Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   KSS92_RS02875 Genome accession   NZ_CP077081
Coordinates   658459..659523 (+) Length   354 a.a.
NCBI ID   WP_217879175.1    Uniprot ID   -
Organism   Pseudomonas atacamensis strain SWRI76     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 653459..664523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KSS92_RS02865 (KSS92_02865) - 654206..655474 (+) 1269 WP_016772053.1 malic enzyme-like NAD(P)-binding protein -
  KSS92_RS02870 (KSS92_02870) - 655795..658239 (-) 2445 WP_404822219.1 penicillin-binding protein 1A -
  KSS92_RS02875 (KSS92_02875) pilM 658459..659523 (+) 1065 WP_217879175.1 pilus assembly protein PilM Machinery gene
  KSS92_RS02880 (KSS92_02880) - 659523..660089 (+) 567 WP_110602598.1 PilN domain-containing protein -
  KSS92_RS02885 (KSS92_02885) pilO 660086..660709 (+) 624 WP_217879176.1 type 4a pilus biogenesis protein PilO -
  KSS92_RS02890 (KSS92_02890) - 660706..661230 (+) 525 WP_024011268.1 pilus assembly protein PilP -
  KSS92_RS02895 (KSS92_02895) pilQ 661244..663322 (+) 2079 WP_217879177.1 type IV pilus secretin PilQ Machinery gene
  KSS92_RS02900 (KSS92_02900) aroK 663327..663845 (+) 519 WP_024011270.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38073.62 Da        Isoelectric Point: 4.7539

>NTDB_id=578829 KSS92_RS02875 WP_217879175.1 658459..659523(+) (pilM) [Pseudomonas atacamensis strain SWRI76]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIAELEGVGQALSRLLVKARTGVKSVAV
AVAGSAVITKVIEMDVGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATHLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLDTPTQVANP
FADMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=578829 KSS92_RS02875 WP_217879175.1 658459..659523(+) (pilM) [Pseudomonas atacamensis strain SWRI76]
GTGCTGGGACTCTTCAATAAAAAGACCAATGCGTTACTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTGGAACT
GAGCCGTCAGGGCGAGCGCTACCGGGTCGAGGCCTACGCGGTGGAGCCGTTGCCGGCGAACGCTGTGGTCGAAAAGAACA
TCGCCGAACTCGAGGGCGTTGGCCAGGCACTGAGCCGCCTGCTGGTCAAGGCGCGTACCGGGGTCAAGAGCGTGGCGGTG
GCAGTGGCGGGTTCGGCGGTGATCACCAAGGTCATCGAGATGGACGTCGGGCTGTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAGGCCGACCAATACATTCCCTATCCGCTGGACGAAGTCGCCATCGACTTTGAAGTCCAGGGCGTCTCGCCGC
GCAACCCGGAACGGGTCAACGTGCTGCTTGCCGCCTGTCGCAAGGAAAACGTCGAGGTGCGTGAAGCGGCGCTGGCGCTC
GCCGGCCTGACCGCGCGGGTGGTGGATGTCGAGGCCTACGCGCTGGAGCGCTCGTTCGGCCTGCTGGCGACGCATCTGGC
GGCCTCACAGGAGCGCCTGACCGTGGCGGTGGTCGACATTGGCGCGACCATGACCACTCTCAGCGTCCTGCACAACGGCA
AGATCATCTATACCCGCGAGCAATTGTTTGGCGGGCGCCAGCTCACCGAAGAAATCCAGCGCCGCTACGGCCTCACGGTC
GAACAGGCGGGCCTGGCGAAAAAACAGGGCGGGCTGCCTGACGATTACGTCAGCGAGGTCTTGCAGCCATTTCGCGAGGC
GCTGGTGCAGCAAGTCTCGCGCTCGCTGCAGTTCTTCTTCGCCTCCGGCCAGTACAACGCGGTCGACCATATCCTGCTGG
CGGGCGGCACCGCTTCGGTGCCCGGCCTGGACCGTCTGATCGAACAGCGTCTGGACACGCCGACGCAGGTCGCCAACCCG
TTCGCCGACATGGCTTTAGGCAGCAAGGTCAATGCTGGGGCGCTGGCCAGTGATGCGCCAGCCTTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483