NTDB

Overview

Name	comE	Type	Machinery gene
Locus tag	MW398_RS12275	Genome accession	NZ_CP095505
Coordinates	2259137..2260375 (-)	Length	412 a.a.
NCBI ID	WP_000816006.1	Uniprot ID	-
Organism	Escherichia coli strain GN02050
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2254137..2265375

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MW398_RS12255 (MW398_12245)	dam	2254749..2255585 (-)	837	WP_000742141.1	adenine-specific DNA-methyltransferase	-
MW398_RS12260 (MW398_12250)	damX	2255692..2256978 (-)	1287	WP_021524563.1	cell division protein DamX	-
MW398_RS12265 (MW398_12255)	aroB	2257070..2258158 (-)	1089	WP_000439850.1	3-dehydroquinate synthase	-
MW398_RS12270 (MW398_12260)	aroK	2258215..2258736 (-)	522	WP_000818618.1	shikimate kinase AroK	-
MW398_RS12275 (MW398_12265)	comE	2259137..2260375 (-)	1239	WP_000816006.1	DNA uptake porin HofQ	Machinery gene
MW398_RS12280 (MW398_12270)	hofP	2260287..2260691 (-)	405	WP_001264137.1	DNA utilization protein HofP	-
MW398_RS12285 (MW398_12275)	hofO	2260681..2261121 (-)	441	WP_021524564.1	DNA utilization protein HofO	-
MW398_RS12290 (MW398_12280)	hofN	2261105..2261644 (-)	540	WP_021524565.1	DNA utilization protein HofN	-
MW398_RS12295 (MW398_12285)	hofM	2261644..2262423 (-)	780	WP_021524566.1	DNA utilization protein HofM	-
MW398_RS12300 (MW398_12290)	mrcA	2262543..2265095 (+)	2553	WP_001298208.1	peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA	-

Sequence

Protein

Download Length: 412 a.a. Molecular weight: 44797.26 Da Isoelectric Point: 5.9488

                                >NTDB_id=583245 MW398_RS12275 WP_000816006.1 2259137..2260375(-) (comE) [Escherichia coli strain GN02050]

MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL

ITRQEGNILSVHSVAWQNDNIARQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN

KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQGGGVGQVTTLGSDLSVATATTHIGFNIGRIN

GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL

HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL

VVFITPRLVSSE

Nucleotide

Download Length: 1239 bp

                                >NTDB_id=583245 MW398_RS12275 WP_000816006.1 2259137..2260375(-) (comE) [Escherichia coli strain GN02050]

ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT

GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTTTCGCCAGACG

TCAGCGGTACGGTGTCGTTACATTTAACTGACGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGATTG

ATAACGCGCCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA

GCAGACGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCTGACGCCGGAGAGCTGGCGA

AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC

AAAACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT

TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGTTGGCCGATGCGCAACAAGGTGGTGGCGTTG

GGCAAGTCACTACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATATCGGTTTTAACATTGGACGCATCAAC

GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGTTGGATATTATCGCCAGTCCGCGTCTGCTGGC

CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT

CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA

CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA

AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA

GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA

GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comE	Haemophilus influenzae 86-028NP	37.3	100	0.396
pilQ	Vibrio campbellii strain DS40M4	38.48	100	0.393
comE	Haemophilus influenzae Rd KW20	37.156	100	0.393
pilQ	Vibrio cholerae strain A1552	37.772	100	0.379
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	37.772	100	0.379
comE	Glaesserella parasuis strain SC1401	35.782	100	0.367
pilQ	Pseudomonas aeruginosa PAK	34.633	100	0.367

Detailed information NTG0583245