755 (+) 1494 WP_000249314.1 type II secretion system ATPase GspE Machinery gene
  MW396_RS02255 (MW396_02255) gspF 447755..448978 (+) 1224 WP_001173444.1 type II secretion system inner membrane protein GspF -
  MW396_RS02260 (MW396_02260) gspG 448995..449450 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  MW396_RS02265 (MW396_02265) gspH 449454..450017 (+) 564 WP_001115129.1 type II secretion system minor pseudopilin GspH -
  MW396_RS02270 (MW396_02270) gspI 450014..450385 (+) 372 WP_000820072.1 type II secretion system minor pseudopilin GspI -
  MW396_RS02275 (MW396_02275) gspJ 450382..450987 (+) 606 WP_001250454.1 type II secretion system minor pseudopilin GspJ -
  MW396_RS02280 (MW396_02280) gspK 450984..451961 (+) 978 WP_000633198.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55170.30 Da        Isoelectric Point: 6.0089

>NTDB_id=583894 MW396_RS02250 WP_000249314.1 446262..447755(+) (pilB/pilB1) [Escherichia coli strain GN04592]
MVPVAQETSANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLAGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=583894 MW396_RS02250 WP_000249314.1 446262..447755(+) (pilB/pilB1) [Escherichia coli strain GN04592]
ATGGTGCCTGTAGCACAGGAAACCTCCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGCCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAACGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAAGCTCGCCAGTTGATGGAAGATCTCGGTTCCGCCGAGGACTTCTTTACCCTCGCAGAAGAGCTGCCAGAAACGGAAGA
TTTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATTAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTAGACGGAACGCTACATGAAATGCTGCGT
CCCGGGCGCAAACTAGCCTCGCTGCTGGTGTCGCGTATCAAGGTGATGGCGCGGCTGGACATTGCCGAAAAGCGCGTGCC
GCAGGATGGACGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTGAGTCATGAACTGACC
GCGCAGTTGCGCCAGCTACTGCACAAACCGCACGGTATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATTGCCGTCCAGGCATCGCTTACAGGGCACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTTGAGCCTTTCCTGC
TCTCTTCCAGTCTGGCGGGCGTGATGGCGCAGCGGCTGGTTCGCACGCTGTGCCCCGACTGCCGCCAGCCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGAACCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAACTGATTGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGTAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.765

85.513

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Legionella pneumophila strain ERS1305867

43.387

86.72

0.376
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilF Thermus thermophilus HB27

45

80.483

0.362