Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   KLP38_RS14640 Genome accession   NZ_CP076060
Coordinates   3072371..3074095 (+) Length   574 a.a.
NCBI ID   WP_215528586.1    Uniprot ID   -
Organism   Cupriavidus sp. EM10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3067371..3079095
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KLP38_RS14625 (KLP38_14625) ltrA 3069044..3070743 (+) 1700 Protein_2926 group II intron reverse transcriptase/maturase -
  KLP38_RS14630 (KLP38_14630) ispB 3070907..3071836 (+) 930 WP_215530413.1 octaprenyl diphosphate synthase -
  KLP38_RS14640 (KLP38_14640) pilF 3072371..3074095 (+) 1725 WP_215528586.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KLP38_RS14645 (KLP38_14645) - 3074247..3075510 (+) 1264 Protein_2929 type II secretion system F family protein -
  KLP38_RS14650 (KLP38_14650) - 3075526..3076419 (+) 894 WP_215528587.1 A24 family peptidase -
  KLP38_RS14655 (KLP38_14655) coaE 3076498..3077145 (+) 648 WP_215530414.1 dephospho-CoA kinase -
  KLP38_RS14660 (KLP38_14660) zapD 3077411..3078169 (+) 759 WP_215528588.1 cell division protein ZapD -
  KLP38_RS14665 (KLP38_14665) - 3078178..3078369 (+) 192 WP_066732392.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63018.78 Da        Isoelectric Point: 8.0623

>NTDB_id=571752 KLP38_RS14640 WP_215528586.1 3072371..3074095(+) (pilF) [Cupriavidus sp. EM10]
MTLGLALAQSRRIAPALLAQLEQSAREKKTQLIDEIIGSGTMSAHDVAIFAADKYQLPLLDLSQYNLNRVPPALAGNREF
HAHRLLPLGRRENRLIVAMSDPANQAGVEAIRQKMNLPVETVVVEHDKLMKHVRAAGEALGTLKNISPVTPTAQKMIEYD
PSAAAAARKGGPGNEIDDAPVVRFLQKLLNEAFNRGASDLHFEPFEFFYRIRFRVDGVLQEVARPPLDIRDKIATRIKVL
SRLDISEKRVPQDGRMKLLISVPKEKDSKETIEKAIDFRVSTLPTLFGEKIVMRILDSSTDKLDIDQLGYEPQQKALLLE
VIKRPYGMVLVTGPTGSGKTVSLYTFLNMLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAVLKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLARRLCSCKRASDLAPQVLL
DAGFTDADIDGSWKPYHPVGCERCNGSGYKGRCGIYQVMPISEAMQQIILSHGTALQIAEQARKEGVLSLREAGLQKVKQ
GITSLEEVLATTNS

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=571752 KLP38_RS14640 WP_215528586.1 3072371..3074095(+) (pilF) [Cupriavidus sp. EM10]
ATGACACTAGGTCTTGCGCTTGCCCAGAGCCGGCGGATCGCACCAGCGCTGCTCGCCCAGCTGGAGCAATCGGCACGGGA
AAAGAAAACGCAGCTGATCGACGAGATCATTGGCAGCGGCACGATGTCCGCCCACGACGTGGCGATCTTTGCCGCCGACA
AATACCAGCTGCCGCTGCTCGACCTGTCCCAGTACAACCTCAACCGCGTGCCGCCGGCGCTGGCCGGCAACCGCGAATTC
CACGCCCACCGGCTGCTGCCGCTGGGCCGGCGCGAGAACCGGTTGATCGTGGCGATGTCCGACCCCGCCAACCAGGCTGG
CGTCGAGGCGATCCGGCAGAAGATGAACCTGCCGGTGGAAACGGTCGTGGTCGAGCACGACAAGCTCATGAAGCATGTGC
GGGCGGCCGGCGAGGCGCTTGGCACGCTCAAGAACATCTCGCCGGTCACGCCGACCGCGCAGAAGATGATCGAGTACGAC
CCGTCGGCCGCGGCGGCTGCCCGCAAGGGTGGGCCGGGCAACGAGATCGATGACGCCCCGGTGGTGCGCTTCCTGCAGAA
GCTGCTGAACGAGGCCTTCAACCGCGGCGCGTCGGACTTGCACTTCGAACCGTTCGAATTCTTCTACCGTATCCGCTTCC
GCGTCGACGGCGTGCTGCAGGAGGTTGCCCGGCCGCCGCTGGACATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTG
TCGCGGCTGGACATCTCCGAAAAGCGCGTGCCGCAGGACGGCCGGATGAAGCTGCTGATCTCCGTGCCCAAGGAGAAGGA
CAGCAAGGAAACCATCGAGAAGGCCATCGACTTCCGGGTCTCGACCCTGCCGACGCTGTTTGGCGAGAAGATCGTGATGC
GGATCCTGGATTCGTCCACCGACAAGCTCGATATCGACCAGCTCGGCTACGAGCCCCAGCAGAAGGCACTGCTGCTCGAA
GTCATCAAGCGCCCGTACGGGATGGTGCTGGTCACCGGCCCGACCGGCAGCGGCAAGACCGTGTCCCTGTACACGTTCCT
GAACATGCTGAACCAGGGCGACATCAATATCTCCACGGCCGAGGACCCGGCGGAAATCCAGCTGCCGGGCATCAACCAGG
TGAACGTCAACGACAAGGCGGGCCTGACCTTCGCGGCCGTGCTCAAGTCGTTCCTGCGGCAGGATCCGGACATCATCATG
GTCGGCGAAATCCGCGACCTGGAAACCGCCGACATCTCGATCAAGGCCGCCCAGACGGGCCACCTGGTGCTGTCCACGCT
GCACACCAACGATGCGCCCACCACGCTGACGCGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCGTCGTCGGTGC
TGATGATCACCGCGCAGCGCCTGGCCCGCCGGCTTTGCTCGTGCAAGCGCGCCAGCGACCTGGCGCCCCAGGTGCTGCTC
GATGCCGGTTTCACCGATGCCGATATCGACGGCAGCTGGAAGCCGTACCACCCGGTGGGCTGCGAGCGCTGCAACGGCAG
CGGCTACAAGGGACGCTGCGGCATCTACCAGGTCATGCCCATTTCAGAGGCCATGCAGCAGATCATCCTGTCGCACGGCA
CAGCCCTGCAGATTGCCGAACAGGCCCGTAAAGAGGGCGTGCTGTCGTTGCGCGAGGCCGGGTTGCAGAAGGTAAAACAA
GGGATTACGTCGCTGGAGGAAGTGCTGGCCACCACGAACAGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-134)

Type II/IV secretion system protein (T2SSE)

(187-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

54.259

94.077

0.51
  pilB Acinetobacter baumannii D1279779

50.607

100

0.509
  pilB Acinetobacter baylyi ADP1

50.896

97.213

0.495
  pilB Legionella pneumophila strain ERS1305867

49.009

96.69

0.474
  pilB Vibrio cholerae strain A1552

45.675

100

0.46
  pilB Vibrio parahaemolyticus RIMD 2210633

45.81

93.554

0.429
  pilB Vibrio campbellii strain DS40M4

44.834

94.425

0.423
  pilF Thermus thermophilus HB27

38.368

100

0.385