Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HU739_RS15550 Genome accession   NZ_CP077091
Coordinates   3605922..3606986 (-) Length   354 a.a.
NCBI ID   WP_186549543.1    Uniprot ID   -
Organism   Pseudomonas hamedanensis strain SWRI65     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3600922..3611986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU739_RS15525 (HU739_015525) aroK 3601597..3602115 (-) 519 WP_186549533.1 shikimate kinase AroK -
  HU739_RS15530 (HU739_015530) pilQ 3602120..3604198 (-) 2079 WP_186549535.1 type IV pilus secretin PilQ Machinery gene
  HU739_RS15535 (HU739_015535) - 3604212..3604739 (-) 528 WP_186549537.1 pilus assembly protein PilP -
  HU739_RS15540 (HU739_015540) pilO 3604736..3605359 (-) 624 WP_186549539.1 type 4a pilus biogenesis protein PilO -
  HU739_RS15545 (HU739_015545) - 3605356..3605922 (-) 567 WP_186549541.1 PilN domain-containing protein -
  HU739_RS15550 (HU739_015550) pilM 3605922..3606986 (-) 1065 WP_186549543.1 pilus assembly protein PilM Machinery gene
  HU739_RS15555 (HU739_015555) - 3607206..3609650 (+) 2445 WP_407681961.1 penicillin-binding protein 1A -
  HU739_RS15560 (HU739_015560) - 3609958..3610908 (+) 951 WP_186552637.1 transposase -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38033.65 Da        Isoelectric Point: 5.0031

>NTDB_id=579078 HU739_RS15550 WP_186549543.1 3605922..3606986(-) (pilM) [Pseudomonas hamedanensis strain SWRI65]
MLGLFKKKTNALLGIDISSTSVKLLELSRHGNRYRVEAYAVEPLPASAVVEKNIAELEGVGQALSRVLVKARTGLKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVSVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYISEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLNTPTQVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=579078 HU739_RS15550 WP_186549543.1 3605922..3606986(-) (pilM) [Pseudomonas hamedanensis strain SWRI65]
GTGCTGGGACTCTTCAAGAAAAAGACCAACGCGTTACTGGGCATCGACATCAGTTCCACTTCGGTGAAGCTGCTGGAACT
GAGCCGCCACGGCAATCGCTACCGGGTCGAGGCCTATGCGGTGGAGCCGTTGCCGGCCAGTGCCGTGGTCGAAAAGAACA
TCGCCGAACTCGAGGGCGTGGGCCAGGCCCTGAGCCGGGTGCTGGTCAAGGCGCGTACGGGTCTCAAAAGCGTGGCGGTG
GCGGTGGCCGGTTCGGCGGTCATCACCAAGGTCATCGAGATGGACGCCGGGCTGTCCGACGACGAGCTGGAAAACCAGCT
GAAGATCGAAGCCGACCAATACATTCCCTATCCGCTCGATGAAGTCGCGATCGACTTCGAAGTCCAGGGCGTGTCGCCGC
GCAACCCGGAACGGGTCAGCGTGCTGTTGGCCGCCTGTCGCAAGGAAAACGTCGAGGTGCGTGAAGCAGCGCTGGCGCTT
GCCGGGCTGACCGCGCGCGTGGTGGACGTTGAAGCCTATGCGCTGGAGCGCTCGTTCGGCCTGCTGGCGACCCAACTGGC
CGCCTCCCAGGAACGCCTCACGGTCGCGGTGGTGGACATCGGTGCGACCATGACCACGCTCAGTGTCCTGCACAACGGCA
AGATCATTTATACCCGCGAGCAATTGTTCGGCGGCCGCCAGCTCACCGAAGAGATCCAGCGCCGCTACGGCCTGACCGTC
GAGCAGGCGGGGCTGGCGAAAAAGCAGGGCGGGCTGCCCGACGATTACATCAGCGAGGTCTTGCAACCGTTTCGCGAGGC
GCTGGTACAGCAAGTCTCTCGTTCGTTGCAGTTCTTCTTCGCTTCCGGCCAGTACAACGCGGTCGACCATATTCTGCTGG
CCGGCGGCACGGCCTCGGTGCCCGGCCTGGATCGCCTGATCGAACAGCGCCTGAACACCCCGACCCAGGTCGCCAACCCG
TTTGCCGACATGGCCCTGAGCAGCAAGGTCAATGCCGGGGCGCTGGCCAGCGACGCGCCGGCCCTGATGATTGCTTGCGG
GCTCGCGCTCAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

48.023

100

0.48