Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HU718_RS03070 Genome accession   NZ_CP077089
Coordinates   694415..696493 (+) Length   692 a.a.
NCBI ID   WP_186612994.1    Uniprot ID   -
Organism   Pseudomonas tensinigenes strain ZA 5.3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 689415..701493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU718_RS03050 (HU718_003050) pilM 691627..692691 (+) 1065 WP_150692970.1 pilus assembly protein PilM Machinery gene
  HU718_RS03055 (HU718_003055) - 692691..693257 (+) 567 WP_095120724.1 PilN domain-containing protein -
  HU718_RS03060 (HU718_003060) pilO 693254..693877 (+) 624 WP_150707956.1 type 4a pilus biogenesis protein PilO -
  HU718_RS03065 (HU718_003065) - 693874..694401 (+) 528 WP_186612996.1 pilus assembly protein PilP -
  HU718_RS03070 (HU718_003070) pilQ 694415..696493 (+) 2079 WP_186612994.1 type IV pilus secretin PilQ family protein Machinery gene
  HU718_RS03075 (HU718_003075) aroK 696498..697016 (+) 519 WP_011332081.1 shikimate kinase AroK -
  HU718_RS03080 (HU718_003080) aroB 697169..698269 (+) 1101 WP_186612992.1 3-dehydroquinate synthase -
  HU718_RS03085 (HU718_003085) - 698280..699872 (+) 1593 WP_102899518.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75066.57 Da        Isoelectric Point: 5.8300

>NTDB_id=579014 HU718_RS03070 WP_186612994.1 694415..696493(+) (pilQ) [Pseudomonas tensinigenes strain ZA 5.3]
MNRIFSTLGFSLWIALMSPMVFAANLKTLDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLASKNLD
LGSGNARTATVVEAKERTRLIVSLTQLAPYTTRVEGNNLFVVVGQGAPAAATRPAAVAPRATAKAPAPAKAFVPKNRAIR
GVDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILNFTRTQLPDKLRVRLDVKDFATPVQFVNAGVSGDRTVITVEPSG
TYEYSTFQTDNKLTVSIRPMTVDDLQKRNADRQAYVGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNASGVN
GSSTTIGTPGSTSTNSPFVDMGAVGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=579014 HU718_RS03070 WP_186612994.1 694415..696493(+) (pilQ) [Pseudomonas tensinigenes strain ZA 5.3]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGATGTCGCCGATGGTATTCGCGGCCAATCTGAA
GACGCTCGACGTGGCGGCGTTGCCGGGTGATCGCGTCGAGCTGAAGTTGTCGTTCGACGGCCCGCCGCCGCAACCCAAGG
GCTACACCACCGAATCACCGGCGCGGATTGCGCTGGATCTGCCCGGTGTCGCCAGTCAGTTGGCCAGCAAGAATCTTGAT
CTGGGCAGTGGTAATGCGCGCACGGCCACGGTGGTCGAGGCCAAGGAGCGGACGCGGCTGATTGTCAGTCTCACGCAACT
GGCGCCTTACACCACGCGGGTCGAAGGCAATAACCTGTTCGTGGTGGTTGGTCAGGGCGCACCGGCAGCGGCGACGCGTC
CTGCTGCCGTCGCACCTCGTGCTACCGCTAAAGCGCCTGCGCCCGCCAAGGCCTTTGTGCCGAAAAACCGGGCGATTCGC
GGTGTGGACTTCCAGCGCGGTACGGCAGGTGAAGGTAATGTCGTCATCGACCTGTCCGACCCGACCATCGCTCCGGATAT
CCAGGAACATGACGGCAAGATCATCCTCAACTTTACCCGCACGCAGTTACCAGACAAGTTGCGGGTACGCCTCGACGTCA
AGGATTTTGCTACTCCGGTGCAGTTCGTCAATGCCGGGGTGAGCGGTGATCGCACGGTTATTACTGTCGAACCCAGCGGC
ACCTACGAGTACTCCACCTTCCAGACCGACAACAAGCTAACCGTCAGCATCCGCCCGATGACCGTCGATGACCTGCAGAA
ACGTAATGCCGACCGTCAGGCGTACGTCGGCGAAAAGCTCTCGCTGAATTTCCAGGACATTGATGTGCGCTCGGTGCTGC
AACTGATCGCCGATTTCACCAACCTCAATCTGGTCGCCAGCGATACGGTGCAGGGCGGCATCACCTTGCGTCTGCAAAAC
GTGCCGTGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAAGGTCTGGATAAACGCAAGATCGGTAACGTGCTGCTGGT
CGCTCCCGCCGATGAAATCGCTGCCCGCGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATTGCCGAACTGGCGCCGTTGC
GCCGTGAGCTGCTGCAAGTGAATTACGCCAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGTGCTGAGGCT
AAAATCGACGAGCGCGGTTCGATTACTGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACTCAGGATCGTCTCGA
CGAACTGCGGCGGATCGTGGCGCAGCTGGATATTCCGGTGCGTCAGGTGATGATCGAGGCGCGGATCGTCGAGGCTAACG
TCGATTACGACAAAAGCCTCGGCGTGCGCTGGGGCGGCTCGATCCAGAACAAGGGCAACTGGAACGCTTCCGGGGTCAAC
GGTTCATCAACCACCATTGGTACGCCGGGCAGCACCAGTACCAACTCACCGTTTGTCGATATGGGGGCCGTGGGTAATAC
CTCGGGGATCGGCATTGCCTTCATCACTGACAATGTCTTGCTCGATCTTGAGCTGACAGCGATGGAGAAAACCGGCAACG
GCGAAATCGTCTCGCAGCCGAAAGTGGTCACCTCCGACAAAGAAACCGCAAAAATCCTCAAAGGCACCGAGATTCCGTAT
CAGGAAGCCAGCTCCAGCGGTGCCACGTCGGTGTCGTTCAAGGAGGCATCGCTGTCGCTGGAAGTCACGCCGCAGATCAC
CCCCGACAATCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGATTACCTGAACAAAGTGCAGGATGTGCCGC
CGATCAAGAAAAACGAGGTCAACGCCAAGGTACTGGTCAATGACGGCGAAACCATCGTGATTGGCGGTGTTTTCTCAAAT
ACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACACCACGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(37-122)

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.147

100

0.756
  comQ Acinetobacter baylyi ADP1

42.385

100

0.426
  pilQ Acinetobacter baumannii strain A118

38.462

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.462

100

0.412
  pilQ Legionella pneumophila strain Lp02

38.201

97.977

0.374
  pilQ Legionella pneumophila strain ERS1305867

38.166

97.688

0.373