Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   MW395_RS22940 Genome accession   NZ_CP095526
Coordinates   4569448..4570686 (+) Length   412 a.a.
NCBI ID   WP_000815963.1    Uniprot ID   -
Organism   Escherichia coli strain GN05505     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4564448..4575686
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW395_RS22915 (MW395_22910) mrcA 4564728..4567280 (-) 2553 WP_001301905.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  MW395_RS22920 (MW395_22915) hofM 4567400..4568179 (+) 780 WP_001377090.1 DNA utilization protein HofM -
  MW395_RS22925 (MW395_22920) hofN 4568179..4568718 (+) 540 WP_001069323.1 DNA utilization protein HofN -
  MW395_RS22930 (MW395_22925) hofO 4568702..4569142 (+) 441 WP_000367364.1 DNA utilization protein HofO -
  MW395_RS22935 (MW395_22930) hofP 4569132..4569536 (+) 405 WP_001322941.1 DNA utilization protein HofP -
  MW395_RS22940 (MW395_22935) comE 4569448..4570686 (+) 1239 WP_000815963.1 DNA uptake porin HofQ Machinery gene
  MW395_RS22945 (MW395_22940) aroK 4571087..4571608 (+) 522 WP_000818618.1 shikimate kinase AroK -
  MW395_RS22950 (MW395_22945) aroB 4571665..4572753 (+) 1089 WP_000439850.1 3-dehydroquinate synthase -
  MW395_RS22955 (MW395_22950) damX 4572845..4574131 (+) 1287 WP_000343222.1 cell division protein DamX -
  MW395_RS22960 (MW395_22955) dam 4574238..4575074 (+) 837 WP_000742137.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44760.29 Da        Isoelectric Point: 6.1846

>NTDB_id=583705 MW395_RS22940 WP_000815963.1 4569448..4570686(+) (comE) [Escherichia coli strain GN05505]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVRGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KAALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=583705 MW395_RS22940 WP_000815963.1 4569448..4570686(+) (comE) [Escherichia coli strain GN05505]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACACTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG
TCAGAGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAAGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGGCAGGAAGGCAATATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAGCGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGTTGGCTGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTTACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGTGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae 86-028NP

37.3

100

0.396
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  pilQ Vibrio campbellii strain DS40M4

38.242

100

0.391
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  comE Glaesserella parasuis strain SC1401

35.952

100

0.367
  pilQ Pseudomonas aeruginosa PAK

34.174

100

0.362