Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HU718_RS03050 Genome accession   NZ_CP077089
Coordinates   691627..692691 (+) Length   354 a.a.
NCBI ID   WP_150692970.1    Uniprot ID   -
Organism   Pseudomonas tensinigenes strain ZA 5.3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 686627..697691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU718_RS03040 (HU718_003040) - 687375..688643 (+) 1269 WP_102899524.1 malic enzyme-like NAD(P)-binding protein -
  HU718_RS03045 (HU718_003045) - 688963..691410 (-) 2448 WP_150728843.1 penicillin-binding protein 1A -
  HU718_RS03050 (HU718_003050) pilM 691627..692691 (+) 1065 WP_150692970.1 pilus assembly protein PilM Machinery gene
  HU718_RS03055 (HU718_003055) - 692691..693257 (+) 567 WP_095120724.1 PilN domain-containing protein -
  HU718_RS03060 (HU718_003060) pilO 693254..693877 (+) 624 WP_150707956.1 type 4a pilus biogenesis protein PilO -
  HU718_RS03065 (HU718_003065) - 693874..694401 (+) 528 WP_186612996.1 pilus assembly protein PilP -
  HU718_RS03070 (HU718_003070) pilQ 694415..696493 (+) 2079 WP_186612994.1 type IV pilus secretin PilQ family protein Machinery gene
  HU718_RS03075 (HU718_003075) aroK 696498..697016 (+) 519 WP_011332081.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38136.77 Da        Isoelectric Point: 4.9006

>NTDB_id=579013 HU718_RS03050 WP_150692970.1 691627..692691(+) (pilM) [Pseudomonas tensinigenes strain ZA 5.3]
MLGLFNKKAKTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPVNAVVEKNIAELEGVGQALSRVLVKARTGLKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYISEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLNTPTQVANP
FSNMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=579013 HU718_RS03050 WP_150692970.1 691627..692691(+) (pilM) [Pseudomonas tensinigenes strain ZA 5.3]
GTGCTGGGACTCTTCAATAAAAAGGCCAAAACGTTACTGGGGATCGACATCAGCTCCACGTCAGTGAAGCTGCTGGAACT
GAGCCGTCAGGGTGACCGCTACCGGGTCGAGGCATACGCGGTAGAGCCATTGCCGGTCAATGCCGTGGTCGAAAAGAACA
TCGCCGAGCTTGAAGGCGTCGGTCAGGCCCTGAGCCGCGTGCTGGTCAAGGCGCGTACCGGGCTCAAGAGCGTGGCGGTG
GCGGTGGCCGGTTCCGCCGTGATCACCAAGGTCATCGAAATGGACGCCGGGCTGTCCGACGATGAACTGGAAAACCAGCT
CAAGATCGAAGCCGACCAATACATTCCCTACCCGCTGGACGAAGTCGCCATCGACTTTGAAGTCCAGGGCGTATCACCGC
GCAATCCCGAGCGGGTCAACGTGTTGCTCGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGTGAAGCGGCGCTGGCTCTC
GCCGGGCTGACCGCGCGGGTGGTCGATGTCGAGGCTTATGCGCTGGAGCGCTCGTTCGGCTTGCTCGCAACGCAACTGGC
AGCCTCTCAGGAGCGTCTGACCGTGGCGGTGGTCGACATCGGCGCGACCATGACCACCCTCAGCGTCCTGCACAACGGCA
AGATCATCTATACCCGCGAGCAACTGTTCGGGGGGCGGCAATTGACCGAAGAAATCCAGCGGCGTTATGGCCTGACCCTC
GAGCAAGCGGGGCTGGCGAAAAAGCAGGGTGGTCTGCCCGATGATTACATCAGTGAGGTCTTGCAGCCGTTCCGTGAGGC
GCTGGTGCAGCAGGTCTCACGCTCATTGCAGTTCTTCTTCGCCTCCGGGCAATACAACGCCGTCGATCACATTCTGCTCG
CCGGTGGCACGGCGTCAGTGCCTGGGCTGGACCGGCTGATCGAGCAACGCCTGAACACGCCGACGCAAGTGGCCAACCCG
TTTTCCAATATGGCCTTGAGCAGCAAGGTCAACGCCGGTGCGCTGGCCAGCGATGCGCCGGCGCTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

57.062

100

0.571
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

47.74

100

0.477