Detailed information NTG0057934

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: SPN994039_RS04580
• Genome accession: NC_021005
• Coordinates: 857475..858125 (+)
• Length: 216 a.a.
• NCBI ID: WP_000387335.1
• Uniprot ID: A0A0B7MF65
• Organism: Streptococcus pneumoniae SPN994039
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 852475..863125

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPN994039_RS04540 (SPN994039_08390)	-	852959..853174 (+)	216	WP_001232082.1	YozE family protein	-
SPN994039_RS04545 (SPN994039_08400)	-	853260..854228 (+)	969	WP_000658183.1	PhoH family protein	-
SPN994039_RS04550 (SPN994039_08410)	-	854421..854921 (+)	501	WP_000566982.1	GNAT family N-acetyltransferase	-
SPN994039_RS04555 (SPN994039_08420)	-	854924..855249 (+)	326	Protein_880	TfoX/Sxy family protein	-
SPN994039_RS12990 (SPN994039_08430)	ald	855550..856661 (-)	1112	Protein_881	alanine dehydrogenase	-
SPN994039_RS04575 (SPN994039_08440)	-	856838..857407 (+)	570	WP_000443774.1	GNAT family N-acetyltransferase	-
SPN994039_RS04580 (SPN994039_08450)	comEA/celA/cilE	857475..858125 (+)	651	WP_000387335.1	helix-hairpin-helix domain-containing protein	Machinery gene
SPN994039_RS04585 (SPN994039_08460)	comEC/celB	858109..860349 (+)	2241	WP_000942414.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
SPN994039_RS04590	-	860528..860716 (+)	189	WP_001809102.1	hypothetical protein	-
SPN994039_RS04595 (SPN994039_08470)	-	860748..861335 (+)	588	WP_000933542.1	ABC transporter ATP-binding protein	-
SPN994039_RS04600 (SPN994039_08480)	-	861339..862514 (+)	1176	WP_000655957.1	ABC transporter permease	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23229.61 Da
• Isoelectric Point: 5.2344

>NTDB_id=57934 SPN994039_RS04580 WP_000387335.1 857475..858125(+) (comEA/celA/cilE) [Streptococcus pneumoniae SPN994039]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTNGEEAVSQQTGLGTASSISKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=57934 SPN994039_RS04580 WP_000387335.1 857475..858125(+) (comEA/celA/cilE) [Streptococcus pneumoniae SPN994039]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGCGGATT
TTTCCTGCTAAAACCAGCTCCACAAACACCTGTCAAAGAGACGAATTTGCAGGCTGAAGTCGCAGCTGTTTCCAAGGATT
TGGTATCCGAAAAGGAAGTGAACAAGGAAGAAAAGGAAGAACCCCTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAATGGAG
AAGAAGCAGTTAGCCAACAGACTGGTTTGGGGACAGCTTCTTCAATAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCT
AGTCTGGAAGAACTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATCGACCATCGTGAGGCAAATGG
CAAGTTCAAATCAGTAGACGAGCTCAAGAAGGTCTCTGGCATTGGTGCCAAGACTATAGAAAAGTTAAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (151-214)

• SLBB domain (SLBB) (76-126)

3D structure

• AlphaFold DB: A0A0B7MF65

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae Rx1	99.074	100	0.991
comEA/celA/cilE	Streptococcus pneumoniae D39	99.074	100	0.991
comEA/celA/cilE	Streptococcus pneumoniae R6	99.074	100	0.991
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	96.296	100	0.963
comEA/celA/cilE	Streptococcus mitis NCTC 12261	96.296	100	0.963
comEA/celA/cilE	Streptococcus mitis SK321	90.278	100	0.903
comEA	Lactococcus lactis subsp. cremoris KW2	43.172	100	0.454
comEA	Bacillus subtilis subsp. subtilis str. 168	41.053	87.963	0.361