Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   KU529_RS01635 Genome accession   NZ_CP077856
Coordinates   325853..326350 (+) Length   165 a.a.
NCBI ID   WP_078098371.1    Uniprot ID   -
Organism   Staphylococcus aureus strain L5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 320853..331350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU529_RS01595 (KU529_01600) - 320897..321883 (+) 987 WP_000161311.1 ROK family glucokinase -
  KU529_RS01600 (KU529_01605) - 321883..322212 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  KU529_RS01605 (KU529_01610) - 322209..322832 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  KU529_RS01610 (KU529_01615) comGA 322884..323858 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  KU529_RS01615 (KU529_01620) comGB 323830..324900 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  KU529_RS01620 (KU529_01625) comGC 324914..325225 (+) 312 WP_031763468.1 competence type IV pilus major pilin ComGC Machinery gene
  KU529_RS01625 (KU529_01630) comGD 325203..325649 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  KU529_RS01630 (KU529_01635) comGE 325636..325935 (+) 300 WP_000844410.1 hypothetical protein Machinery gene
  KU529_RS01635 (KU529_01640) comGF 325853..326350 (+) 498 WP_078098371.1 competence type IV pilus minor pilin ComGF Machinery gene
  KU529_RS01640 (KU529_01645) - 326447..326593 (+) 147 WP_001792109.1 hypothetical protein -
  KU529_RS01645 (KU529_01650) - 326583..327107 (+) 525 WP_001015120.1 shikimate kinase -
  KU529_RS01650 (KU529_01655) gcvT 327266..328357 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  KU529_RS01655 (KU529_01660) gcvPA 328377..329723 (+) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  KU529_RS01660 (KU529_01665) gcvPB 329716..331188 (+) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19155.01 Da        Isoelectric Point: 10.4428

>NTDB_id=583893 KU529_RS01635 WP_078098371.1 325853..326350(+) (comGF) [Staphylococcus aureus strain L5]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEKMIEHKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=583893 KU529_RS01635 WP_078098371.1 325853..326350(+) (comGF) [Staphylococcus aureus strain L5]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAAAAAT
GATCGAACACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.788

100

0.988
  comGF Staphylococcus aureus MW2

95.152

100

0.952