Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HV782_RS03065 Genome accession   NZ_CP077087
Coordinates   705883..706947 (+) Length   354 a.a.
NCBI ID   WP_123470753.1    Uniprot ID   -
Organism   Pseudomonas monsensis strain PGSB 8459     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 700883..711947
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV782_RS03055 (HV782_003055) - 701357..702625 (+) 1269 WP_003220889.1 malic enzyme-like NAD(P)-binding protein -
  HV782_RS03060 (HV782_003060) - 703219..705666 (-) 2448 WP_186746224.1 penicillin-binding protein 1A -
  HV782_RS03065 (HV782_003065) pilM 705883..706947 (+) 1065 WP_123470753.1 pilus assembly protein PilM Machinery gene
  HV782_RS03070 (HV782_003070) - 706947..707513 (+) 567 WP_128615714.1 PilN domain-containing protein -
  HV782_RS03075 (HV782_003075) pilO 707510..708133 (+) 624 WP_186746221.1 type 4a pilus biogenesis protein PilO -
  HV782_RS03080 (HV782_003080) - 708130..708657 (+) 528 WP_123470758.1 pilus assembly protein PilP -
  HV782_RS03085 (HV782_003085) pilQ 708671..710752 (+) 2082 WP_186746219.1 type IV pilus secretin PilQ Machinery gene
  HV782_RS03090 (HV782_003090) aroK 710757..711275 (+) 519 WP_045122260.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38151.74 Da        Isoelectric Point: 4.8182

>NTDB_id=578985 HV782_RS03065 WP_123470753.1 705883..706947(+) (pilM) [Pseudomonas monsensis strain PGSB 8459]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPANAVVEKNIAELEGVGHALSRVLVKARTGLKSVAV
AVAGSAVITKIIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVSVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAVSQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLNTPTQVANP
FFDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=578985 HV782_RS03065 WP_123470753.1 705883..706947(+) (pilM) [Pseudomonas monsensis strain PGSB 8459]
GTGCTGGGACTCTTCAATAAAAAGGCCAATACGTTACTGGGGATCGACATCAGCTCCACTTCGGTGAAGTTGCTGGAACT
GAGCCGTCAGGGTGATCGCTACCGGGTCGAAGCGTACGCGGTGGAGCCGTTGCCAGCGAATGCCGTGGTCGAGAAAAACA
TCGCCGAACTCGAAGGCGTCGGGCACGCCCTGAGCCGGGTGCTGGTCAAGGCGCGCACCGGGCTCAAGAGCGTGGCCGTG
GCCGTGGCCGGTTCAGCGGTGATCACCAAGATCATCGAAATGGATGCCGGACTGTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATTCCCTACCCGCTGGACGAAGTGGCCATCGACTTCGAAGTCCAGGGCGTGTCCCCGC
GCAATCCCGAGCGCGTCAGCGTGCTGCTCGCCGCCTGTCGCAAGGAAAACGTCGAAGTTCGCGAGGCGGCACTGGCCCTC
GCCGGGCTGACGGCGCGCGTGGTGGATGTCGAGGCCTACGCGCTGGAGCGCTCGTTCGGCCTGCTTGCCACGCAACTGGC
GGTGTCCCAGGAGCGTCTGACCGTGGCGGTGGTCGACATCGGCGCGACCATGACCACCCTCAGCGTCCTGCACAACGGCA
AGATCATTTACACCCGCGAGCAATTGTTCGGCGGCCGGCAACTGACCGAGGAAATCCAGCGGCGTTACGGGCTGACGGTC
GAACAGGCCGGGTTGGCGAAAAAGCAGGGCGGACTGCCCGACGATTATGTCAGCGAGGTCTTGCAGCCGTTTCGCGAGGC
ACTGGTACAGCAGGTGTCGCGCTCGTTGCAGTTCTTTTTCGCTTCCGGCCAGTACAACGCGGTCGATCACATTTTATTGG
CCGGCGGCACGGCGTCGGTGCCGGGCCTGGATCGTTTGATCGAGCAGCGCCTGAACACCCCGACCCAGGTCGCCAACCCG
TTTTTCGACATGGCCCTGAGCAGCAAGGTCAACGCCGGGGCCCTGGCCAGTGATGCGCCGGCTCTGATGATTGCCTGCGG
GCTCGCGCTGCGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

55.367

100

0.554
  pilM Legionella pneumophila strain ERS1305867

46.893

100

0.469