Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   MN088_RS11490 Genome accession   NZ_CP093413
Coordinates   2242597..2243670 (-) Length   357 a.a.
NCBI ID   WP_010906319.1    Uniprot ID   Q9CDT9
Organism   Lactococcus lactis strain AH1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2237597..2248670
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MN088_RS13565 - 2237794..2237916 (-) 123 WP_023164646.1 hypothetical protein -
  MN088_RS11450 (MN088_11450) - 2237913..2238095 (-) 183 WP_003130605.1 hypothetical protein -
  MN088_RS11455 (MN088_11455) - 2238111..2238800 (-) 690 WP_014570818.1 phage regulatory protein -
  MN088_RS11460 (MN088_11460) - 2238859..2239092 (-) 234 WP_014570819.1 transcriptional regulator -
  MN088_RS11465 (MN088_11465) - 2239299..2239679 (+) 381 WP_031561101.1 XRE family transcriptional regulator -
  MN088_RS11470 (MN088_11470) - 2239690..2240274 (+) 585 WP_058221714.1 hypothetical protein -
  MN088_RS11475 (MN088_11475) - 2240330..2240869 (+) 540 WP_023189015.1 PH domain-containing protein -
  MN088_RS11480 (MN088_11480) - 2240989..2242446 (+) 1458 WP_031561103.1 recombinase family protein -
  MN088_RS11485 (MN088_11485) - 2242443..2242583 (-) 141 WP_023164653.1 hypothetical protein -
  MN088_RS11490 (MN088_11490) comGB 2242597..2243670 (-) 1074 WP_010906319.1 competence type IV pilus assembly protein ComGB Machinery gene
  MN088_RS11495 (MN088_11495) comGA 2243564..2244502 (-) 939 WP_010906320.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 40330.51 Da        Isoelectric Point: 10.3000

>NTDB_id=572233 MN088_RS11490 WP_010906319.1 2242597..2243670(-) (comGB) [Lactococcus lactis strain AH1]
MSLGHQKIGIKKLISYLQMDISHLLKPKGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEQFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=572233 MN088_RS11490 WP_010906319.1 2242597..2243670(-) (comGB) [Lactococcus lactis strain AH1]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAAAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTACATTAAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTAGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACATTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCGTTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACAATTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCGGGAATATAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-348)

Type II secretion system (T2SS), protein F (T2SSF)

(41-159)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9CDT9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

83.578

95.518

0.798
  comGB Streptococcus gordonii str. Challis substr. CH1

53.959

95.518

0.515
  comGB Streptococcus mutans UA140

53.372

95.518

0.51
  comGB Streptococcus mutans UA159

53.079

95.518

0.507
  comGB/cglB Streptococcus mitis SK321

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae TIGR4

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae Rx1

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae D39

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae R6

51.045

93.838

0.479
  comGB/cglB Streptococcus mitis NCTC 12261

50.448

93.838

0.473