Detailed information NTG0572122

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KM132_RS12725
• Genome accession: NZ_CP076119
• Coordinates: 2620747..2621361 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain NZ4
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2615747..2626361

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KM132_RS12695 (KM132_12695)	rpsT	2616050..2616316 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
KM132_RS12700 (KM132_12700)	holA	2616279..2617374 (-)	1096	Protein_2482	DNA polymerase III subunit delta	-
KM132_RS12705 (KM132_12705)	-	2617414..2617565 (-)	152	Protein_2483	hypothetical protein	-
KM132_RS12710 (KM132_12710)	-	2617606..2617740 (+)	135	WP_003152870.1	YqzM family protein	-
KM132_RS12715 (KM132_12715)	comEC	2617759..2620110 (-)	2352	WP_109567080.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KM132_RS12720 (KM132_12720)	-	2620111..2620680 (-)	570	WP_003152868.1	ComE operon protein 2	-
KM132_RS12725 (KM132_12725)	comEA	2620747..2621361 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
KM132_RS12730 (KM132_12730)	comER	2621420..2622241 (+)	822	WP_109567081.1	late competence protein ComER	-
KM132_RS12735 (KM132_12735)	-	2622310..2623047 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
KM132_RS12740 (KM132_12740)	rsfS	2623044..2623400 (-)	357	WP_007408260.1	ribosome silencing factor	-
KM132_RS12745 (KM132_12745)	yqeK	2623418..2623978 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KM132_RS12750 (KM132_12750)	-	2623968..2624537 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
KM132_RS12755 (KM132_12755)	yhbY	2624548..2624838 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
KM132_RS12760 (KM132_12760)	aroE	2624832..2625674 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=572122 KM132_RS12725 WP_014418419.1 2620747..2621361(-) (comEA) [Bacillus velezensis strain NZ4]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=572122 KM132_RS12725 WP_014418419.1 2620747..2621361(-) (comEA) [Bacillus velezensis strain NZ4]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAAAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363