Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KSP27_RS00475 Genome accession   NZ_CP077603
Coordinates   104268..106577 (-) Length   769 a.a.
NCBI ID   WP_002221480.1    Uniprot ID   A0AAD2J7E1
Organism   Neisseria meningitidis strain S4     
Function   pseudopilus extrusion and retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 99268..111577
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KSP27_RS00460 - 99503..100345 (-) 843 WP_002224653.1 class II glutamine amidotransferase -
  KSP27_RS00465 aroB 101635..102714 (-) 1080 WP_002221481.1 3-dehydroquinate synthase -
  KSP27_RS00470 - 102794..103306 (-) 513 WP_002214526.1 shikimate kinase -
  KSP27_RS00475 pilQ 104268..106577 (-) 2310 WP_002221480.1 type IV pilus secretin PilQ Machinery gene
  KSP27_RS00480 pilP 106596..107141 (-) 546 WP_002214522.1 pilus assembly protein PilP Machinery gene
  KSP27_RS00485 - 107159..107806 (-) 648 WP_002217908.1 type 4a pilus biogenesis protein PilO -
  KSP27_RS00490 pilN 107807..108406 (-) 600 WP_002221479.1 PilN domain-containing protein Machinery gene
  KSP27_RS00495 pilM 108409..109524 (-) 1116 WP_002221478.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 769 a.a.        Molecular weight: 82522.17 Da        Isoelectric Point: 10.1701

>NTDB_id=581558 KSP27_RS00475 WP_002221480.1 104268..106577(-) (pilQ) [Neisseria meningitidis strain S4]
MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKVSFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVL
EYADPLLSKISAAQNSSRARLVLNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAAAPAKQQAAAPAKQTNIDFRKDGKNAGII
ELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKTPVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYF
TFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGS
VLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGF
GGDDKWGAETKINLPITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIPFTVTSIAN
GGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTILCISTKNLNTQAMVENGGTLIVGGIYEEDNG
NTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFITPRIMGTAGNSLRY

Nucleotide


Download         Length: 2310 bp        

>NTDB_id=581558 KSP27_RS00475 WP_002221480.1 104268..106577(-) (pilQ) [Neisseria meningitidis strain S4]
ATGAATACCAAACTGACAAAAATCATTTCCGGTCTCTTTGTCGCAACCGCCGCCTTTCAGACAGCATCGGCAGGAAACAT
TACAGACATCAAAGTTTCCTCCCTGCCCAACAAACAGAAAATCGTCAAAGTCAGCTTTGACAAAGAGATTGTCAACCCGA
CCGGCTTCGTAACCTCCTCACCGGCCCGCATCGCCTTGGACTTTGAACAAACCGGCATTTCCATGGATCAACAGGTACTC
GAATATGCCGATCCTCTGTTGAGCAAAATCAGTGCCGCACAAAACAGCAGCCGTGCGCGTCTGGTTCTGAATCTGAACAA
ACCGGGCCAATACAATACCGAAGTACGCGGGAACAAAGTTTGGATATTCATTAACGAATCGGACGATACCGTGTCCGCCC
CCGCACGCCCCGCCGTAAAAGCCGCGCCTGCCGCACCGGCAAAACAACAGGCTGCCGCACCGTCTACCAAGTCCGCAGTA
TCCGTATCCAAACCCTTTACCCCGGCAAAACAACAGGCTGCCGCACCGTTTACCGAGTCCGTAGTATCCGTATCCGCACC
GTTCAGCCCGGCAAAACAACAGGCGGCGGCATCAGCAAAACAACAGACGGCAGCACCAGCAAAACAACAGGCGGCAGCAC
CAGCAAAACAACAGGCGGCAGCACCAGCAAAACAAACCAATATCGATTTCCGCAAAGACGGCAAAAATGCCGGCATTATC
GAATTGGCTGCATTGGGCTTTGCCGGGCAGCCCGACATCAGCCAACAGCACGACCACATCATCGTTACGCTGAAAAACCA
TACCCTGCCGACCACGCTCCAACGCAGTTTGGATGTGGCAGACTTTAAAACACCGGTTCAAAAGGTTACGCTGAAACGCC
TCAATAACGACACCCAGCTGATTATCACAACAGCCGGCAACTGGGAACTCGTCAACAAATCCGCCGCGCCCGGATACTTT
ACCTTCCAAGTCCTGCCGAAAAAACAAAACCTCGAGTCAGGCGGCGTGAACAATGCGCCCAAAACCTTCACAGGCCGGAA
AATCTCCCTTGACTTCCAAGATGTCGAAATCCGCACCATCCTGCAGATTTTGGCAAAAGAATCCGGGATGAACATTGTTG
CCAGCGACTCCGTCAACGGCAAAATGACCCTCTCCCTCAAAGACGTACCTTGGGATCAGGCTTTGGATTTGGTTATGCAG
GCACGCAACCTCGATATGCGCCAACAAGGGAACATCGTCAACATCGCGCCCCGCGACGAGCTGCTTGCCAAAGACAAAGC
CTTCTTACAGGCGGAAAAAGACATTGCCGATCTGGGCGCGCTGTATTCACAAAACTTCCAATTGAAATACAAAAATGTGG
AAGAATTCCGCAGCATCCTGCGTTTGGACAATGCCGACACAACCGGAAACCGCAATACGCTTGTCAGCGGCAGGGGCAGC
GTGCTGATCGATCCCGCCACCAATACCCTGATTGTTACCGATACCCGCAGCGTCATCGAAAAATTCCGCAAACTGATTGA
CGAATTGGACGTACCCGCGCAACAAGTGATGATTGAGGCGCGTATCGTCGAAGCGGCAGACGGCTTCTCGCGCGATTTGG
GCGTTAAATTCGGCGCGACAGGCAAGAAAAAGCTGAAAAATGATACAAGCGCATTCGGCTGGGGGGTAAACTCCGGCTTC
GGCGGCGACGATAAATGGGGGGCCGAAACCAAAATCAACCTGCCGATTACCGCTGCCGCAAACAGCATTTCGCTGGTGCG
CGCGATTTCCTCCGGTGCCTTGAATTTGGAATTGTCCGCATCCGAATCGCTTTCAAAAACCAAAACGCTTGCCAATCCGC
GCGTGCTGACCCAAAACCGCAAAGAGGCCAAAATCGAATCCGGTTACGAAATTCCTTTCACCGTAACCTCAATCGCGAAC
GGCGGCAGCAGCACGAACACGGAACTCAAAAAAGCCGTCTTGGGGCTGACCGTTACGCCGAACATCACGCCCGACGGCCA
AATCATTATGACCGTCAAAATCAACAAGGACTCGCCTGCGCAATGTGCCTCCGGTAATCAGACGATCCTGTGTATTTCGA
CCAAAAACCTGAATACGCAGGCTATGGTTGAAAACGGCGGCACATTGATTGTCGGCGGTATTTATGAAGAAGACAACGGC
AATACGCTGACCAAAGTCCCCCTGTTGGGCGACATCCCCGTTATCGGCAACCTCTTTAAAACACGCGGGAAAAAAACCGA
CCGCCGCGAACTGCTGATTTTCATTACCCCGAGGATTATGGGTACGGCCGGCAACAGCCTGCGCTATTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(444-515)

Bacterial type II and III secretion system protein (Secretin)

(601-760)

Secretin and TonB N terminus short domain (STN)

(371-416)

AMIN domain (AMIN)

(43-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Neisseria meningitidis MC58

99.48

100

0.995
  pilQ Neisseria meningitidis M1080

98.57

100

0.986
  pilQ Neisseria gonorrhoeae strain FA1090

91.417

100

0.914