Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KM146_RS18260 Genome accession   NZ_CP076287
Coordinates   2263546..2264019 (+) Length   157 a.a.
NCBI ID   WP_065274839.1    Uniprot ID   A0A7Y4EZC0
Organism   Vibrio alginolyticus strain ZJ-0     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2258546..2269019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KM146_RS18245 (KM146_18245) pdhR 2260579..2261346 (-) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  KM146_RS18250 (KM146_18250) ampD 2261752..2262303 (-) 552 WP_005379985.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KM146_RS18255 (KM146_18255) nadC 2262396..2263283 (+) 888 WP_005379983.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KM146_RS18260 (KM146_18260) pilA 2263546..2264019 (+) 474 WP_065274839.1 pilin Machinery gene
  KM146_RS18265 (KM146_18265) pilB 2264025..2265710 (+) 1686 WP_065274840.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KM146_RS18270 (KM146_18270) pilC 2265734..2266957 (+) 1224 WP_017635918.1 type II secretion system F family protein Machinery gene
  KM146_RS18275 (KM146_18275) pilD 2267022..2267891 (+) 870 WP_064354291.1 prepilin peptidase Machinery gene
  KM146_RS18280 (KM146_18280) coaE 2267892..2268506 (+) 615 WP_065274841.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16576.11 Da        Isoelectric Point: 7.6275

>NTDB_id=574404 KM146_RS18260 WP_065274839.1 2263546..2264019(+) (pilA) [Vibrio alginolyticus strain ZJ-0]
MKNSKQKKQQGFTLIELMIVVAIIGILSAFAVPAYQNYTKKATLSEFPKAAAAAKLAVELCAHENASNETTFISECVKEE
NGIPAKFVLNNIEISVVKATDASVSGAIAVIAAADEVKGPIAKGEQYIMAASYKTQGLEWGASCKAGGKAQTDYCPN

Nucleotide


Download         Length: 474 bp        

>NTDB_id=574404 KM146_RS18260 WP_065274839.1 2263546..2264019(+) (pilA) [Vibrio alginolyticus strain ZJ-0]
ATGAAAAACAGTAAACAGAAAAAACAACAGGGTTTTACTCTGATTGAGTTAATGATTGTGGTGGCGATTATTGGCATCTT
ATCTGCTTTCGCAGTTCCTGCTTACCAAAATTATACTAAGAAAGCGACATTGTCAGAATTTCCAAAAGCAGCTGCGGCAG
CCAAATTAGCAGTAGAATTATGTGCCCATGAGAATGCCTCAAATGAAACAACATTTATTAGTGAATGTGTAAAGGAAGAA
AATGGAATTCCCGCTAAATTCGTTTTGAATAATATTGAAATTAGCGTTGTTAAAGCCACAGATGCTAGCGTTTCTGGTGC
GATAGCAGTTATTGCAGCAGCTGATGAAGTTAAGGGACCTATTGCTAAAGGCGAGCAATATATCATGGCTGCATCATATA
AAACTCAAGGCTTAGAGTGGGGGGCTTCATGTAAAGCTGGCGGTAAAGCACAAACAGACTACTGCCCAAACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y4EZC0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

56.051

100

0.561
  pilA Haemophilus influenzae Rd KW20

42.361

91.72

0.389
  pilA Haemophilus influenzae 86-028NP

39.86

91.083

0.363