Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KJA71_RS16885 Genome accession   NZ_CP075325
Coordinates   3868889..3870625 (-) Length   578 a.a.
NCBI ID   WP_011052125.1    Uniprot ID   A0AAI7ZH88
Organism   Xanthomonas citri pv. citri strain GD82     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3847187..3868047 3868889..3870625 flank 842


Gene organization within MGE regions


Location: 3847187..3870625
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJA71_RS16800 (KJA71_16800) - 3847187..3847534 (-) 348 WP_040107789.1 endonuclease domain-containing protein -
  KJA71_RS16805 (KJA71_16805) - 3847572..3848739 (+) 1168 Protein_3299 IS3 family transposase -
  KJA71_RS16810 (KJA71_16810) avrXacE2 3849499..3850569 (-) 1071 WP_011052114.1 type III secretion system effector avirulence protein AvrXacE2 -
  KJA71_RS16815 (KJA71_16815) - 3850654..3851931 (-) 1278 WP_190416505.1 lytic murein transglycosylase -
  KJA71_RS16820 (KJA71_16820) - 3852064..3855054 (-) 2991 WP_011052967.1 Tn3-like element TnXax1 family transposase -
  KJA71_RS16825 (KJA71_16825) - 3855062..3856336 (-) 1275 WP_011052968.1 tyrosine-type recombinase/integrase -
  KJA71_RS16830 (KJA71_16830) - 3856528..3857583 (+) 1056 WP_191589630.1 DNA-binding protein -
  KJA71_RS16835 (KJA71_16835) xopAI 3857716..3858606 (+) 891 WP_217377102.1 type III secretion system effector XopAI -
  KJA71_RS16840 (KJA71_16840) - 3859937..3860221 (+) 285 WP_016849322.1 hypothetical protein -
  KJA71_RS16845 (KJA71_16845) - 3860449..3861564 (+) 1116 WP_011052122.1 IS1595 family transposase -
  KJA71_RS16850 (KJA71_16850) - 3861519..3862034 (-) 516 WP_011052123.1 hypothetical protein -
  KJA71_RS16855 (KJA71_16855) sucD 3862121..3862996 (-) 876 WP_005921370.1 succinate--CoA ligase subunit alpha -
  KJA71_RS16860 (KJA71_16860) sucC 3863021..3864190 (-) 1170 WP_005915812.1 ADP-forming succinate--CoA ligase subunit beta -
  KJA71_RS16865 (KJA71_16865) - 3864422..3866035 (+) 1614 WP_003488599.1 HAMP domain-containing sensor histidine kinase -
  KJA71_RS16870 (KJA71_16870) pilR 3866360..3867754 (+) 1395 WP_015463522.1 sigma-54 dependent transcriptional regulator Regulator
  KJA71_RS16875 (KJA71_16875) - 3867977..3868144 (-) 168 WP_015463523.1 hypothetical protein -
  KJA71_RS16880 (KJA71_16880) pilB 3868276..3868395 (-) 120 WP_005915819.1 hypothetical protein Machinery gene
  KJA71_RS16885 (KJA71_16885) pilB 3868889..3870625 (-) 1737 WP_011052125.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62517.63 Da        Isoelectric Point: 5.4526

>NTDB_id=568807 KJA71_RS16885 WP_011052125.1 3868889..3870625(-) (pilB) [Xanthomonas citri pv. citri strain GD82]
MNSVVTANLVGITGIARRLVQDGAVEEAVARSAMDQASAAKVPLPQWFAEKKLVTASQLAAANAVEFGMPLLDVSAFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSALG
DDEEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAANGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=568807 KJA71_RS16885 WP_011052125.1 3868889..3870625(-) (pilB) [Xanthomonas citri pv. citri strain GD82]
ATGAATAGCGTCGTGACCGCAAATTTGGTTGGAATAACGGGCATCGCGCGTCGTCTGGTGCAAGACGGTGCGGTCGAAGA
AGCAGTTGCGCGATCTGCGATGGATCAGGCATCTGCGGCAAAGGTTCCTCTGCCGCAATGGTTTGCTGAGAAGAAGTTGG
TCACTGCTTCCCAGCTCGCCGCTGCGAATGCGGTCGAGTTCGGCATGCCACTGTTGGACGTGTCGGCGTTCGACGCCAGC
CAAAACGCGGTCAAGCTGGTAAGTGAGGAGCTACTTCAGAAGCACCAAGTGCTACCGCTGTTCAAGCGCGGCAACCGGCT
GTTCGTAGGGGTGAGCAATCCGACCCAGACCCGGGCGCTGGATGACATCAAGTTTCATACGAACTTGGTAGTCGAGCCCA
TCCTTGTGGATGAAGATCAGATCCGTCGCACCCTGGAGCAATGGCAGGCCAGCAATGCTGCGCTGGGCTCCGCGCTCGGT
GACGACGAGGAGGGCATGGGGGATCTGGACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTTGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGCTTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGGTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAACAGAAG
CTGTTCCTGGAAGCGATCCACAAGCCCTATGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTACACCGCGCTGGGAATTCTCAACGACGAGACGCGCAATATCTCCACCGCGGAGGATCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTCGCCGCGGCTTTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGTCACATGGT
GCTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGTCTGATGAACATGGGCATCGCGCCCTACAACATCA
CCTCGTCGGTGACGCTGGTGATCGCGCAGCGGCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCATTGCTGGCCGAAGGATTCACGCCTGCCCAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
TGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTAATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCGCTGATG
AAGGCTGCGAATGGGGTGACCAGCCTGGCCGAGATCAATCGTGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.514

98.27

0.555
  pilB Acinetobacter baylyi ADP1

55.81

98.27

0.548
  pilB Legionella pneumophila strain ERS1305867

52.021

98.443

0.512
  pilB Vibrio cholerae strain A1552

49.306

99.654

0.491
  pilF Neisseria gonorrhoeae MS11

49.12

98.27

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

46.964

96.886

0.455
  pilB Vibrio campbellii strain DS40M4

45.518

98.443

0.448