Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   MMZ72_RS26830 Genome accession   NZ_CP093021
Coordinates   5801296..5801919 (-) Length   207 a.a.
NCBI ID   WP_024916549.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain H09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5796296..5806919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMZ72_RS26810 (MMZ72_26810) aroB 5796891..5797997 (-) 1107 WP_058140600.1 3-dehydroquinate synthase -
  MMZ72_RS26815 (MMZ72_26815) aroK 5798047..5798565 (-) 519 WP_003095833.1 shikimate kinase AroK -
  MMZ72_RS26820 (MMZ72_26820) pilQ 5798577..5800721 (-) 2145 WP_024916548.1 type 4a pilus secretin PilQ Machinery gene
  MMZ72_RS26825 (MMZ72_26825) comL/pilP 5800775..5801299 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  MMZ72_RS26830 (MMZ72_26830) pilO 5801296..5801919 (-) 624 WP_024916549.1 type 4a pilus biogenesis protein PilO Machinery gene
  MMZ72_RS26835 (MMZ72_26835) pilN 5801916..5802515 (-) 600 WP_024916550.1 type 4a pilus biogenesis protein PilN Machinery gene
  MMZ72_RS26840 (MMZ72_26840) pilM 5802515..5803579 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  MMZ72_RS26845 (MMZ72_26845) - 5803764..5806235 (+) 2472 WP_024916551.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22849.28 Da        Isoelectric Point: 4.7787

>NTDB_id=569712 MMZ72_RS26830 WP_024916549.1 5801296..5801919(-) (pilO) [Pseudomonas aeruginosa strain H09]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=569712 MMZ72_RS26830 WP_024916549.1 5801296..5801919(-) (pilO) [Pseudomonas aeruginosa strain H09]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTCGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCTGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCGCAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAACTGCCCAGCGACAC
CGAAGTACCCGGCCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAGATCAAGTTGCTCC
CTGAAGTCACCCAGCAGTTCTATATCGAGTTGCCGATCCAGATCAGCGTGGTCGGTGGCTACCACGACCTAGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401