Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   MWN28_RS02360 Genome accession   NZ_CP095448
Coordinates   501685..502809 (+) Length   374 a.a.
NCBI ID   WP_000228526.1    Uniprot ID   B7UK09
Organism   Escherichia coli strain LS45     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 496685..507809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWN28_RS02335 (MWN28_02335) mscL 496811..497221 (-) 411 WP_000022442.1 large-conductance mechanosensitive channel protein MscL -
  MWN28_RS02340 (MWN28_02340) trkA 497351..498727 (-) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  MWN28_RS02345 (MWN28_02345) rsmB 498749..500038 (-) 1290 WP_012311980.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  MWN28_RS02350 (MWN28_02350) fmt 500084..501031 (-) 948 WP_000004468.1 methionyl-tRNA formyltransferase -
  MWN28_RS02355 (MWN28_02355) def 501046..501555 (-) 510 WP_000114986.1 peptide deformylase -
  MWN28_RS02360 (MWN28_02360) dprA 501685..502809 (+) 1125 WP_000228526.1 DNA-protecting protein DprA Machinery gene
  MWN28_RS02365 (MWN28_02365) smg 502781..503254 (+) 474 WP_000460672.1 DUF494 family protein Smg -
  MWN28_RS02370 (MWN28_02370) yrdD 503283..503825 (+) 543 WP_001129720.1 type I DNA topoisomerase -
  MWN28_RS02375 (MWN28_02375) tsaC 503830..504402 (+) 573 WP_001297709.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  MWN28_RS02380 (MWN28_02380) aroE 504407..505225 (+) 819 WP_000451244.1 shikimate dehydrogenase -
  MWN28_RS02385 (MWN28_02385) yrdB 505222..505479 (+) 258 WP_001070584.1 DUF1488 domain-containing protein -
  MWN28_RS02390 (MWN28_02390) yrdA 505455..506009 (-) 555 WP_001286206.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40992.90 Da        Isoelectric Point: 6.2662

>NTDB_id=582898 MWN28_RS02360 WP_000228526.1 501685..502809(+) (dprA) [Escherichia coli strain LS45]
MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLSWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=582898 MWN28_RS02360 WP_000228526.1 501685..502809(+) (dprA) [Escherichia coli strain LS45]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACTGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTCTTGGTTGGAGCAACCCAACCATCATTTAATTCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTTGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAACGCTGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTAGGGAATGGACTTAATACCATTCATCCGCGTCGCCATGCCCGACTGGCTACCAGTTTGCT
TGAACATGGTGGGGCTCTTGTCTCGGAATTTCCCCTCGATGTTCCTCCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCCTTGCCGGGTCCATTAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTAACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (WHD_DprA)

(312-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7UK09

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.509

98.663

0.479
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

48.71

82.888

0.404
  dprA Vibrio cholerae strain A1552

48.71

82.888

0.404
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Haemophilus influenzae Rd KW20

43.917

90.107

0.396
  dprA Legionella pneumophila strain ERS1305867

43.363

90.642

0.393