Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   MUK77_RS01695 Genome accession   NZ_CP095086
Coordinates   371601..372839 (+) Length   412 a.a.
NCBI ID   WP_000816009.1    Uniprot ID   -
Organism   Escherichia coli isolate EC5654     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 366601..377839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUK77_RS01670 (MUK77_01670) mrcA 366881..369433 (-) 2553 WP_001298208.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  MUK77_RS01675 (MUK77_01675) hofM 369553..370332 (+) 780 WP_001308095.1 DNA utilization protein HofM -
  MUK77_RS01680 (MUK77_01680) hofN 370332..370871 (+) 540 WP_001069340.1 DNA utilization protein HofN -
  MUK77_RS01685 (MUK77_01685) hofO 370855..371295 (+) 441 WP_001055744.1 DNA utilization protein HofO -
  MUK77_RS01690 (MUK77_01690) hofP 371285..371689 (+) 405 WP_001264138.1 DNA utilization protein HofP -
  MUK77_RS01695 (MUK77_01695) comE 371601..372839 (+) 1239 WP_000816009.1 DNA uptake porin HofQ Machinery gene
  MUK77_RS01700 (MUK77_01700) aroK 373240..373761 (+) 522 WP_000818618.1 shikimate kinase AroK -
  MUK77_RS01705 (MUK77_01705) aroB 373818..374906 (+) 1089 WP_000439850.1 3-dehydroquinate synthase -
  MUK77_RS01710 (MUK77_01710) damX 374998..376284 (+) 1287 WP_000343173.1 cell division protein DamX -
  MUK77_RS01715 (MUK77_01715) dam 376391..377227 (+) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44811.29 Da        Isoelectric Point: 5.9488

>NTDB_id=581982 MUK77_RS01695 WP_000816009.1 371601..372839(+) (comE) [Escherichia coli isolate EC5654]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNVARQEVEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQGGGVGQVTTLGSDLSVATATTHIGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=581982 MUK77_RS01695 WP_000816009.1 371601..372839(+) (comE) [Escherichia coli isolate EC5654]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTTATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTTTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATTTAACTGACGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGATTG
ATAACGCGCCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATGTCGCCCGCCAGGAAGTGGA
GCAGACGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGTTGGCCGATGCGCAACAAGGTGGTGGCGTTG
GGCAAGTCACTACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATATCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTAGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.385

100

0.396
  pilQ Vibrio campbellii strain DS40M4

38.48

100

0.393
  comE Haemophilus influenzae 86-028NP

37.156

100

0.393
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  pilQ Pseudomonas aeruginosa PAK

35.092

100

0.371
  comE Glaesserella parasuis strain SC1401

35.782

100

0.367